Estimation of the sensitivity and specificity of the agar gel immunodiffusion test for bovine leukemia virus: 1,296 cases (1982-1989)

Donald R. Monke From the Veterinary Department, Select Sires Inc, Plain City, OH 43064-0143.

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Ronald F. Rohde From the Veterinary Department, Select Sires Inc, Plain City, OH 43064-0143.

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William D. Hueston From the Veterinary Department, Select Sires Inc, Plain City, OH 43064-0143.

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Robert J. Milburn From the Veterinary Department, Select Sires Inc, Plain City, OH 43064-0143.

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Summary

A retrospective study of the results of 12,549 agar gel immunodiffusion tests for bovine leukemia virus, conducted on 1,296 dairy bulls over an 8-year period, was performed to estimate the sensitivity and specificity of the test. The number of tests performed on each bull ranged from 5 to 35, with a mean of 9.7 tests per bull.

Bulls were categorized by their agar gel immunodiffusion test responses; 1,069 (82.5%) were non-infected and 227 (17.5%) were infected. Eighteen false-positive results were reported from the noninfected bulls. Test specificity was estimated to be 99.8%. Thirty-one false-negative results were reported from the infected bulls. Test sensitivity was estimated to be 98.5%.

Fifty-six bulls had 1 or more positive responses when < 6 months old. In 26 (46%), these results were thought to be attributable to colostral immunity.

Summary

A retrospective study of the results of 12,549 agar gel immunodiffusion tests for bovine leukemia virus, conducted on 1,296 dairy bulls over an 8-year period, was performed to estimate the sensitivity and specificity of the test. The number of tests performed on each bull ranged from 5 to 35, with a mean of 9.7 tests per bull.

Bulls were categorized by their agar gel immunodiffusion test responses; 1,069 (82.5%) were non-infected and 227 (17.5%) were infected. Eighteen false-positive results were reported from the noninfected bulls. Test specificity was estimated to be 99.8%. Thirty-one false-negative results were reported from the infected bulls. Test sensitivity was estimated to be 98.5%.

Fifty-six bulls had 1 or more positive responses when < 6 months old. In 26 (46%), these results were thought to be attributable to colostral immunity.

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