Use of an enzyme-linked immunosorbent assay for detection of infection with pseudorabies virus on a herd basis

Sergio J. Duffy From the Department of Clinical and Population Sciences, College of Veterinary Medicine, University of Minnesota, St Paul, MN 55108.

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 DVM, PhD
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Robert B. Morrison From the Department of Clinical and Population Sciences, College of Veterinary Medicine, University of Minnesota, St Paul, MN 55108.

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Sagar M. Goyal From the Department of Clinical and Population Sciences, College of Veterinary Medicine, University of Minnesota, St Paul, MN 55108.

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 BVSc, PhD

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Summary

The use of an elisa that can differentiate between swine infected with pseudorabies virus (prv) and swine vaccinated with a specific prv vaccine was evaluated on an individual and herd basis, and a system for interpreting elisa results on a herd basis was developed. In 17 herds, recently introduced replacement gilts, seronegative for prv, were vaccinated with a thymidine kinase- and glycoprotein X (gpX)-deleted vaccine. After vaccination, blood samples were collected from these gilts approximately every 1 to 2 months for up to 19 months. Serum samples were analyzed for antibodies to gpX antigen, using a commercially available elisa kit according to the manufacturer's protocol. Herd status was determined as positive, suspect, or negative, according to the serum sample:negative control (s:n) values of the samples collected from the herd. From the 17 herds, 130 evaluations were performed. On 49 (38%) of the 130 herd evaluations, 1 or more gilts had suspect test results. Additional testing was required in 19 (39%) of these 49 herd evaluations to determine the prv infection status of the herd. Status of herds having gilts with suspect results and no positive results was usually negative after retesting. Herds having gilts with positive results were unlikely to have negative status after retesting.

Summary

The use of an elisa that can differentiate between swine infected with pseudorabies virus (prv) and swine vaccinated with a specific prv vaccine was evaluated on an individual and herd basis, and a system for interpreting elisa results on a herd basis was developed. In 17 herds, recently introduced replacement gilts, seronegative for prv, were vaccinated with a thymidine kinase- and glycoprotein X (gpX)-deleted vaccine. After vaccination, blood samples were collected from these gilts approximately every 1 to 2 months for up to 19 months. Serum samples were analyzed for antibodies to gpX antigen, using a commercially available elisa kit according to the manufacturer's protocol. Herd status was determined as positive, suspect, or negative, according to the serum sample:negative control (s:n) values of the samples collected from the herd. From the 17 herds, 130 evaluations were performed. On 49 (38%) of the 130 herd evaluations, 1 or more gilts had suspect test results. Additional testing was required in 19 (39%) of these 49 herd evaluations to determine the prv infection status of the herd. Status of herds having gilts with suspect results and no positive results was usually negative after retesting. Herds having gilts with positive results were unlikely to have negative status after retesting.

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