Colonic lymphocyte and plasma cell populations in dogs with lymphocytic-plasmacytic colitis

Albert E. Jergens From the Departments of Veterinary Clinical Sciences (Jergens, Gamet) and Veterinary Pathology (Niyo, Smith), College of Veterinary Medicine, and the Department of Statistics, College of Liberal Arts and Sciences (Tsao), Iowa State University, Ames, IA 50011; and Veterinary Division, Marshfield Laboratories, Marshfield, WI 54449-5795 (Moore).

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Yvan Gamet From the Departments of Veterinary Clinical Sciences (Jergens, Gamet) and Veterinary Pathology (Niyo, Smith), College of Veterinary Medicine, and the Department of Statistics, College of Liberal Arts and Sciences (Tsao), Iowa State University, Ames, IA 50011; and Veterinary Division, Marshfield Laboratories, Marshfield, WI 54449-5795 (Moore).

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Frances M. Moore From the Departments of Veterinary Clinical Sciences (Jergens, Gamet) and Veterinary Pathology (Niyo, Smith), College of Veterinary Medicine, and the Department of Statistics, College of Liberal Arts and Sciences (Tsao), Iowa State University, Ames, IA 50011; and Veterinary Division, Marshfield Laboratories, Marshfield, WI 54449-5795 (Moore).

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Yosiya Niyo From the Departments of Veterinary Clinical Sciences (Jergens, Gamet) and Veterinary Pathology (Niyo, Smith), College of Veterinary Medicine, and the Department of Statistics, College of Liberal Arts and Sciences (Tsao), Iowa State University, Ames, IA 50011; and Veterinary Division, Marshfield Laboratories, Marshfield, WI 54449-5795 (Moore).

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Claire Tsao From the Departments of Veterinary Clinical Sciences (Jergens, Gamet) and Veterinary Pathology (Niyo, Smith), College of Veterinary Medicine, and the Department of Statistics, College of Liberal Arts and Sciences (Tsao), Iowa State University, Ames, IA 50011; and Veterinary Division, Marshfield Laboratories, Marshfield, WI 54449-5795 (Moore).

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Becky Smith From the Departments of Veterinary Clinical Sciences (Jergens, Gamet) and Veterinary Pathology (Niyo, Smith), College of Veterinary Medicine, and the Department of Statistics, College of Liberal Arts and Sciences (Tsao), Iowa State University, Ames, IA 50011; and Veterinary Division, Marshfield Laboratories, Marshfield, WI 54449-5795 (Moore).

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Abstract

Objectives

To quantitate immunoglobulin-containing cells (IgA, IgG, and IgM) and CD3+ T cells in colonic biopsy specimens obtained from dogs with lymphocytic-plasmacytic colitis (LPC), and to compare lymphocyte and plasma cell populations in dogs with LPC with those in healthy dogs.

Animals

10 healthy dogs and 11 dogs with LPC.

Procedure

Colonic mucosal specimens obtained from healthy dogs and dogs with LPC were stained specifically for IgA-, IgG-, and IgM-containing cells and CD3+ T cells by use of immunoperoxidase techniques. Morphometric analyses were done to quantitate lymphocytes and plasma cells in standardized areas of colonic mucosa. Data analyses allowed determination of mean cell numbers in each dog group, and comparison of mean numbers of lymphocytes and plasma cells between dog groups.

Results

CD3+ T cells predominated in healthy dogs, whereas CD3+ T cells and IgA-containing cells were most numerous in dogs with LPC. In both dog groups, the IgG- and IgM-containing cells were considerably less numerous than the other 2 cell types. Comparison of cell populations between dog groups indicated that IgA- and IgG-containing cells and CD3+ T cells were significantly more numerous in the colonic mucosa of dogs with LPC.

Conclusions

Dogs with LPC have significantly increased numbers of IgA- and IgG-containing cells and CD3+ T cells. These lymphocyte and plasma cell distributions indicate similarities to and differences from such distributions in human beings with inflammatory bowel disease. Results provide a basis for future correlation between histologic stage of disease activity and immunologic findings in dogs with LPC. (Am J Vet Res 1999;60:515-520)

Abstract

Objectives

To quantitate immunoglobulin-containing cells (IgA, IgG, and IgM) and CD3+ T cells in colonic biopsy specimens obtained from dogs with lymphocytic-plasmacytic colitis (LPC), and to compare lymphocyte and plasma cell populations in dogs with LPC with those in healthy dogs.

Animals

10 healthy dogs and 11 dogs with LPC.

Procedure

Colonic mucosal specimens obtained from healthy dogs and dogs with LPC were stained specifically for IgA-, IgG-, and IgM-containing cells and CD3+ T cells by use of immunoperoxidase techniques. Morphometric analyses were done to quantitate lymphocytes and plasma cells in standardized areas of colonic mucosa. Data analyses allowed determination of mean cell numbers in each dog group, and comparison of mean numbers of lymphocytes and plasma cells between dog groups.

Results

CD3+ T cells predominated in healthy dogs, whereas CD3+ T cells and IgA-containing cells were most numerous in dogs with LPC. In both dog groups, the IgG- and IgM-containing cells were considerably less numerous than the other 2 cell types. Comparison of cell populations between dog groups indicated that IgA- and IgG-containing cells and CD3+ T cells were significantly more numerous in the colonic mucosa of dogs with LPC.

Conclusions

Dogs with LPC have significantly increased numbers of IgA- and IgG-containing cells and CD3+ T cells. These lymphocyte and plasma cell distributions indicate similarities to and differences from such distributions in human beings with inflammatory bowel disease. Results provide a basis for future correlation between histologic stage of disease activity and immunologic findings in dogs with LPC. (Am J Vet Res 1999;60:515-520)

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