Diversity of Salmonella serotypes in cull (market) dairy cows at slaughter

John C. Galland Food Animal Health and Management Center, College of Veterinary Medicine, Kansas State University, Manhattan, KS 66506.

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H. Fred Troutt Food Animal Production Medicine Consortium, Department of Veterinary Clinical Medicine, College of Veterinary Medicine, University of Illinois, Urbana, IL 61802.

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Robert L. Brewer USDA-FSIS, OPHS, Rm 392, Aerospace Center, Washington, DC 20250.

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Bennie I. Osburn Department of Pathology, Microbiology, and Immunology, School of Veterinary Medicine, University of California, Davis, CA 95616.

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R. Kenneth Braun Department of Large Animal Clinical Sciences, College of Veterinary Medicine, University of Florida, Gainesville, FL 32610.

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Phil Sears Department of Large Animal Clinical Sciences, College of Veterinary Medicine, Michigan State University, East Lansing, MI 48824.

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John A. Schmitz Department of Veterinary and Biomedical Sciences, University of Nebraska, Lincoln, NE 68583.

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Asa B. Childers Department of Veterinary Anatomy and Public Health, College of Veterinary Medicine, Texas A&M University, College Station, TX 77843.

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Ed Richey Department of Large Animal Clinical Sciences, College of Veterinary Medicine, University of Florida, Gainesville, FL 32610.

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Kris Murthy USDA-FSIS, OPHS, Rm 392, Aerospace Center, Washington, DC 20250.

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Edward Mather Department of Large Animal Clinical Sciences, College of Veterinary Medicine, Michigan State University, East Lansing, MI 48824.

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Michael Gibson Department of Veterinary Anatomy and Public Health, College of Veterinary Medicine, Texas A&M University, College Station, TX 77843.

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Abstract

Objective—To determine the diversity of Salmonella serotypes isolated from a large population of cull (market) dairy cows at slaughter.

Design—Cross-sectional study.

Sample PopulationSalmonella organisms isolated from the cecal-colon contents of 5,087 market dairy cows.

Procedure—During winter and summer 1996, cecalcolon contents of cull dairy cows at slaughter were obtained from 5 US slaughter establishments. Specimens were subjected to microbiologic culturing for Salmonella spp at 1 laboratory. Identified isolates were compared with Salmonella isolation lists published by the Centers for Disease Control and Prevention (CDC) and the National Veterinary Services Laboratory (NVSL) for approximately the same period. The Simpson diversity index was used to calculate the likelihood that Salmonella isolates selected randomly by establishment were different.

Results—Of 58 Salmonella serotypes identified, Salmonella ser. Montevideo was the most prevalent. Two of the top 10 CDC serotypes identified from humans in 1996, Salmonella ser. Typhimurium and S Montevideo, appeared on our top 10 list; 8 of the top 10 were found on NVSL listings. Thirty-one of 59 S Typhimurium isolates were identified as DT104 and found at a west slaughter establishment, 30 during the winter and 1 during the summer. The greatest diversity of serotypes was at a southeast establishment during the summer; the least diversity was at a central establishment in the winter.

Conclusions and Clinical Relevance—58 Salmonella serotypes were isolated from market dairy cows at slaughter and could pose a threat for food-borne illness. Salmonella Montevideo was the most frequently isolated serotype and may contribute substantially to salmonellosis in dairy cattle. (J Am Vet Med Assoc 2001;219:1216–1220)

Abstract

Objective—To determine the diversity of Salmonella serotypes isolated from a large population of cull (market) dairy cows at slaughter.

Design—Cross-sectional study.

Sample PopulationSalmonella organisms isolated from the cecal-colon contents of 5,087 market dairy cows.

Procedure—During winter and summer 1996, cecalcolon contents of cull dairy cows at slaughter were obtained from 5 US slaughter establishments. Specimens were subjected to microbiologic culturing for Salmonella spp at 1 laboratory. Identified isolates were compared with Salmonella isolation lists published by the Centers for Disease Control and Prevention (CDC) and the National Veterinary Services Laboratory (NVSL) for approximately the same period. The Simpson diversity index was used to calculate the likelihood that Salmonella isolates selected randomly by establishment were different.

Results—Of 58 Salmonella serotypes identified, Salmonella ser. Montevideo was the most prevalent. Two of the top 10 CDC serotypes identified from humans in 1996, Salmonella ser. Typhimurium and S Montevideo, appeared on our top 10 list; 8 of the top 10 were found on NVSL listings. Thirty-one of 59 S Typhimurium isolates were identified as DT104 and found at a west slaughter establishment, 30 during the winter and 1 during the summer. The greatest diversity of serotypes was at a southeast establishment during the summer; the least diversity was at a central establishment in the winter.

Conclusions and Clinical Relevance—58 Salmonella serotypes were isolated from market dairy cows at slaughter and could pose a threat for food-borne illness. Salmonella Montevideo was the most frequently isolated serotype and may contribute substantially to salmonellosis in dairy cattle. (J Am Vet Med Assoc 2001;219:1216–1220)

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