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Effect of exercise on proglycogen and macroglycogen content in skeletal muscles of pigs with the Rendement Napole mutation

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  • 1 Department of Clinical Sciences, Section for Comparative Physiology and Medicine, Faculty of Veterinary Medicine and Animal Science, Swedish University of Agricultural Sciences, S-750 07, Uppsala, Sweden.
  • | 2 Department of Clinical Sciences, Section for Comparative Physiology and Medicine, Faculty of Veterinary Medicine and Animal Science, Swedish University of Agricultural Sciences, S-750 07, Uppsala, Sweden.
  • | 3 Department of Clinical Sciences, Section for Comparative Physiology and Medicine, Faculty of Veterinary Medicine and Animal Science, Swedish University of Agricultural Sciences, S-750 07, Uppsala, Sweden.
  • | 4 Department of Medical Biochemistry and Microbiology, Uppsala Biomedical Center, Uppsala University, S-751 24, Uppsala, Sweden.

Abstract

Objective—To investigate influence of the Rendement Napole (RN) mutation on proglycogen (PG) and macroglycogen (MG) content in skeletal muscles before and after exercise and evaluate glycogen concentrations within various muscle fiber types.

Animals—5 pigs with the RN mutation and 3 noncarrier pigs.

Procedure—Pigs performed 2 exercise tests on a treadmill. In the first, pigs (mean body weight, 27 kg) ran a distance of approximately 800 m. In the second, pigs (mean body weight, 63 kg) ran until fatigued. Biopsy specimens (biceps femoris muscle) for determination of PG and MG contents were obtained before and after exercise, 24 hours after the first test, and 3 hours after the second test. Histochemical analysis was performed on specimens obtained before and after the second test.

Results—Before exercise, PG stores did not differ markedly between groups, but MG stores were twice as high in pigs with the RN mutation, compared with noncarrier pigs. The MG content decreased to a similar extent in both groups after exercise. Resynthesis of MG was greater in pigs with the RN mutation than in noncarrier pigs by 3 hours after exercise. A low glycogen content after exercise was observed in many type I and type IIA fibers and in some type IIB fibers.

Conclusions and Clinical Relevance—The RN mutation was associated with high MG stores in skeletal muscle that did not influence exercise performance. The RN mutation did not impair glycogenolysis during exercise but may induce faster resynthesis of MG after exercise. (Am J Vet Res 2005;66:1197–1201)

Abstract

Objective—To investigate influence of the Rendement Napole (RN) mutation on proglycogen (PG) and macroglycogen (MG) content in skeletal muscles before and after exercise and evaluate glycogen concentrations within various muscle fiber types.

Animals—5 pigs with the RN mutation and 3 noncarrier pigs.

Procedure—Pigs performed 2 exercise tests on a treadmill. In the first, pigs (mean body weight, 27 kg) ran a distance of approximately 800 m. In the second, pigs (mean body weight, 63 kg) ran until fatigued. Biopsy specimens (biceps femoris muscle) for determination of PG and MG contents were obtained before and after exercise, 24 hours after the first test, and 3 hours after the second test. Histochemical analysis was performed on specimens obtained before and after the second test.

Results—Before exercise, PG stores did not differ markedly between groups, but MG stores were twice as high in pigs with the RN mutation, compared with noncarrier pigs. The MG content decreased to a similar extent in both groups after exercise. Resynthesis of MG was greater in pigs with the RN mutation than in noncarrier pigs by 3 hours after exercise. A low glycogen content after exercise was observed in many type I and type IIA fibers and in some type IIB fibers.

Conclusions and Clinical Relevance—The RN mutation was associated with high MG stores in skeletal muscle that did not influence exercise performance. The RN mutation did not impair glycogenolysis during exercise but may induce faster resynthesis of MG after exercise. (Am J Vet Res 2005;66:1197–1201)