Pathologic findings and association of Mycobacterium bovis infection with the bovine NRAMP1 gene in cattle from herds with naturally occurring tuberculosis

Robert Barthel Departments of Veterinary Pathobiology, College of Medicine, Texas A&M University, College Station, TX 77843-4467.

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Jorge A. Piedrahita Veterinary Anatomy and Public Health, College of Medicine, Texas A&M University, College Station, TX 77843-4467.

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David N. McMurray College of Veterinary Medicine, and the Department of Medical Microbiology and Immunology, College of Medicine, Texas A&M University, College Station, TX 77843-4467.

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Janet Payeur National Veterinary Services Laboratories, National Vererinary Services Laboratories, 1800 Dayton Ave, Ames, IA 50011.

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Daniel Baca Texas Animal Health Commission, Texas Animal Health Commission, 2105 Kramer Ln, Austin, TX 78758.

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Francisco Suárez Güemes Departamento de Microbiología e Inmunología, Facultad de Medicina Veterinaria y Zootecnia, Universidad Nacional Autónoma de México, Coyoacan DF, México 04510.

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Veera S. Perumaalla Departments of Veterinary Pathobiology, College of Medicine, Texas A&M University, College Station, TX 77843-4467.

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Thomas A. Ficht Departments of Veterinary Pathobiology, College of Medicine, Texas A&M University, College Station, TX 77843-4467.

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Joe W. Templeton Departments of Veterinary Pathobiology, College of Medicine, Texas A&M University, College Station, TX 77843-4467.

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L. Garry Adams Departments of Veterinary Pathobiology, College of Medicine, Texas A&M University, College Station, TX 77843-4467.

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Abstract

Objective—To determine necropsy and Mycobacterium bovis culture results in cattle from herds with tuberculosis, the role of the bovine NRAMP1 gene in resistance and susceptibility to infection with M bovis, and the association between magnitude of the tuberculous lesions and various types of M bovis isolates.

Animals—61 cattle from herds with tuberculosis in Texas and Mexico.

Procedure—61 cattle were evaluated by necropsy; 59 had positive and 2 had negative caudal fold tuberculin intradermal test (CFT) results. Thirty-three cattle with positive CFT results were genotyped to evaluate polymorphism of the 3' untranslated region of the bovine NRAMP1 gene, using single-stranded conformational analysis, 9 were resistant to M bovis with no tuberculous lesions and negative M bovis culture results, and 24 were susceptible with tuberculous lesions and positive M bovis culture results. Isolates of M bovis were analyzed by restriction fragment length polymorphism (RFLP) on the basis of IS6110 sequences and direct-repeat fingerprinting patterns.

Results—21 (35.6%; 21/59) cattle with positive CFT results had tuberculous lesions or positive culture results; in addition, 1 of 2 cattle with negative CFT results had tuberculous lesions and positive culture results. Tuberculous lesions were most common in the thorax (35/63; 55.5%) and lymphoid tissues of the head (10/63; 15.9%). Tuberculous lesions varied from 1 to 11/animal; 8 of 21 (38.1%) had solitary lesions. Associations were not found between resistance or susceptibility to infection with M bovis and polymorphism in the NRAMP1 gene or between the magnitude of the lesions and various RFLP types of M bovis isolates.

Conclusions and Clinical Relevance—The NRAMP1 gene does not determine resistance and susceptibility to infection with M bovis in cattle. (Am J Vet Res 2000;61:1140–1144)

Abstract

Objective—To determine necropsy and Mycobacterium bovis culture results in cattle from herds with tuberculosis, the role of the bovine NRAMP1 gene in resistance and susceptibility to infection with M bovis, and the association between magnitude of the tuberculous lesions and various types of M bovis isolates.

Animals—61 cattle from herds with tuberculosis in Texas and Mexico.

Procedure—61 cattle were evaluated by necropsy; 59 had positive and 2 had negative caudal fold tuberculin intradermal test (CFT) results. Thirty-three cattle with positive CFT results were genotyped to evaluate polymorphism of the 3' untranslated region of the bovine NRAMP1 gene, using single-stranded conformational analysis, 9 were resistant to M bovis with no tuberculous lesions and negative M bovis culture results, and 24 were susceptible with tuberculous lesions and positive M bovis culture results. Isolates of M bovis were analyzed by restriction fragment length polymorphism (RFLP) on the basis of IS6110 sequences and direct-repeat fingerprinting patterns.

Results—21 (35.6%; 21/59) cattle with positive CFT results had tuberculous lesions or positive culture results; in addition, 1 of 2 cattle with negative CFT results had tuberculous lesions and positive culture results. Tuberculous lesions were most common in the thorax (35/63; 55.5%) and lymphoid tissues of the head (10/63; 15.9%). Tuberculous lesions varied from 1 to 11/animal; 8 of 21 (38.1%) had solitary lesions. Associations were not found between resistance or susceptibility to infection with M bovis and polymorphism in the NRAMP1 gene or between the magnitude of the lesions and various RFLP types of M bovis isolates.

Conclusions and Clinical Relevance—The NRAMP1 gene does not determine resistance and susceptibility to infection with M bovis in cattle. (Am J Vet Res 2000;61:1140–1144)

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