Paratuberculosis (Johne's disease) is an important, costly enteric infection of cattle and other ruminants caused by MAP. Use of vaccination to control paratuberculosis has yielded mixed results. Studies1–3 evaluating MAP vaccines have revealed reduced prevalence of cattle with clinical disease caused by MAP infection. Although results of some studies3–5 have indicated that there is no decrease in infection rate or fecal shedding in vaccinated animals, compared with findings in unvaccinated animals, other researchers2,6 have reported reduced prevalence of infection and fecal shedding in vaccinated animals. In a study7 of the only USDA-licensed, killed vaccine against paratuberculosis, vaccination did not reduce tissue colonization with MAP in experimentally infected calves. However, the strain of organism in that vaccine (strain 18) is actually Mycobacterium avium and not MAP.8 It has been speculated that this could be a reason for vaccine failure in some situations. In the same study,7 vaccination with a heat-killed field strain of MAP did reduce tissue colonization with MAP in experimentally challenged calves. Results of preliminary studies6 in Europe of an MAP bacterin prepared from strain 316F (a bovine isolate of MAP) have suggested that this product is efficacious. The objective of the study reported here was to evaluate the effect of vaccination of calves with a killed MAP vaccine on colonization of tissues following oral exposure to the organism.
Herrolds egg yolk medium
Mycobacterium avium subsp paratuberculosis
Purified protein derivative
Time to positive results
Silirum, CZVeterianaria, Porrino, Spain.
Middlebrook 7H9 broth, BD Diagnostic Systems, Sparks, Md.
Paracheck, Biocor Animal Health, Omaha, Neb.
Johnin purified protein derivative, CSL Ltd, Parkville, VIC, Australia.
Johnin purified protein derivative, National Veterinary Services Laboratories, Ames, Iowa.
Lectin from Phytolacca americana (pokeweed), Sigma-Aldrich, St Louis, Mo.
Bovigam, Biocor Animal Health, Omaha, Neb.
BD Diagnostic Systems, Sparks, Md.
Bactec MGIT, BD Diagnostic Systems, Sparks, Md.
PROC MIXED, SAS, version 9.01, SAS Institute Inc, Cary, NC.
PROC CORR, SAS, version 9.01, SAS Institute Inc, Cary, NC.
Larsen AB, Moyle AI, Himes EM. Experimental vaccination of cattle against paratuberculosis (Johne's disease) with killed bacterial vaccines: a controlled field study. Am J Vet Res 1978;39:65–69.
Wentink GH, Bongers JH, Zeeuwen AA, et al. Incidence of paratuberculosis after vaccination against M. paratuberculosis in two infected dairy herds. Zentralbl Veterinarmed [B] 1994;41:517–522.
Larsen AB, Merkal RS, Moon HW. Evaluation of a paratuberculosis vaccine given to calves before infection. Am J Vet Res 1974;35:367–369.
Kalis CH, Hesselink JW, Barkema HW, et al. Use of long-term vaccination with a killed vaccine to prevent fecal shedding of Mycobacterium avium subsp paratuberculosis in dairy herds. Am J Vet Res 2001;62:270–274.
Munoz M, Garcia Marin JF, Garcia-Pariente C, et al. Efficacy of a killed vaccine (Silirum®) in calves challenged with MAP, in Proceedings. 8th Int Colloq Paratuberculosis 2005;208–215.
Uzonna J, Chilton P, Whitlock RH, et al. Efficacy of commercial and field-strain Mycobacterium paratuberculosis vaccinations with recombinant IL-12 in a bovine experimental infection model. Vaccine 2003;21:3101–3109.
Sweeney RW, Uzonna J, Whitlock RH, et al. Tissue predilection sites and effect of dose on Mycobacterium avium subs. paratuberculosis organism recovery in a short-term bovine experimental oral infection model. Res Vet Sci 2006;80:253–259.
Whitlock RH, Rosenberger AE. Fecal culture protocol for Mycobacterium paratuberculosis: a recommended procedure, in Proceedings. 94th Annu Meet U S Anim Health Assoc 1990;280–285.
Sweeney RW, Whitlock RH, Rosenberger AE. Mycobacterium paratuberculosis cultured from milk and supramammary lymph nodes of infected asymptomatic cows. J Clin Microbiol 1992;30:166–171.
Spangler E, Heider LE, Bech-Nielsen S, et al. Serologic enzyme-linked immunosorbent assay responses of calves vaccinated with a killed Mycobacterium paratuberculosis vaccine. Am J Vet Res 1991;52:1197–1200.