Advertisement

Biochemical characterization of cartilage affected by osteochondritis dissecans in the humeral head of dogs

James L. TomlinsonComparative Orthopaedic Laboratory, College of Veterinary Medicine, University of Missouri-Columbia, Columbia, MO 65211.

Search for other papers by James L. Tomlinson in
Current site
Google Scholar
PubMed
Close
 DVM, MVSc
,
James L. CookComparative Orthopaedic Laboratory, College of Veterinary Medicine, University of Missouri-Columbia, Columbia, MO 65211.

Search for other papers by James L. Cook in
Current site
Google Scholar
PubMed
Close
 DVM, PhD
,
Keiichi KurokiComparative Orthopaedic Laboratory, College of Veterinary Medicine, University of Missouri-Columbia, Columbia, MO 65211.

Search for other papers by Keiichi Kuroki in
Current site
Google Scholar
PubMed
Close
 DVM
,
John M. KreegerComparative Orthopaedic Laboratory, College of Veterinary Medicine, University of Missouri-Columbia, Columbia, MO 65211.

Search for other papers by John M. Kreeger in
Current site
Google Scholar
PubMed
Close
 DVM, PhD
, and
Mark A. AndersonVeterinary Surgical Services, 1520 S Brentwood Blvd, St. Louis, MO 63144.

Search for other papers by Mark A. Anderson in
Current site
Google Scholar
PubMed
Close
 DVM

Abstract

Objective—To determine glycosaminoglycan (GAG) concentration and immunohistochemical staining characteristics of type-I, -II, and -X collagen from cartilage affected by osteochondritis dissecans (OCD) in dogs.

Animals—31 dogs with OCD and 11 clinically normal purpose-bred dogs.

Procedure—Cartilage samples were evaluated microscopically, and GAG content was determined. Immunohistochemical staining was performed for type-I, -II, and -X collagen. Sections were subjectively evaluated for location and intensity of staining.

Results—Cartilage affected by OCD had a variety of pathologic changes and significantly lower GAG concentrations than did normal cartilage. Normal cartilage had no detectable type-I collagen. For dogs < 9 months of age, cartilage affected by OCD had significantly more type-I collagen but significantly less type- X collagen than did control cartilage. For dogs > 12 months of age, cartilage affected by OCD contained significantly more type-I collagen than did control cartilage. There was a significant negative correlation between immunoreactivity of type-I collagen and that of type-II and -X collagen. A significant positive correlation was found between immunoreactivity of type-II and -X collagen.

Conclusions and Clinical Relevance—Cartilage affected by OCD contains less GAG, more type-I collagen, and less type-X collagen, compared with normal cartilage. A direct correlation between these changes and the etiopathogenesis of OCD was not established. (Am J Vet Res 2001;62:876–881)

Abstract

Objective—To determine glycosaminoglycan (GAG) concentration and immunohistochemical staining characteristics of type-I, -II, and -X collagen from cartilage affected by osteochondritis dissecans (OCD) in dogs.

Animals—31 dogs with OCD and 11 clinically normal purpose-bred dogs.

Procedure—Cartilage samples were evaluated microscopically, and GAG content was determined. Immunohistochemical staining was performed for type-I, -II, and -X collagen. Sections were subjectively evaluated for location and intensity of staining.

Results—Cartilage affected by OCD had a variety of pathologic changes and significantly lower GAG concentrations than did normal cartilage. Normal cartilage had no detectable type-I collagen. For dogs < 9 months of age, cartilage affected by OCD had significantly more type-I collagen but significantly less type- X collagen than did control cartilage. For dogs > 12 months of age, cartilage affected by OCD contained significantly more type-I collagen than did control cartilage. There was a significant negative correlation between immunoreactivity of type-I collagen and that of type-II and -X collagen. A significant positive correlation was found between immunoreactivity of type-II and -X collagen.

Conclusions and Clinical Relevance—Cartilage affected by OCD contains less GAG, more type-I collagen, and less type-X collagen, compared with normal cartilage. A direct correlation between these changes and the etiopathogenesis of OCD was not established. (Am J Vet Res 2001;62:876–881)