Production, characterization, and applications of a murine monoclonal antibody to dog erythrocyte antigen 1.1

Gordon A. Andrews From the Department of Veterinary Pathology and Microbiology, College of Veterinary Medicine, Kansas State University, Manhattan, KS 66506-5605.

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Patricia S. Chavey From the Department of Veterinary Pathology and Microbiology, College of Veterinary Medicine, Kansas State University, Manhattan, KS 66506-5605.

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Joseph E. Smith From the Department of Veterinary Pathology and Microbiology, College of Veterinary Medicine, Kansas State University, Manhattan, KS 66506-5605.

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Summary

A murine IgM monoclonal antibody, which recognizes dog erythrocyte antigen (dea) 1.1, has been produced. The antibody correctly identified canine rbc possessing dea 1.1 in a panel of rbc typed by an independent laboratory. Reactivity of the monoclonal antibody was compared with canine anti-dea 1.1 antiserum with 163 rbc samples from 145 dogs. Results of agglutination tests with the 2 reagents were in agreement for all samples. A card agglutination test that uses the monoclonal antibody with blood is described. A monoclonal antibody-based test should facilitate blood typing for dea 1.1 in clinical practice.

Summary

A murine IgM monoclonal antibody, which recognizes dog erythrocyte antigen (dea) 1.1, has been produced. The antibody correctly identified canine rbc possessing dea 1.1 in a panel of rbc typed by an independent laboratory. Reactivity of the monoclonal antibody was compared with canine anti-dea 1.1 antiserum with 163 rbc samples from 145 dogs. Results of agglutination tests with the 2 reagents were in agreement for all samples. A card agglutination test that uses the monoclonal antibody with blood is described. A monoclonal antibody-based test should facilitate blood typing for dea 1.1 in clinical practice.

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