Oxidant stress in sled dogs subjected to repetitive endurance exercise

Kenneth W. Hinchcliff Department of Veterinary Clinical Sciences, College of Veterinary Medicine, The Ohio State University, Columbus, OH 43210.

Search for other papers by Kenneth W. Hinchcliff in
Current site
Google Scholar
PubMed
Close
 BVSc, PhD
,
Gregory A. Reinhart Research and Development, The Iams Company, Lewisburg, OH 45338.

Search for other papers by Gregory A. Reinhart in
Current site
Google Scholar
PubMed
Close
 PhD
,
Robert DiSilvestro Department of Nutrition and Food Management, The Ohio State University, Columbus, OH 43210.

Search for other papers by Robert DiSilvestro in
Current site
Google Scholar
PubMed
Close
 PhD
,
Arleigh Reynolds Department of Clinical Sciences, College of Veterinary Medicine, Cornell University, Ithaca, NY 14853.

Search for other papers by Arleigh Reynolds in
Current site
Google Scholar
PubMed
Close
 DVM, PhD
,
Ashley Blostein-Fujii Department of Nutrition and Food Management, The Ohio State University, Columbus, OH 43210.

Search for other papers by Ashley Blostein-Fujii in
Current site
Google Scholar
PubMed
Close
 PhD
, and
Richard A. Swenson Lightning Bolt Express Kennels, Two Rivers, AK 99716.

Search for other papers by Richard A. Swenson in
Current site
Google Scholar
PubMed
Close

Abstract

Objective—To determine whether repetitive endurance exercise in sled dogs was associated with substantial lipid peroxidation, decreases in antioxidant capacity of the serum, and skeletal muscle damage.

Animals—24 lightly trained sled dogs.

Procedure—16 dogs completed a 58-km run on each of 3 consecutive days; the other 8 dogs (control) did not exercise during the study. Blood samples were collected before the first exercise run and after the first and third exercise runs. Plasma isoprostane and serum vitamin E concentrations, total antioxidant status of plasma, and serum creatine kinase activity were measured.

Results—Plasma isoprostane concentrations in dogs in the exercise group were significantly increased after the first exercise run and further significantly increased after the third exercise run. Serum vitamin E concentration was significantly decreased after the first exercise run in dogs in the exercise group, and this change persisted after the third exercise run. There was a significant linear relationship between plasma isoprostane concentration and the logarithm of serum creatine kinase activity (adjusted r2 = 0.84).

Conclusions and Clinical Relevance—Results demonstrate that repetitive endurance exercise in dogs is associated with lipid peroxidation and a reduction in plasma antioxidant concentrations. We interpret these results as indicating that the antioxidant mechanisms of minimally trained dogs may, in some instances, be inadequate to meet the antioxidant requirements of repetitive endurance exercise. (Am J Vet Res 2000;61:512–517)

Abstract

Objective—To determine whether repetitive endurance exercise in sled dogs was associated with substantial lipid peroxidation, decreases in antioxidant capacity of the serum, and skeletal muscle damage.

Animals—24 lightly trained sled dogs.

Procedure—16 dogs completed a 58-km run on each of 3 consecutive days; the other 8 dogs (control) did not exercise during the study. Blood samples were collected before the first exercise run and after the first and third exercise runs. Plasma isoprostane and serum vitamin E concentrations, total antioxidant status of plasma, and serum creatine kinase activity were measured.

Results—Plasma isoprostane concentrations in dogs in the exercise group were significantly increased after the first exercise run and further significantly increased after the third exercise run. Serum vitamin E concentration was significantly decreased after the first exercise run in dogs in the exercise group, and this change persisted after the third exercise run. There was a significant linear relationship between plasma isoprostane concentration and the logarithm of serum creatine kinase activity (adjusted r2 = 0.84).

Conclusions and Clinical Relevance—Results demonstrate that repetitive endurance exercise in dogs is associated with lipid peroxidation and a reduction in plasma antioxidant concentrations. We interpret these results as indicating that the antioxidant mechanisms of minimally trained dogs may, in some instances, be inadequate to meet the antioxidant requirements of repetitive endurance exercise. (Am J Vet Res 2000;61:512–517)

All Time Past Year Past 30 Days
Abstract Views 92 0 0
Full Text Views 1044 656 259
PDF Downloads 358 139 12
Advertisement