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Microvascular development and growth of uterine tissue during the estrous cycle in mares

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  • 1 Faculdade de Medicina Veterinária, Centro de Investigação Interdisciplinar em Sanidade Animal, Rua Prof. Cid dos Santos, Polo Universitário Alto da Ajuda, 1300-477 Lisbon, Portugal.
  • | 2 Faculdade de Medicina Veterinária, Centro de Investigação Interdisciplinar em Sanidade Animal, Rua Prof. Cid dos Santos, Polo Universitário Alto da Ajuda, 1300-477 Lisbon, Portugal.
  • | 3 Faculdade de Medicina Veterinária, Centro de Investigação Interdisciplinar em Sanidade Animal, Rua Prof. Cid dos Santos, Polo Universitário Alto da Ajuda, 1300-477 Lisbon, Portugal.
  • | 4 Faculdade de Medicina Veterinária, Centro de Investigação Interdisciplinar em Sanidade Animal, Rua Prof. Cid dos Santos, Polo Universitário Alto da Ajuda, 1300-477 Lisbon, Portugal.

Abstract

Objective—To document uterine growth and microvascular development in the endometrium of uteri with differing degrees of fibrosis as well as uterine growth throughout the estrous cycle of mares.

Animals—30 mares.

Procedure—Uterine tissue was obtained during the breeding season from a slaughter facility. Stage of estrous cycle of the mares was assessed on the basis of ovarian structures and plasma progesterone concentrations. Endometrium was characterized by use of light microscopy, and blood vessel walls were marked by histochemical techniques. Microvascular development was evaluated by a computerized image analysis system. Growth of uterine tissue was based on cellular content of DNA and RNA, RNA:DNA, and protein:DNA.

Results—Significant differences in vascular density were not observed in the endometrium of uteri obtained from mares euthanatized during the follicular or luteal phase of the estrous cycle, regardless of whether endometrial classification of degree of fibrosis was considered. There was a 3-fold increase in amount of DNA and RNA of endometrial cells in the follicular phase when compared to myometrium. Hypertrophy of endometrial tissue during the luteal phase was reflected by a significant increase in cell protein content and protein:DNA.

Conclusion and Clinical Relevance—Endometrial growth of vascular tissues during the estrous cycle may be coordinated with development of nonvascular tissue. Estrogen and progesterone may play a role in regulation of uterine growth and angiogenesis. (Am J Vet Res 2001;62:526–530)

Abstract

Objective—To document uterine growth and microvascular development in the endometrium of uteri with differing degrees of fibrosis as well as uterine growth throughout the estrous cycle of mares.

Animals—30 mares.

Procedure—Uterine tissue was obtained during the breeding season from a slaughter facility. Stage of estrous cycle of the mares was assessed on the basis of ovarian structures and plasma progesterone concentrations. Endometrium was characterized by use of light microscopy, and blood vessel walls were marked by histochemical techniques. Microvascular development was evaluated by a computerized image analysis system. Growth of uterine tissue was based on cellular content of DNA and RNA, RNA:DNA, and protein:DNA.

Results—Significant differences in vascular density were not observed in the endometrium of uteri obtained from mares euthanatized during the follicular or luteal phase of the estrous cycle, regardless of whether endometrial classification of degree of fibrosis was considered. There was a 3-fold increase in amount of DNA and RNA of endometrial cells in the follicular phase when compared to myometrium. Hypertrophy of endometrial tissue during the luteal phase was reflected by a significant increase in cell protein content and protein:DNA.

Conclusion and Clinical Relevance—Endometrial growth of vascular tissues during the estrous cycle may be coordinated with development of nonvascular tissue. Estrogen and progesterone may play a role in regulation of uterine growth and angiogenesis. (Am J Vet Res 2001;62:526–530)