Quantitation of house dust mite allergens (Der f 1 and group 2) on the skin and hair of dogs

Amy J. Randall Carolina Veterinary Specialists, 501 Nicholas Rd, Greensboro, NC 27409.

Search for other papers by Amy J. Randall in
Current site
Google Scholar
PubMed
Close
 DVM, MS
,
Andrew Hillier Department of Veterinary Clinical Sciences, College of Veterinary Medicine, The Ohio State University, Columbus, OH 43210.

Search for other papers by Andrew Hillier in
Current site
Google Scholar
PubMed
Close
 BVSc
,
Lynette K. Cole Department of Veterinary Clinical Sciences, College of Veterinary Medicine, The Ohio State University, Columbus, OH 43210.

Search for other papers by Lynette K. Cole in
Current site
Google Scholar
PubMed
Close
 DVM, MS
,
Kenneth W. Kwochka DVM Pharmaceuticals, 50 NW 176 St, Miami, FL 33169.

Search for other papers by Kenneth W. Kwochka in
Current site
Google Scholar
PubMed
Close
 DVM
,
Glen Needham Acarology Laboratory, Department of Entomology, College of Biological Sciences, The Ohio State University, Columbus, OH 43210.

Search for other papers by Glen Needham in
Current site
Google Scholar
PubMed
Close
 PhD
, and
Donald L. Wassom Heska AG, 16 Grands Pl CH-1700, Fribourg, Switzerland.

Search for other papers by Donald L. Wassom in
Current site
Google Scholar
PubMed
Close
 PhD

Abstract

Objective—To determine the concentration of house dust mite (HDM) allergens, Der f 1 and group 2, on the skin and hair of dogs and whether associations exist between the presence of Der f 1 and group 2 allergens on the skin and hair of dogs and household and dog characteristics.

Animals—63 pet dogs from 50 homes.

Procedure—Dogs were weighed and body surface area in square meters was determined. Skin and hair samples were obtained by vacuuming dogs. Collected dust was analyzed by use of standard ELISA techniques.

Results—HDM allergen was detected in 21 of 59 skin and hair samples. Presence of group 2 allergen on skin and hair of dogs was significantly associated with long hair, compared with short or medium length hair. Median house dust sample concentrations of Der f 1 and group 2 allergens were high in homes with dogs that had skin and hair samples that were positive for Der f 1 and group 2 allergens. Dogs with skin and hair samples that were positive for Der f 1 and group 2 allergens resided in homes with a high number of house dust samples that were positive for Der f 1, group 2, or both allergens and in homes with a mean house dust sample allergen concentration of ≥ 2 µg/g of dust.

Conclusions and Clinical Relevance—Associations exist between environmental HDM allergen concentrations and HDM allergens on the skin and hair samples of dogs. Environmental allergen load is a major factor in accumulation of allergens on the skin and hair of dogs. (Am J Vet Res 2005;66:143–149)

Abstract

Objective—To determine the concentration of house dust mite (HDM) allergens, Der f 1 and group 2, on the skin and hair of dogs and whether associations exist between the presence of Der f 1 and group 2 allergens on the skin and hair of dogs and household and dog characteristics.

Animals—63 pet dogs from 50 homes.

Procedure—Dogs were weighed and body surface area in square meters was determined. Skin and hair samples were obtained by vacuuming dogs. Collected dust was analyzed by use of standard ELISA techniques.

Results—HDM allergen was detected in 21 of 59 skin and hair samples. Presence of group 2 allergen on skin and hair of dogs was significantly associated with long hair, compared with short or medium length hair. Median house dust sample concentrations of Der f 1 and group 2 allergens were high in homes with dogs that had skin and hair samples that were positive for Der f 1 and group 2 allergens. Dogs with skin and hair samples that were positive for Der f 1 and group 2 allergens resided in homes with a high number of house dust samples that were positive for Der f 1, group 2, or both allergens and in homes with a mean house dust sample allergen concentration of ≥ 2 µg/g of dust.

Conclusions and Clinical Relevance—Associations exist between environmental HDM allergen concentrations and HDM allergens on the skin and hair samples of dogs. Environmental allergen load is a major factor in accumulation of allergens on the skin and hair of dogs. (Am J Vet Res 2005;66:143–149)

All Time Past Year Past 30 Days
Abstract Views 22 0 0
Full Text Views 723 613 221
PDF Downloads 85 62 5
Advertisement