Myeloperoxidase activity of the large intestine in an equine model of acute colitis

Rebecca S. McConnico From the Departments of Food Animal and Equine Medicine (McConnico, Poston, Roberts) and Microbiology, Pathology, and Parasitology (Weinstock), College of Veterinary Medicine, North Carolina State University, Raleigh, NC 27606.

Search for other papers by Rebecca S. McConnico in
Current site
Google Scholar
PubMed
Close
 DVM, PhD
,
Daniel Weinstock From the Departments of Food Animal and Equine Medicine (McConnico, Poston, Roberts) and Microbiology, Pathology, and Parasitology (Weinstock), College of Veterinary Medicine, North Carolina State University, Raleigh, NC 27606.

Search for other papers by Daniel Weinstock in
Current site
Google Scholar
PubMed
Close
 DVM, PhD
,
Mary Elizabeth Poston From the Departments of Food Animal and Equine Medicine (McConnico, Poston, Roberts) and Microbiology, Pathology, and Parasitology (Weinstock), College of Veterinary Medicine, North Carolina State University, Raleigh, NC 27606.

Search for other papers by Mary Elizabeth Poston in
Current site
Google Scholar
PubMed
Close
 MS, MD
, and
Malcolm C. Roberts From the Departments of Food Animal and Equine Medicine (McConnico, Poston, Roberts) and Microbiology, Pathology, and Parasitology (Weinstock), College of Veterinary Medicine, North Carolina State University, Raleigh, NC 27606.

Search for other papers by Malcolm C. Roberts in
Current site
Google Scholar
PubMed
Close
 BVSC, PhD

Click on author name to view affiliation information

Abstract

Objective

To determine whether quantification of myeloperoxidase (MPO) activity could be a useful laboratory technique to detect granulocyte infiltration in equine intestinal tissues.

Sample Population

Intestinal tissue (inflamed or healthy) collected from 16 age- and sex-matched Shetland Ponies.

Procedure

Intestinal tissue MPO activity was determined, and histologic assessment of adjacent specimens from healthy and inflamed intestine was done.

Results

Intestinal tissue MPO activity and histopathologic score increased with time after castor oil challenge and peaked at 16 hours in an equine diarrhea model in which individual ponies provided their own control tissues.

Conclusions

Intestinal tissue inflammation scores correlated positively with tissue MPO activity in adjacent specimens.

Clinical Relevance

Tissue MPO assay may be a useful laboratory tool to quantify intestinal mucosal inflammation in ponies. (Am J Vet Res 1999;60:807–813)

Abstract

Objective

To determine whether quantification of myeloperoxidase (MPO) activity could be a useful laboratory technique to detect granulocyte infiltration in equine intestinal tissues.

Sample Population

Intestinal tissue (inflamed or healthy) collected from 16 age- and sex-matched Shetland Ponies.

Procedure

Intestinal tissue MPO activity was determined, and histologic assessment of adjacent specimens from healthy and inflamed intestine was done.

Results

Intestinal tissue MPO activity and histopathologic score increased with time after castor oil challenge and peaked at 16 hours in an equine diarrhea model in which individual ponies provided their own control tissues.

Conclusions

Intestinal tissue inflammation scores correlated positively with tissue MPO activity in adjacent specimens.

Clinical Relevance

Tissue MPO assay may be a useful laboratory tool to quantify intestinal mucosal inflammation in ponies. (Am J Vet Res 1999;60:807–813)

All Time Past Year Past 30 Days
Abstract Views 0 0 0
Full Text Views 154 154 44
PDF Downloads 31 31 10
Advertisement