• 1.

    Warnick LD, Kanistanon K, McDonough PL, et al. Effect of previous antimicrobial treatment on fecal shedding of Salmonella enterica subsp. enterica serogroup B in New York dairy herds with recent clinical salmonellosis. Prev Vet Med 2003;56:285297.

    • Search Google Scholar
    • Export Citation
  • 2.

    Libby SJ, Halsey TA, Altier C, et al. In: Gyles CL, Prescott JF, Songer JG, et al, eds. Pathogenesis of bacterial infections in animals. 3rd ed. Ames, Iowa: Blackwell Publishing, 2004;143167.

    • Search Google Scholar
    • Export Citation
  • 3.

    Anderson J, Smith BP, Ulrich JT. Vaccination of calves with a modified bacterin or oil-in-water emulsion containing alkalidetoxified Salmonella typhimurium lipopolysaccharide. Am J Vet Res 1991;52:596601.

    • Search Google Scholar
    • Export Citation
  • 4.

    Da Roden L, Smith BP, Spier SJ, et al. Effect of calf age and Salmonella bacterin type on ability to produce immunoglobulins directed against Salmonella whole cells or lipopolysaccharide. Am J Vet Res 1992;53:18951899.

    • Search Google Scholar
    • Export Citation
  • 5.

    Mastroeni P, Menager N. Development of acquired immunity to Salmonella. J Med Microbiol 2003;52:453459.

  • 6.

    Selim SA, Cullor JS, Smith BP, et al. The effect of Escherichia coli J5 and modified live Salmonella dublin vaccines in artificially reared neonatal calves. Vaccine 1995;13:381390.

    • Search Google Scholar
    • Export Citation
  • 7.

    House JK, Ontiveros MM, Blackmer NM, et al. Evaluation of an autogenous Salmonella bacterin and a modified live Salmonella serotype Choleraesuis vaccine on a commercial dairy farm. Am J Vet Res 2001;62:18971902.

    • Search Google Scholar
    • Export Citation
  • 8.

    Payne SM, Finkelstein RA. The critical role of iron in host-bacterial interactions. J Clin Invest 1978;61:14281440.

  • 9.

    Huston CL, Wittum TE, Love BC, et al. Prevalence of fecal shedding of Salmonella spp in dairy herds. J Am Vet Med Assoc 2002;220:645649.

  • 10.

    Fossler CP, Wells SJ, Kaneene JB, et al. Prevalence of Salmonella spp on conventional and organic dairy farms. J Am Vet Med Assoc 2004;225:567573.

    • Search Google Scholar
    • Export Citation
  • 11.

    APHIS Web site. Animal disease exclusion practices on US dairy operations, 2002. Available at: www.aphis.usda.gov/vs/ceah/ncahs/nahms/dairy/dairy02/Dairy02_biosecurity.pdf. Accessed Jul 3, 2007.

  • 12.

    Neilands JB. Siderophores: structure and function of microbial iron transport compounds. J Biol Chem 1995;270:2672326726.

  • 13.

    Lonnerdal B, Iyer S. Lactoferrin: molecular structure and biological function. Annu Rev Nutr 1995;15:93110.

  • 14.

    Kingsley R, Rabsch W, Stephens P, et al. Iron supplying systems of Salmonella in diagnostics, epidemiology and infection. FEMS Immunol Med Microbiol 1995;11:257264.

    • Search Google Scholar
    • Export Citation
  • 15.

    Hollinger K. In: Wray C, Wray A, eds. Salmonella in domestic animals. Oxford, England: CABI Publishing, 2000;341353.

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Evaluation of vaccination with a commercial subunit vaccine on shedding of Salmonella enterica in subclinically infected dairy cows

Luke C. Heider DVM1, Richard W. Meiring DVM, DACVPM2, Armando E. Hoet DVM, PhD, DACVPM3, Wondwossen A. Gebreyes DVM, PhD, DACVPM4, Julie A. Funk DVM, PhD5, and Thomas E. Wittum PhD6
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  • 1 Department of Veterinary Preventative Medicine, College of Veterinary Medicine, The Ohio State University, Columbus, OH 43210.
  • | 2 Department of Veterinary Preventative Medicine, College of Veterinary Medicine, The Ohio State University, Columbus, OH 43210.
  • | 3 Department of Veterinary Preventative Medicine, College of Veterinary Medicine, The Ohio State University, Columbus, OH 43210.
  • | 4 Department of Veterinary Preventative Medicine, College of Veterinary Medicine, The Ohio State University, Columbus, OH 43210.
  • | 5 Department of Large Animal Clinical Sciences, College of Veterinary Medicine, Michigan State University, East Lansing, MI 48824.
  • | 6 Department of Veterinary Preventative Medicine, College of Veterinary Medicine, The Ohio State University, Columbus, OH 43210.

Abstract

Objective—To estimate the efficacy of a commercially available Salmonella enterica subunit vaccine on the subclinical shedding of S enterica in dairy cattle.

Design—Randomized, controlled trial.

Animals—175 mature cows on 2 dairy farms with a history of S enterica infection.

Procedures—25% of the mature cows from each herd were systematically randomized to receive an S enterica subunit vaccine following label guidelines. The remaining 75% of cows in each herd served as nonvaccinated controls. Fecal samples were collected from all cows at the time of initial vaccination (day 0), booster vaccination (day 14), 2 weeks following the booster vaccination (day 28), and 10 weeks following the start of the trial (day 70). All samples were processed on the day of collection and cultured for S enterica.

Results—651 fecal samples were obtained over the entire study period. Salmonella enterica was recovered from 46 (7.1%) of the samples. Shedding of S enterica was similar for vaccinated and nonvaccinated control cows on each of the collection dates.

Conclusions and Clinical Relevance—The study revealed no evidence that extralabel vaccination with a commercial subunit S enterica vaccine reduced shedding of S enterica in subclinically infected dairy cows in these herds.

Contributor Notes

Address correspondence to Dr. Heider.