Use of composite milk samples for diagnosis of Staphylococcus aureus mastitis in dairy cattle

T. J. G. M. Lam From the Department of Herd Health and Reproduction, Faculty of Veterinary Medicine, Utrecht University, PO Box 80.151, 3508 TD Utrecht (Lam, Morselt, Schukken), and the Animal Health Service East Netherlands, PO Box 9, 7400 AA Deventer, The Netherlands, (van Wuijckhuise, Franken, Hartman).

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L. A. van Wuijckhuise From the Department of Herd Health and Reproduction, Faculty of Veterinary Medicine, Utrecht University, PO Box 80.151, 3508 TD Utrecht (Lam, Morselt, Schukken), and the Animal Health Service East Netherlands, PO Box 9, 7400 AA Deventer, The Netherlands, (van Wuijckhuise, Franken, Hartman).

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P. Franken From the Department of Herd Health and Reproduction, Faculty of Veterinary Medicine, Utrecht University, PO Box 80.151, 3508 TD Utrecht (Lam, Morselt, Schukken), and the Animal Health Service East Netherlands, PO Box 9, 7400 AA Deventer, The Netherlands, (van Wuijckhuise, Franken, Hartman).

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M. L. Morselt From the Department of Herd Health and Reproduction, Faculty of Veterinary Medicine, Utrecht University, PO Box 80.151, 3508 TD Utrecht (Lam, Morselt, Schukken), and the Animal Health Service East Netherlands, PO Box 9, 7400 AA Deventer, The Netherlands, (van Wuijckhuise, Franken, Hartman).

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E. G. Hartman From the Department of Herd Health and Reproduction, Faculty of Veterinary Medicine, Utrecht University, PO Box 80.151, 3508 TD Utrecht (Lam, Morselt, Schukken), and the Animal Health Service East Netherlands, PO Box 9, 7400 AA Deventer, The Netherlands, (van Wuijckhuise, Franken, Hartman).

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Y. H. Schukken From the Department of Herd Health and Reproduction, Faculty of Veterinary Medicine, Utrecht University, PO Box 80.151, 3508 TD Utrecht (Lam, Morselt, Schukken), and the Animal Health Service East Netherlands, PO Box 9, 7400 AA Deventer, The Netherlands, (van Wuijckhuise, Franken, Hartman).

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Objective

To measure relative sensitivity and relative specificity for use of composite milk samples, compared with that of individual gland milk samples, for diagnosis of Staphylococcus aureus mastitis.

Animals

505 cows suspected of having subclinical mastitis. Of these cows, 172 were considered infected with Sta aureus, based on the results from individual gland samples.

Procedure

Composite and individual gland milk samples were collected from cows suspected of having subclinical mastitis, and results of bacteriologic culturing of samples from the same cow were compared. Results were interpreted at the cow level. Relative sensitivity and relative specificity for composite samples were computed from 2 × 2 tables, using results from individual gland samples as references.

Results

Relative sensitivity for use of composite milk samples in diagnosing Sta aureus mastitis was 0.63. The relative specificity was 0.98. Factors influencing the relative sensitivity for composite samples were the number of infected glands per cow, the amount of Sta aureus shedding from infected glands, and the proportion of the composite milk obtained from each gland.

Clinical Implications

Collecting composite instead of individual gland milk samples increases the number of false-negative results in diagnosing Sta aureus mastitis. By collecting consecutive samples from the same cow or by increasing the inoculum volume at culturing, this problem can be diminished. (J Am Med Assoc 1996; 208:1705-1708)

Objective

To measure relative sensitivity and relative specificity for use of composite milk samples, compared with that of individual gland milk samples, for diagnosis of Staphylococcus aureus mastitis.

Animals

505 cows suspected of having subclinical mastitis. Of these cows, 172 were considered infected with Sta aureus, based on the results from individual gland samples.

Procedure

Composite and individual gland milk samples were collected from cows suspected of having subclinical mastitis, and results of bacteriologic culturing of samples from the same cow were compared. Results were interpreted at the cow level. Relative sensitivity and relative specificity for composite samples were computed from 2 × 2 tables, using results from individual gland samples as references.

Results

Relative sensitivity for use of composite milk samples in diagnosing Sta aureus mastitis was 0.63. The relative specificity was 0.98. Factors influencing the relative sensitivity for composite samples were the number of infected glands per cow, the amount of Sta aureus shedding from infected glands, and the proportion of the composite milk obtained from each gland.

Clinical Implications

Collecting composite instead of individual gland milk samples increases the number of false-negative results in diagnosing Sta aureus mastitis. By collecting consecutive samples from the same cow or by increasing the inoculum volume at culturing, this problem can be diminished. (J Am Med Assoc 1996; 208:1705-1708)

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