Evaluation of a point-of-care blood analyzer and determination of reference ranges for blood parameters in rockfish

Lisa A. Harrenstien Oregon Zoo, 4001 SW Canyon Rd, Portland, OR 97221.

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Susan J. Tornquist Department of Biomedical Sciences, College of Veterinary Medicine, Oregon State University, Corvallis, OR 97331.

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Timothy J. Miller-Morgan Department of Biomedical Sciences, College of Veterinary Medicine, Oregon State University, Corvallis, OR 97331.
Oregon Coast Aquarium, 2820 SE Ferry Slip Rd, Newport, OR 97365.
Hatfield Marine Science Center, Oregon Extension Sea Grant, Oregon State University, Newport, OR 97365.

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Brian G. Fodness Oregon Coast Aquarium, 2820 SE Ferry Slip Rd, Newport, OR 97365.

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Kevin E. Clifford Oregon Coast Aquarium, 2820 SE Ferry Slip Rd, Newport, OR 97365.

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Abstract

Objective—To compare values of blood parameters in rockfish obtained by use of a point-of-care portable blood analyzer with values determined by a veterinary diagnostic laboratory, calculate reference ranges for various blood parameters in black rockfish, and compare values of blood parameters in clinically normal fish with those of fish with clinical abnormalities.

Design—Prospective study.

Animals—41 captive adult black rockfish (Sebastes melanops) and 4 captive adult blue rockfish (Sebastes mystinus).

Procedure—Rockfish were anesthetized with tricaine methanesulfonate for collection of blood samples. Heparinized blood samples were immediately analyzed with a point-of-care analyzer. Blood sodium, potassium, chloride, urea nitrogen, and glucose concentrations; Hct; pH; partial pressure of carbon dioxide; total carbon dioxide concentration; bicarbonate concentration; base excess; and hemoglobin concentration were determined. A microhematocrit technique was used to determine PCV, and a refractometer was used to estimate total plasma protein concentration. Paired heparinized blood samples were transported to a veterinary diagnostic laboratory for analyses.

Results—Data obtained with the point-of-care analyzer were reproducible; however, values for most blood parameters were significantly different from those obtained by the veterinary diagnostic laboratory. Fish with poor body condition had several blood parameter values that were lower than corresponding values in clinically normal fish.

Conclusions and Clinical Relevance—Point-of-care blood analyses may prove useful in rockfish. Point-of-care data for a large number of clinically normal fish must be obtained for reference ranges to be calculated, and further assessments of clinically abnormal fish are necessary to determine the relevance of the data. (J Am Vet Med Assoc 2005;226:255–265)

Abstract

Objective—To compare values of blood parameters in rockfish obtained by use of a point-of-care portable blood analyzer with values determined by a veterinary diagnostic laboratory, calculate reference ranges for various blood parameters in black rockfish, and compare values of blood parameters in clinically normal fish with those of fish with clinical abnormalities.

Design—Prospective study.

Animals—41 captive adult black rockfish (Sebastes melanops) and 4 captive adult blue rockfish (Sebastes mystinus).

Procedure—Rockfish were anesthetized with tricaine methanesulfonate for collection of blood samples. Heparinized blood samples were immediately analyzed with a point-of-care analyzer. Blood sodium, potassium, chloride, urea nitrogen, and glucose concentrations; Hct; pH; partial pressure of carbon dioxide; total carbon dioxide concentration; bicarbonate concentration; base excess; and hemoglobin concentration were determined. A microhematocrit technique was used to determine PCV, and a refractometer was used to estimate total plasma protein concentration. Paired heparinized blood samples were transported to a veterinary diagnostic laboratory for analyses.

Results—Data obtained with the point-of-care analyzer were reproducible; however, values for most blood parameters were significantly different from those obtained by the veterinary diagnostic laboratory. Fish with poor body condition had several blood parameter values that were lower than corresponding values in clinically normal fish.

Conclusions and Clinical Relevance—Point-of-care blood analyses may prove useful in rockfish. Point-of-care data for a large number of clinically normal fish must be obtained for reference ranges to be calculated, and further assessments of clinically abnormal fish are necessary to determine the relevance of the data. (J Am Vet Med Assoc 2005;226:255–265)

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