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Platelet function and association of bovine viral diarrhea virus with platelets of persistently infected cattle

Paul H. Walz DVM, PhD1, Daniel L. Grooms DVM, PhD2, Thomas G. Bell DVM, PhD3, Lana Kaiser MD, DVM4, John C. Baker DVM, PhD5, and Kenny V. Brock DVM, PhD6
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  • 1 Departments of Clinical Sciences and Pathobiology, College of Veterinary Medicine, Auburn University, Auburn, AL 36849-5519.
  • | 2 Department of Large Animal Clinical Sciences, Michigan State University, East Lansing, MI 48824-1314.
  • | 3 Department of Pathobiology and Diagnostic Investigations, College of Veterinary Medicine, Auburn University, Auburn, AL 36849-5519.
  • | 4 College of Veterinary Medicine, and the Department of Medicine, Hematology/Oncology, College of Human Medicine, Michigan State University, East Lansing, MI 48824-1314.
  • | 5 Department of Large Animal Clinical Sciences, Michigan State University, East Lansing, MI 48824-1314.
  • | 6 Department of Pathobiology, College of Veterinary Medicine, Auburn University, Auburn, AL 36849-5519.

Abstract

Objective—To determine whether viral involvement with platelets obtained from cattle persistently infected (PI) with bovine viral diarrhea virus (BVDV) is associated with altered platelet function or decreased platelet counts.

Sample Population—Platelets obtained from 8 cattle PI with BVDV and 6 age-, sex-, and breed-matched uninfected control cattle.

Procedure—Manual platelet counts were determined, and platelet function was assessed through optical aggregometry by use of the aggregation agonists ADP and platelet-activating factor. Identification of BVDV in serum and preparations of purified platelets was determined by use of virus isolation tests.

Results—No significant difference in platelet counts was detected between cattle PI with BVDV and control cattle. In response to the aggregation agonists, maximum aggregation percentage and slope of the aggregation curve were not significantly different between cattle PI with BVDV and control cattle. We isolated BVDV from serum of all PI cattle and from purified platelets of 6 of 8 PI cattle, but BVDV was not isolated from serum or platelets of control cattle.

Conclusions and Clinical Relevance—Isolation of BVDV from platelets in the peripheral circulation of cattle immunotolerant to BVDV does not result in altered platelet function or decreases in platelet counts. (Am J Vet Res 2005;66:1738–1742)

Abstract

Objective—To determine whether viral involvement with platelets obtained from cattle persistently infected (PI) with bovine viral diarrhea virus (BVDV) is associated with altered platelet function or decreased platelet counts.

Sample Population—Platelets obtained from 8 cattle PI with BVDV and 6 age-, sex-, and breed-matched uninfected control cattle.

Procedure—Manual platelet counts were determined, and platelet function was assessed through optical aggregometry by use of the aggregation agonists ADP and platelet-activating factor. Identification of BVDV in serum and preparations of purified platelets was determined by use of virus isolation tests.

Results—No significant difference in platelet counts was detected between cattle PI with BVDV and control cattle. In response to the aggregation agonists, maximum aggregation percentage and slope of the aggregation curve were not significantly different between cattle PI with BVDV and control cattle. We isolated BVDV from serum of all PI cattle and from purified platelets of 6 of 8 PI cattle, but BVDV was not isolated from serum or platelets of control cattle.

Conclusions and Clinical Relevance—Isolation of BVDV from platelets in the peripheral circulation of cattle immunotolerant to BVDV does not result in altered platelet function or decreases in platelet counts. (Am J Vet Res 2005;66:1738–1742)