Veterinary-specific PBGMs are commonly used to monitor systemic blood glucose concentrations of dogs and cats in veterinary hospitals and other settings.1–4 These devices are particularly useful when frequent measurements are required because they provide a rapid assessment of systemic blood glucose concentration, are readily available and cost-effective, and require as little as 0.3 μL of blood.5–7 Studies5,7 in which results for veterinary-specific PBGMs were compared with results for hexokinase reference methods revealed that a number of these machines provide clinically acceptable results when peripheral venous or capillary blood samples are used.
The PBGM that has been used at the authors’ institution is a second-generation veterinary-specific glucometer that involves electrochemical technology and a glucose dehydrogenase reagent to measure blood glucose concentrations in samples obtained from dogs and cats. Studies5,8 conducted to compare results for this device with results for other PBGMs revealed that the PBGM used at our institution had among the lowest bias and greatest accuracy for measurement of glucose concentrations in venous blood and control solutions. The manufacturer recommends the use of blood collected from a peripheral vein or capillary blood collected from a marginal ear vein, non-weight-bearing portion of a paw pad, oral mucosa, or hyperkeratotic skin overlying the elbow joint.9,10 Measurements of blood glucose concentrations in samples obtained from a carpal pad, pinna, and peripheral vein of diabetic dogs were all considered clinically acceptable.11 However, similar comparisons are lacking for healthy dogs and cats as well as dogs and cats with nondiabetic illnesses.
In a number of nondiabetic patients at the authors’ institution, there have been clinically important disparities between the venous and capillary blood glucose concentrations measured with a PBGM. These differences have led to concerns about the effect of sample collection site on the results. One possible cause of these discrepancies may have been the prandial state of patients, which could have affected glucose concentrations in capillary and venous blood. Studies of mice12 and humans13 have revealed asynchronous shifts in blood glucose concentrations in venous and capillary blood samples obtained before and after eating. However, similar studies of dogs are lacking.
The purpose of the study reported here was to compare glucose concentrations measured with a veterinary-specific PBGM in capillary and peripheral venous blood samples obtained from healthy dogs and to determine whether those concentrations were affected by the prandial state of the dogs. We hypothesized that the site of sample collection and prandial state would not cause a significant difference in blood glucose concentrations.
Supported by the Companion Animal Fund at the University of Tennessee College of Veterinary Medicine. Funding sources were not involved in the study design, data analysis and interpretation, or writing of the manuscript.
The authors declare that there were no conflicts of interest.
Portable blood glucose meter
AlphaTRAK 2 blood glucose monitoring system, Zoetis, Parsippany-Troy Hills, NJ.
AlphaTRAK 2 blood glucose test strips, Zoetis, Parsippany-Troy Hills, NJ.
AlphaTRAK lancets, Zoetis, Parsippany-Troy Hills, NJ.
AlphaTRAK lancing device, Zoetis, Parsippany-Troy Hills, NJ.
SAS, version 9.4, SAS Institute Inc, Cary, NC.
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