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Influence of dietary long-chain n-3 fatty acids from Menhaden fish oil on plasma concentrations of α-tocopherol in geriatric Beagles

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  • 1 Department of Biomedical Sciences, College of Veterinary Medicine, Oregon State University, Corvallis, OR 97331-4802.
  • | 2 Department of Biomedical Sciences, College of Veterinary Medicine, Oregon State University, Corvallis, OR 97331-4802.
  • | 3 Department of Biomedical Sciences, College of Veterinary Medicine, Oregon State University, Corvallis, OR 97331-4802.
  • | 4 Science and Technology Center, Hill's Pet Nutrition Inc, 1035 NE 43rd Street, Topeka, KS 66617-1587.
  • | 5 Department of Nutrition and Foodservice Systems, School of Human Environmental Sciences, University of North Carolina, Greensboro, NC 27402-6170.

Abstract

Objective—To determine effects of dietary n-3 fatty acids from Menhaden fish oil on plasma α-tocopherol concentrations in Beagles.

Animals—32 female Beagles.

Procedure—For 82 days, dogs were fed diets that contained 1 of 2 ratios of n-6:n-3 fatty acids (40:1 [low n-3] and 1.4:1 [high n-3]) and 1 of 3 concentrations of all- rac- α-tocopheryl acetate (low, 17 mg/kg of diet; medium, 101 mg/kg; and high, 447 mg/kg) in a 2 X 3 factorial study.

Results—Diets high in n-3 fatty acids significantly increased total content of n-3 fatty acids in plasma (17.0 g/100 g of fatty acids), compared with low n-3 diets (2.02 g/100 g of fatty acids). Mean ± SEM plasma concentration of cholesterol was significantly lower in dogs consuming high n-3 diets (4.59 ± 0.48 mmol/L), compared with dogs consuming low n-3 diets (5.71 ± 0.48 mmol/L). A significant interaction existed between the ratio for n-6 and n-3 fatty acids and amount of α-tocopheryl acetate in the diet (plasma α-tocopherol concentration expressed on a molar basis), because the plasma concentration of α-tocopherol was higher in dogs consuming low n-3 diets, compared with those consuming high n-3 diets, at the 2 higher amounts of dietary α-tocopheryl acetate. Plasma α-tocopherol concentration expressed relative to total lipid content did not reveal effects of dietary n-3 fatty acids on concentration of α-tocopherol.

Conclusion and Clinical Relevance—Plasma α-tocopherol concentration is not dependent on dietary ratio of n-6 and n-3 fatty acids when α-tocopherol concentration is expressed relative to the total lipid content of plasma. (Am J Vet Res 2002;63:104–110)

Abstract

Objective—To determine effects of dietary n-3 fatty acids from Menhaden fish oil on plasma α-tocopherol concentrations in Beagles.

Animals—32 female Beagles.

Procedure—For 82 days, dogs were fed diets that contained 1 of 2 ratios of n-6:n-3 fatty acids (40:1 [low n-3] and 1.4:1 [high n-3]) and 1 of 3 concentrations of all- rac- α-tocopheryl acetate (low, 17 mg/kg of diet; medium, 101 mg/kg; and high, 447 mg/kg) in a 2 X 3 factorial study.

Results—Diets high in n-3 fatty acids significantly increased total content of n-3 fatty acids in plasma (17.0 g/100 g of fatty acids), compared with low n-3 diets (2.02 g/100 g of fatty acids). Mean ± SEM plasma concentration of cholesterol was significantly lower in dogs consuming high n-3 diets (4.59 ± 0.48 mmol/L), compared with dogs consuming low n-3 diets (5.71 ± 0.48 mmol/L). A significant interaction existed between the ratio for n-6 and n-3 fatty acids and amount of α-tocopheryl acetate in the diet (plasma α-tocopherol concentration expressed on a molar basis), because the plasma concentration of α-tocopherol was higher in dogs consuming low n-3 diets, compared with those consuming high n-3 diets, at the 2 higher amounts of dietary α-tocopheryl acetate. Plasma α-tocopherol concentration expressed relative to total lipid content did not reveal effects of dietary n-3 fatty acids on concentration of α-tocopherol.

Conclusion and Clinical Relevance—Plasma α-tocopherol concentration is not dependent on dietary ratio of n-6 and n-3 fatty acids when α-tocopherol concentration is expressed relative to the total lipid content of plasma. (Am J Vet Res 2002;63:104–110)