Effects of positive results for Mycobacterium avium subsp paratuberculosis as determined by microbial culture of feces or antibody ELISA on results of caudal fold tuberculin test and interferon-γ assay for tuberculosis in cattle

John R. Dunn Population Medicine Center, College of Veterinary Medicine, Michigan State University, East Lansing, MI 48824.

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 DVM, MS
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John B. Kaneene Population Medicine Center, College of Veterinary Medicine, Michigan State University, East Lansing, MI 48824.

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 DVM, MPH, PhD
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Daniel L. Grooms Department of Large Animal Clinical Sciences, College of Veterinary Medicine, Michigan State University, East Lansing, MI 48824.

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Steven R. Bolin Department of Pathobiology and Diagnostic Investigation, College of Veterinary Medicine, Michigan State University, East Lansing, MI 48824.

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Carole A. Bolin Department of Pathobiology and Diagnostic Investigation, College of Veterinary Medicine, Michigan State University, East Lansing, MI 48824.

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Colleen S. Bruning-Fann Veterinary Services, Animal and Plant Health Inspection Service, USDA, 3001 Coolidge Rd, Ste 325, East Lansing, MI 48823.

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 DVM, MS

Abstract

Objective—To determine whether cattle testing positive for Mycobacterium avium subsp paratuberculosisas determined by microbial culture of feces or antibody ELISA were more likely to have false-positive responses on the caudal fold tuberculin (CFT) test or interferon-γ (IFN-γ) assay for Mycobacterium bovis than cattle testing negative for M paratuberculosis.

Animals—1,043 cattle from 10 herds in Michigan.

Procedure—Feces and blood samples for plasma were collected from cattle ≥ 24 months old on the day the CFT test was read. Fecal samples were submitted for microbial culture for M paratuberculosis. Plasma samples were tested for antibody against M paratuberculosis, and IFN-γ after stimulation with purified protein derivative tuberculin from M bovis or M avium.

Results—Of 1,043 cattle, 180 (17.3%) had positive CFT test results (suspects) and 8 (0.8%) had positive IFN-γ assay results after stimulation with purified protein derivative tuberculin from M bovis. Forty-five (4.3%) and 115 (11.0%) cattle tested positive for M paratuberculosis as determined by microbial culture of feces and antibody ELISA, respectively. Cattle with positive responses for M paratuberculosis appeared to have an increased likelihood of false-positive results on the CFT test, although this association was not significant.

Conclusions and Clinical Relevance—No significant association was detected among cattle testing positive for M paratuberculosis as determined by microbial culture of feces and antibody ELISA and positive CFT test and IFN-γ assay results for M bovis. (J Am Vet Med Assoc 2005;226:429–435)

Abstract

Objective—To determine whether cattle testing positive for Mycobacterium avium subsp paratuberculosisas determined by microbial culture of feces or antibody ELISA were more likely to have false-positive responses on the caudal fold tuberculin (CFT) test or interferon-γ (IFN-γ) assay for Mycobacterium bovis than cattle testing negative for M paratuberculosis.

Animals—1,043 cattle from 10 herds in Michigan.

Procedure—Feces and blood samples for plasma were collected from cattle ≥ 24 months old on the day the CFT test was read. Fecal samples were submitted for microbial culture for M paratuberculosis. Plasma samples were tested for antibody against M paratuberculosis, and IFN-γ after stimulation with purified protein derivative tuberculin from M bovis or M avium.

Results—Of 1,043 cattle, 180 (17.3%) had positive CFT test results (suspects) and 8 (0.8%) had positive IFN-γ assay results after stimulation with purified protein derivative tuberculin from M bovis. Forty-five (4.3%) and 115 (11.0%) cattle tested positive for M paratuberculosis as determined by microbial culture of feces and antibody ELISA, respectively. Cattle with positive responses for M paratuberculosis appeared to have an increased likelihood of false-positive results on the CFT test, although this association was not significant.

Conclusions and Clinical Relevance—No significant association was detected among cattle testing positive for M paratuberculosis as determined by microbial culture of feces and antibody ELISA and positive CFT test and IFN-γ assay results for M bovis. (J Am Vet Med Assoc 2005;226:429–435)

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