Use of western blot and radioimmunoprecipitation for diagnosis of feline leukemia and feline immunodeficiency virus infections

Herman F. Egberink From the Department of Infectious Diseases and Immunology, Veterinary Faculty, Institute of Virology, Yalelaan 1, 3584 CL Utrecht, The Netherlands (Egberink, Horzinek) and the Veterinary Faculty, University of Zurich, Winterthurer Str 269, 8057 Zürich, Switzerland (Lutz).

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Hans Lutz From the Department of Infectious Diseases and Immunology, Veterinary Faculty, Institute of Virology, Yalelaan 1, 3584 CL Utrecht, The Netherlands (Egberink, Horzinek) and the Veterinary Faculty, University of Zurich, Winterthurer Str 269, 8057 Zürich, Switzerland (Lutz).

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Marian C. Horzinek From the Department of Infectious Diseases and Immunology, Veterinary Faculty, Institute of Virology, Yalelaan 1, 3584 CL Utrecht, The Netherlands (Egberink, Horzinek) and the Veterinary Faculty, University of Zurich, Winterthurer Str 269, 8057 Zürich, Switzerland (Lutz).

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Summary

The uses and limitations of the western blot (wb) and radioimmunoprecipitation assay (ripa) techniques for study of feline immunodeficiency virus (fiv) and FeLV were evaluated. Western blot analysis was used to detect antigenic relatedness between the 2 lentiviruses. Using a rabbit serum directed against p26 of the equine infectious anemia virus (eiav) and anti-eiav horse serum obtained from an infected horse, cross-reactivity with p24 of fiv was revealed. Cat sera obtained late after experimentally induced fiv infection recognized p26 of eiav, which indicates reciprocal cross-reactivity.

Summary

The uses and limitations of the western blot (wb) and radioimmunoprecipitation assay (ripa) techniques for study of feline immunodeficiency virus (fiv) and FeLV were evaluated. Western blot analysis was used to detect antigenic relatedness between the 2 lentiviruses. Using a rabbit serum directed against p26 of the equine infectious anemia virus (eiav) and anti-eiav horse serum obtained from an infected horse, cross-reactivity with p24 of fiv was revealed. Cat sera obtained late after experimentally induced fiv infection recognized p26 of eiav, which indicates reciprocal cross-reactivity.

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