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Molecular epidemiologic features and antimicrobial susceptibility profiles of various ribotypes of Pseudomonas aeruginosa isolated from humans and ruminants

Ariel L. RivasDepartment of Population Medicine and Diagnostic Sciences, Cornell University, Ithaca, NY 14850.

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Mary BodisCollege of Veterinary Medicine, and the Laboratory for Molecular Typing, Department of Food Science, College of Agriculture and Life Sciences, Cornell University, Ithaca, NY 14850.

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James L. BruceDuPont Qualicon, 3531 Silverside Rd, Wilmington, DE 19810.

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Kevin L. AndersonDepartment of Farm Animal Health and Resource Management, College of Veterinary Medicine, North Carolina State University, Raleigh, NC 27606.

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Renate F. KleinDepartment of Pathology, The New York Hospital-Cornell Medical Center, New York, NY 10021.

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Rubén N. GonzálezDepartment of Population Medicine and Diagnostic Sciences, Cornell University, Ithaca, NY 14850.

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Fred W. QuimbyDepartment of Biomedical Sciences, Cornell University, Ithaca, NY 14850.

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Carl A. BattCollege of Veterinary Medicine, and the Laboratory for Molecular Typing, Department of Food Science, College of Agriculture and Life Sciences, Cornell University, Ithaca, NY 14850.

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Donald H. LeinDepartment of Population Medicine and Diagnostic Sciences, Cornell University, Ithaca, NY 14850.

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Abstract

Objectives—To assess automated ribotyping for characterization of Pseudomonas aeruginosa isolates and to identify their type prevalence and geographic distribution.

Sample Population—39 human and 56 ruminant P aeruginosa isolates.

Procedures—Isolates were identified by use of bacteriologic techniques and automated PvuII-based ribotyping. Susceptibility to antimicrobials was tested in vitro. Data were analyzed for index of discrimination; prevalence ratio; geographic distribution of ribotypes found only in humans, only in cows, or only in goats (single-host ribotypes); and geographic distribution of ribotypes found in humans and ruminants (multihost ribotypes).

Results—All isolates were typeable (45 ribotypes, 35 single-host ribotypes). Ribotyping index of discrimination was 0.976. More isolates (45.3%) than expected yielded multihost ribotypes (22% of all ribotypes). Although 8.6% of single-host ribotypes were found in 4 or more isolates, 60% of multihost ribotypes were found in 4 or more isolates. Ninety percent of multihost ribotypes were isolated from different geographic areas, whereas 3.0% of singlehost ribotypes were isolated from different geographic areas. All ruminant isolates were susceptible to gentamicin and polymyxin B. In contrast, antibiogram profiles differed for human isolates from different geographic areas. Susceptibility to antimicrobials differentiated 6 isolates not distinguished by ribotyping.

Conclusions and Clinical Relevance—Automated ribotyping with PvuII discriminated more isolates than in vitro antimicrobial susceptibility. In combination, both tests provided more information than either test alone. Given the greater prevalence and geographic distribution of multihost ribotypes, immunocompromised humans and lactating ruminants may have a greater risk for disease if exposed to multihost P aeruginosa ribotypes, compared with single-host ribotypes. (Am J Vet Res 2001;62:864–870)

Abstract

Objectives—To assess automated ribotyping for characterization of Pseudomonas aeruginosa isolates and to identify their type prevalence and geographic distribution.

Sample Population—39 human and 56 ruminant P aeruginosa isolates.

Procedures—Isolates were identified by use of bacteriologic techniques and automated PvuII-based ribotyping. Susceptibility to antimicrobials was tested in vitro. Data were analyzed for index of discrimination; prevalence ratio; geographic distribution of ribotypes found only in humans, only in cows, or only in goats (single-host ribotypes); and geographic distribution of ribotypes found in humans and ruminants (multihost ribotypes).

Results—All isolates were typeable (45 ribotypes, 35 single-host ribotypes). Ribotyping index of discrimination was 0.976. More isolates (45.3%) than expected yielded multihost ribotypes (22% of all ribotypes). Although 8.6% of single-host ribotypes were found in 4 or more isolates, 60% of multihost ribotypes were found in 4 or more isolates. Ninety percent of multihost ribotypes were isolated from different geographic areas, whereas 3.0% of singlehost ribotypes were isolated from different geographic areas. All ruminant isolates were susceptible to gentamicin and polymyxin B. In contrast, antibiogram profiles differed for human isolates from different geographic areas. Susceptibility to antimicrobials differentiated 6 isolates not distinguished by ribotyping.

Conclusions and Clinical Relevance—Automated ribotyping with PvuII discriminated more isolates than in vitro antimicrobial susceptibility. In combination, both tests provided more information than either test alone. Given the greater prevalence and geographic distribution of multihost ribotypes, immunocompromised humans and lactating ruminants may have a greater risk for disease if exposed to multihost P aeruginosa ribotypes, compared with single-host ribotypes. (Am J Vet Res 2001;62:864–870)