Serologic prevalence of Sarcocystis neurona, Toxoplasma gondii, and Neospora caninum in horses in Brazil

J. P. Dubey From the Parasite Biology and Epidemiology Laboratory, United States Department of Agriculture, Agricultural Research Service, Livestock and Poultry Sciences Institute, Beltsville Agricultural Research Center, Beltsville, MD 20705-2350 (Dubey); Paddock Laboratorio de Anialises, Clinicas Veterinarias, Rua Deputado Bady Bassit, 300-CEP 05517-050, Sao Paulo, Brazil (Kerber); and the Department of Veterinary Science, Gluck Equine Research Center, University of Kentucky, Lexington, KY 40546 (Granstrom).

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Claudia E. Kerber From the Parasite Biology and Epidemiology Laboratory, United States Department of Agriculture, Agricultural Research Service, Livestock and Poultry Sciences Institute, Beltsville Agricultural Research Center, Beltsville, MD 20705-2350 (Dubey); Paddock Laboratorio de Anialises, Clinicas Veterinarias, Rua Deputado Bady Bassit, 300-CEP 05517-050, Sao Paulo, Brazil (Kerber); and the Department of Veterinary Science, Gluck Equine Research Center, University of Kentucky, Lexington, KY 40546 (Granstrom).

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David E. Granstrom From the Parasite Biology and Epidemiology Laboratory, United States Department of Agriculture, Agricultural Research Service, Livestock and Poultry Sciences Institute, Beltsville Agricultural Research Center, Beltsville, MD 20705-2350 (Dubey); Paddock Laboratorio de Anialises, Clinicas Veterinarias, Rua Deputado Bady Bassit, 300-CEP 05517-050, Sao Paulo, Brazil (Kerber); and the Department of Veterinary Science, Gluck Equine Research Center, University of Kentucky, Lexington, KY 40546 (Granstrom).

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Objective

To determine serologic prevalence of Sarcocystis neurona, Toxoplasma gondii, and Neospora caninum in horses in Brazil.

Design

Prevalence survey.

Animals

101 Thoroughbreds in Brazil.

Procedure

Blood samples were obtained from horses and tested for serum antibodies against S neurona by use of an immunoblot procedure with culture-derived S neurona merozoites as antigen, and for serum antibodies against T gondii and N caninum by use of a modified agglutination test with formalinpreserved tachyzoites and mercaptoethanol.

Results

Antibodies against S neurona and T gondii were detected in 36 and 16 of 101 horses, respectively. Cross-reactivity between antibodies against T gondii and S neurona was not detected. Antibodies against N caninum were not detected in any samples.

Conclusions and Clinical Relevance

The high prevalence of antibodies against S neurona detected in clinically normal horses emphasizes the importance of examining CSF for antibodies when establishing a diagnosis of equine protozoal myeloencephalitis. (J Am Vet Med Assoc 1999;215:970–972)

Objective

To determine serologic prevalence of Sarcocystis neurona, Toxoplasma gondii, and Neospora caninum in horses in Brazil.

Design

Prevalence survey.

Animals

101 Thoroughbreds in Brazil.

Procedure

Blood samples were obtained from horses and tested for serum antibodies against S neurona by use of an immunoblot procedure with culture-derived S neurona merozoites as antigen, and for serum antibodies against T gondii and N caninum by use of a modified agglutination test with formalinpreserved tachyzoites and mercaptoethanol.

Results

Antibodies against S neurona and T gondii were detected in 36 and 16 of 101 horses, respectively. Cross-reactivity between antibodies against T gondii and S neurona was not detected. Antibodies against N caninum were not detected in any samples.

Conclusions and Clinical Relevance

The high prevalence of antibodies against S neurona detected in clinically normal horses emphasizes the importance of examining CSF for antibodies when establishing a diagnosis of equine protozoal myeloencephalitis. (J Am Vet Med Assoc 1999;215:970–972)

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