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Efficacy of directed misting application of a peroxygen disinfectant for environmental decontamination of a veterinary hospital

Gage Patterson MS1, Paul S. Morley DVM, PhD, DACVIM2,3, Kenneth D. Blehm PhD4, David E. Lee DVM, MBA5, and Magda Dunowska PhD6,7
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  • 1 Animal Population Health Institute, College of Veterinary Medicine and Biomedical Sciences, Colorado State University, Fort Collins, CO 80523.
  • | 2 Animal Population Health Institute, College of Veterinary Medicine and Biomedical Sciences, Colorado State University, Fort Collins, CO 80523.
  • | 3 James L. Voss Veterinary Teaching Hospital, College of Veterinary Medicine and Biomedical Sciences, Colorado State University, Fort Collins, CO 80523.
  • | 4 Department of Environmental and Radiological Health Sciences, College of Veterinary Medicine and Biomedical Sciences, Colorado State University, Fort Collins, CO 80523.
  • | 5 James L. Voss Veterinary Teaching Hospital, College of Veterinary Medicine and Biomedical Sciences, Colorado State University, Fort Collins, CO 80523.
  • | 6 Animal Population Health Institute, College of Veterinary Medicine and Biomedical Sciences, Colorado State University, Fort Collins, CO 80523.
  • | 7 James L. Voss Veterinary Teaching Hospital, College of Veterinary Medicine and Biomedical Sciences, Colorado State University, Fort Collins, CO 80523.

Abstract

Objective—To evaluate effectiveness of 4% peroxymonosulfate disinfectant applied as a mist to surfaces in a large animal hospital as measured by recovery of Staphylococcus aureus and Salmonella enterica serovar Typhimurium.

Design—Field trial.

Sample Population—Polyester transparencies inoculated with bacteria.

Procedure—Polyester transparencies were inoculated with S aureus or S Typhimurium and placed in various locations in the hospital. After mist application of the peroxygen disinfectant, viable bacterial numbers were quantified and compared with growth from control transparencies to assess reduction in bacterial count.

Results—When applied as a mist directed at environmental surfaces contaminated with a geometric mean of 4.03 × 107 CFUs of S aureus (95% confidence interval [CI], 3.95 × 107 to 4.11 × 107) or 6.17 × 106 CFUs of S Typhimurium (95% CI, 5.55 × 106 to 6.86 × 106), 4% peroxymonosulfate reduced the geometric mean number of viable S aureus by 3.04 × 107 CFUs (95% CI, 8.6 × 105 to 1.7 × 106) and S Typhimurium by 3.97 × 106 CFUs (95% CI, 8.6 × 105 to 3.5 × 106).

Conclusions and Clinical Relevance—Environmental disinfection with directed mist application of a 4% peroxymonosulfate solution was successful in reducing counts of bacterial CFUs by > 99.9999%. Directed mist application with this peroxygen disinfectant as evaluated in this study appeared to be an effective and efficient means of environmental disinfection in a large animal veterinary hospital and would be less disruptive than more traditional approaches to intensive environmental cleaning and disinfection. (J Am Vet Med Assoc 2005;227:597–602)

Abstract

Objective—To evaluate effectiveness of 4% peroxymonosulfate disinfectant applied as a mist to surfaces in a large animal hospital as measured by recovery of Staphylococcus aureus and Salmonella enterica serovar Typhimurium.

Design—Field trial.

Sample Population—Polyester transparencies inoculated with bacteria.

Procedure—Polyester transparencies were inoculated with S aureus or S Typhimurium and placed in various locations in the hospital. After mist application of the peroxygen disinfectant, viable bacterial numbers were quantified and compared with growth from control transparencies to assess reduction in bacterial count.

Results—When applied as a mist directed at environmental surfaces contaminated with a geometric mean of 4.03 × 107 CFUs of S aureus (95% confidence interval [CI], 3.95 × 107 to 4.11 × 107) or 6.17 × 106 CFUs of S Typhimurium (95% CI, 5.55 × 106 to 6.86 × 106), 4% peroxymonosulfate reduced the geometric mean number of viable S aureus by 3.04 × 107 CFUs (95% CI, 8.6 × 105 to 1.7 × 106) and S Typhimurium by 3.97 × 106 CFUs (95% CI, 8.6 × 105 to 3.5 × 106).

Conclusions and Clinical Relevance—Environmental disinfection with directed mist application of a 4% peroxymonosulfate solution was successful in reducing counts of bacterial CFUs by > 99.9999%. Directed mist application with this peroxygen disinfectant as evaluated in this study appeared to be an effective and efficient means of environmental disinfection in a large animal veterinary hospital and would be less disruptive than more traditional approaches to intensive environmental cleaning and disinfection. (J Am Vet Med Assoc 2005;227:597–602)