Use of an enzyme-linked immunosorbent assay to estimate prevalence of paratuberculosis in cattle of Florida

R. Kenneth Braun From the Departments of Large Animal Clinical Sciences (Braun) and Comparative and Experimental Pathology (Buergelt), College of Veterinary Medicine, and the Departments of Statistics (Littell, Linda) and Food and Resource Economics (Simpson), Institute of Food and Agricultural Sciences, University of Florida, Gainesville, FL 32610.

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 DVM, MS
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Claus D. Buergelt From the Departments of Large Animal Clinical Sciences (Braun) and Comparative and Experimental Pathology (Buergelt), College of Veterinary Medicine, and the Departments of Statistics (Littell, Linda) and Food and Resource Economics (Simpson), Institute of Food and Agricultural Sciences, University of Florida, Gainesville, FL 32610.

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Ramon C. Littell From the Departments of Large Animal Clinical Sciences (Braun) and Comparative and Experimental Pathology (Buergelt), College of Veterinary Medicine, and the Departments of Statistics (Littell, Linda) and Food and Resource Economics (Simpson), Institute of Food and Agricultural Sciences, University of Florida, Gainesville, FL 32610.

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Stephen B. Linda From the Departments of Large Animal Clinical Sciences (Braun) and Comparative and Experimental Pathology (Buergelt), College of Veterinary Medicine, and the Departments of Statistics (Littell, Linda) and Food and Resource Economics (Simpson), Institute of Food and Agricultural Sciences, University of Florida, Gainesville, FL 32610.

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James R. Simpson From the Departments of Large Animal Clinical Sciences (Braun) and Comparative and Experimental Pathology (Buergelt), College of Veterinary Medicine, and the Departments of Statistics (Littell, Linda) and Food and Resource Economics (Simpson), Institute of Food and Agricultural Sciences, University of Florida, Gainesville, FL 32610.

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Summary

A survey was conducted from 1986 through 1987, for which an ELISA was used to obtain information on the prevalence of Mycobacterium paratuberculosis infection in cattle of Florida. Results revealed prevalence of 8.6% in beef cattle and 17.1% in dairy cattle. In beef and dairy cattle, prevalence increased with increasing herd size. It was concluded that ELISA-detectable circulating antibodies to M paratuberculosis are widespread in cattle of Florida.

Summary

A survey was conducted from 1986 through 1987, for which an ELISA was used to obtain information on the prevalence of Mycobacterium paratuberculosis infection in cattle of Florida. Results revealed prevalence of 8.6% in beef cattle and 17.1% in dairy cattle. In beef and dairy cattle, prevalence increased with increasing herd size. It was concluded that ELISA-detectable circulating antibodies to M paratuberculosis are widespread in cattle of Florida.

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