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Oral vaccination of sexually mature pigs with Brucella abortus vaccine strain RB51

Matthew D. EdmondsDepartments of Veterinary Microbiology and Parasitology, School of Veterinary Medicine, Louisiana State University, Baton Rouge, LA 70803.

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Luis E. SamartinoInstituto de Patobiologia, Centro de Ciencias Veterinarias y Agronomicas, Buenos Aires, Argentina 1708.

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Phillip G. HoytDepartment of Clinical Sciences, School of Veterinary Medicine, Louisiana State University, Baton Rouge, LA 70803.

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Sue D. HagiusDepartment of Veterinary Science, Louisiana State University AgCenter, Louisiana State University, Baton Rouge, LA 70803.

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Joel V. WalkerDepartment of Veterinary Science, Louisiana State University AgCenter, Louisiana State University, Baton Rouge, LA 70803.

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Fred M. EnrightDepartment of Veterinary Science, Louisiana State University AgCenter, Louisiana State University, Baton Rouge, LA 70803.

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Gerhardt G. SchurigDepartment of Biomedical Sciences and Pathobiology, Virginia-Maryland Regional College of Veterinary Medicine, Virginia Polytechnic Institute and State University, Blacksburg, VA 24061.

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Philip ElzerDepartments of Veterinary Microbiology and Parasitology, School of Veterinary Medicine, Louisiana State University, Baton Rouge, LA 70803.
Department of Veterinary Science, Louisiana State University AgCenter, Louisiana State University, Baton Rouge, LA 70803.

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Abstract

Objective—To develop a novel oral vaccine delivery system for swine, using the rough vaccine strain of Brucella abortus.

Animals—56 crossbred pigs from a brucellosis-free facility.

Procedure—In 3 separate experiments, pigs were orally vaccinated with doses of 1 × 109 to > 1 × 1011 CFU of strain RB51 vaccine. The vaccine was placed directly on the normal corn ration, placed inside a whole pecan, or mixed with cracked pecans and corn.

Results—Oral vaccination of pigs with vaccine strain RB51 resulted in a humoral immune response to strain RB51 and short-term colonization of the regional lymph nodes.

Conclusions and Clinical Relevance—A viscous liquid such as Karo corn syrup in association with pecans that scarify the oral mucosa are necessary when placing the live vaccine directly onto corn or other food rations. Doses of > 1 × 1011 CFU of RB51 organisms/pig in this mixture ensures 100% colonization of regional lymph nodes via the oral route. This method may allow an efficient and economical means to vaccinate feral swine for brucellosis. (Am J Vet Res 2001;62:1328–1331)

Abstract

Objective—To develop a novel oral vaccine delivery system for swine, using the rough vaccine strain of Brucella abortus.

Animals—56 crossbred pigs from a brucellosis-free facility.

Procedure—In 3 separate experiments, pigs were orally vaccinated with doses of 1 × 109 to > 1 × 1011 CFU of strain RB51 vaccine. The vaccine was placed directly on the normal corn ration, placed inside a whole pecan, or mixed with cracked pecans and corn.

Results—Oral vaccination of pigs with vaccine strain RB51 resulted in a humoral immune response to strain RB51 and short-term colonization of the regional lymph nodes.

Conclusions and Clinical Relevance—A viscous liquid such as Karo corn syrup in association with pecans that scarify the oral mucosa are necessary when placing the live vaccine directly onto corn or other food rations. Doses of > 1 × 1011 CFU of RB51 organisms/pig in this mixture ensures 100% colonization of regional lymph nodes via the oral route. This method may allow an efficient and economical means to vaccinate feral swine for brucellosis. (Am J Vet Res 2001;62:1328–1331)