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Use of a nonionic detergent (Triton WR 1339) in healthy cats to assess hepatic secretion of triglyceride

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  • 1 Department of Biomedical Sciences, College of Veterinary Medicine, Oregon State University, Corvallis, OR 97331-4802.
  • | 2 Department of Biomedical Sciences, College of Veterinary Medicine, Oregon State University, Corvallis, OR 97331-4802.
  • | 3 Department of Biomedical Sciences, College of Veterinary Medicine, Oregon State University, Corvallis, OR 97331-4802.
  • | 4 Andreasen's present address is Department of Veterinary Pathology, College of Veterinary Medicine, Iowa State University, Ames, IA 50011- 1250.
  • | 5 Department of Nutrition and Food Management, College of Home Economics and Education, Oregon State University, Corvallis, OR 97331-4802.
  • | 6 present address is Department of Nutrition and Foodservice Systems, School of Human Environmental Sciences, University of North Carolina, Greensboro, NC 27402-6170.

Abstract

Objective—To determine whether a nonionic detergent (Triton WR 1339) can be used in cats to assess hepatic secretion of triglyceride.

Animals—28 healthy cats.

Procedure—Triton WR 1339 was administered IV according to the following schedule: 5, 50, 150, and 250 mg/kg of body weight. Control cats did not receive an injection or received 0.9% NaCl or PBS solutions at the same osmolarity and volume as the 250 mg/kg group. Blood samples were collected throughout the 48-hour period after administration for determination of triglyceride and cholesterol concentrations and for RBC morphology and osmotic fragility studies.

Results—Administration of Triton WR 1339 at 150 and 250 mg/kg caused profound hypertriglyceridemia. Triglyceride concentrations increased in a curvilinear fashion for the first 2 hours and remained increased for approximately 24 hours. Area under the time-concentration curve for triglyceride at 5 hours differed significantly among groups. At 12 and 24 hours, cholesterol was significantly higher in cats receiving 250 mg/kg. The most dramatic changes in osmotic fragility and RBC morphology were in cats receiving 250 mg/kg; 1 of these cats developed severe icterus and died 5 days later. Feeding rice and casein before administering Triton WR 1339 at 150 mg/kg did not appear to affect the hypertriglyceridemia response.

Conclusions and Clinical Relevance—Triton WR 1339 can be administered IV to cats at a rate of 150 mg/kg to assess hepatic triglyceride secretion, although some cats may have increased RBC osmotic fragility. Higher dosages caused substantial adverse effects, whereas lower dosages did not alter plasma triglyceride concentration. (Am J Vet Res 2000;61:941–950)