Diagnosis of induced toxoplasmosis in neonatal cats

J. P. Dubey From the Parasite Biology and Epidemiology Laboratory, Livestock and Poultry Sciences Institute, Agricultural Research Service, USDA, Beltsville, MD 20705-2350 (Dubey); Department of Clinical Sciences, College of Veterinary Medicine and Biomedical Sciences. Colorado Siaic University. Foil Collins, CO 80523 (bippin); and Laboratoire de la toxoplasmose. Institut de Puériculture de Paris 26, Boulevard Brune, 75014 Paris, France (Thulliez).

Search for other papers by J. P. Dubey in
Current site
Google Scholar
PubMed
Close
 MVSc, PhD
,
M. R. Lappin From the Parasite Biology and Epidemiology Laboratory, Livestock and Poultry Sciences Institute, Agricultural Research Service, USDA, Beltsville, MD 20705-2350 (Dubey); Department of Clinical Sciences, College of Veterinary Medicine and Biomedical Sciences. Colorado Siaic University. Foil Collins, CO 80523 (bippin); and Laboratoire de la toxoplasmose. Institut de Puériculture de Paris 26, Boulevard Brune, 75014 Paris, France (Thulliez).

Search for other papers by M. R. Lappin in
Current site
Google Scholar
PubMed
Close
 DVM, PhD
, and
P. Thulliez From the Parasite Biology and Epidemiology Laboratory, Livestock and Poultry Sciences Institute, Agricultural Research Service, USDA, Beltsville, MD 20705-2350 (Dubey); Department of Clinical Sciences, College of Veterinary Medicine and Biomedical Sciences. Colorado Siaic University. Foil Collins, CO 80523 (bippin); and Laboratoire de la toxoplasmose. Institut de Puériculture de Paris 26, Boulevard Brune, 75014 Paris, France (Thulliez).

Search for other papers by P. Thulliez in
Current site
Google Scholar
PubMed
Close
 MD

Click on author name to view affiliation information

Summary

Sixteen pregnant queens were inoculated orally with tissue cysts of Toxoplasma gondii, and fetal membranes and offspring were examined for Τ gondii infection by bioassay in mice. Queens appeared clinically normal, although all shed Τ gondii oocysts. Toxoplasma gondii was isolated from tissues of 7 of 33 fetuses or kittens from 5 litters (at 13, 23, 26, 27, and 29 postinaculation days) from 8 queens euthanatized between 10 and 31 postinoculation days. Infection with Τ gondii was found In kittens from all 8 litters from the 8 queens that were allowed to undergo parturition and nurse their kittens. A total of 43 kittens were born to these 8 quean.

Toxoplasma gondii was isolated from tissues of 26 of 40 kittens bioassayed; in 3 kittens, tissues were not available for bioassay. Toxoplasmosis was severe in full-term kittens born to 5 queens; all 25 kittens from these litters died or were ill by 24 days of age. Anorexia, lethargy, hypothermia, and sudden death were the most common manifestations. Cytologic examination of peritoneal fluid aspirate samples and determination of hepatic-associated enzyme concentrations in affected kittens, as well as measurement of anti-T gondii antibodies in serum of kittens and queens, were helpful in the diagnosis of neonatal toxoplasmosis. Transplacental transfer of anti-T gondii antibodies was not observed in cats. Toxoplasma gondii oocysts were found in fecal samples of 3 kittens from different litters at 16, 24, and 63 days of age.

Summary

Sixteen pregnant queens were inoculated orally with tissue cysts of Toxoplasma gondii, and fetal membranes and offspring were examined for Τ gondii infection by bioassay in mice. Queens appeared clinically normal, although all shed Τ gondii oocysts. Toxoplasma gondii was isolated from tissues of 7 of 33 fetuses or kittens from 5 litters (at 13, 23, 26, 27, and 29 postinaculation days) from 8 queens euthanatized between 10 and 31 postinoculation days. Infection with Τ gondii was found In kittens from all 8 litters from the 8 queens that were allowed to undergo parturition and nurse their kittens. A total of 43 kittens were born to these 8 quean.

Toxoplasma gondii was isolated from tissues of 26 of 40 kittens bioassayed; in 3 kittens, tissues were not available for bioassay. Toxoplasmosis was severe in full-term kittens born to 5 queens; all 25 kittens from these litters died or were ill by 24 days of age. Anorexia, lethargy, hypothermia, and sudden death were the most common manifestations. Cytologic examination of peritoneal fluid aspirate samples and determination of hepatic-associated enzyme concentrations in affected kittens, as well as measurement of anti-T gondii antibodies in serum of kittens and queens, were helpful in the diagnosis of neonatal toxoplasmosis. Transplacental transfer of anti-T gondii antibodies was not observed in cats. Toxoplasma gondii oocysts were found in fecal samples of 3 kittens from different litters at 16, 24, and 63 days of age.

All Time Past Year Past 30 Days
Abstract Views 0 0 0
Full Text Views 457 457 110
PDF Downloads 69 69 6
Advertisement