Serum lipid peroxide and α-tocopherol concentrations and superoxide dismutase activity in captive bottle-nosed dolphins

Masahiko Kasamatsu Department of Animal Health, School of Veterinary Medicine, Rakuno Gakuen University, Ebetsu, Hokkaido 069-8501, Japan.

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Masatoshi Tsunokawa Otaru Aquarium, Otaru, Hokkaido 047-0047, Japan.

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Michihiro Taki Suma Aqualife Park, Kobe, Hyogo 654-0049, Japan.

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Hidetoshi Higuchi Department of Animal Health, School of Veterinary Medicine, Rakuno Gakuen University, Ebetsu, Hokkaido 069-8501, Japan.

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Hajime Nagahata Department of Animal Health, School of Veterinary Medicine, Rakuno Gakuen University, Ebetsu, Hokkaido 069-8501, Japan.

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Abstract

Objective—To evaluate serum lipid peroxide (LPO) and α-tocopherol concentrations and superoxide dismutase (SOD) activity in captive bottle-nosed dolphins and to evaluate effects of storage on production of LPO in various marine fish.

Animals—16 bottle-nosed dolphins.

Procedure—8 dolphins (group A) were fed chub mackerel and herring (high fat) and arabesque greenling and banded blue-sprat (low fat); the other 8 dolphins (group B) were fed chub mackerel and Pacific saury (high fat) and shishamo smelt and Japanese horse mackerel (low fat). Each group had been on these respective diets for 3 years. Serum LPO and α-tocopherol concentrations, serum SOD activity, and superoxide production by neutrophils were measured. All types of marine fish were frozen at –20 C for 6 months, and concentrations of LPO were measured at various time points.

Results—Serum LPO concentrations in group-A dolphins were significantly higher than those in group B. Serum α-tocopherol concentrations and SOD activity in group A were significantly lower than those in group B. A significant negative correlation was found between serum LPO and α-tocopherol concentrations in all 16 dolphins. The LPO concentrations in mackerel and herring fed to group-A dolphins were higher than those of other fish. Concentrations of LPO in herring stored for 3 and 6 months at –20 C were higher than those in herring before freezing and in herring stored for 1 month.

Conclusions and Clinical Relevance—Serum LPO and α-tocopherol concentrations in captive bottlenosed dolphins may be strongly influenced by high amounts of polyunsaturated fatty acid and LPO found in marine fatty fishes. High concentrations of serum LPO, as found in group-A dolphins, were associated with decreased antioxidative states. Monitoring of serum LPO and α-tocopherol concentrations and serum SOD activity may be useful for the management of captive marine mammals. (Am J Vet Res 2001;62:1952–1956)

Abstract

Objective—To evaluate serum lipid peroxide (LPO) and α-tocopherol concentrations and superoxide dismutase (SOD) activity in captive bottle-nosed dolphins and to evaluate effects of storage on production of LPO in various marine fish.

Animals—16 bottle-nosed dolphins.

Procedure—8 dolphins (group A) were fed chub mackerel and herring (high fat) and arabesque greenling and banded blue-sprat (low fat); the other 8 dolphins (group B) were fed chub mackerel and Pacific saury (high fat) and shishamo smelt and Japanese horse mackerel (low fat). Each group had been on these respective diets for 3 years. Serum LPO and α-tocopherol concentrations, serum SOD activity, and superoxide production by neutrophils were measured. All types of marine fish were frozen at –20 C for 6 months, and concentrations of LPO were measured at various time points.

Results—Serum LPO concentrations in group-A dolphins were significantly higher than those in group B. Serum α-tocopherol concentrations and SOD activity in group A were significantly lower than those in group B. A significant negative correlation was found between serum LPO and α-tocopherol concentrations in all 16 dolphins. The LPO concentrations in mackerel and herring fed to group-A dolphins were higher than those of other fish. Concentrations of LPO in herring stored for 3 and 6 months at –20 C were higher than those in herring before freezing and in herring stored for 1 month.

Conclusions and Clinical Relevance—Serum LPO and α-tocopherol concentrations in captive bottlenosed dolphins may be strongly influenced by high amounts of polyunsaturated fatty acid and LPO found in marine fatty fishes. High concentrations of serum LPO, as found in group-A dolphins, were associated with decreased antioxidative states. Monitoring of serum LPO and α-tocopherol concentrations and serum SOD activity may be useful for the management of captive marine mammals. (Am J Vet Res 2001;62:1952–1956)

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