In vitro efficacy of N-duopropenide, a recently developed disinfectant containing quaternary ammonium compounds, against selected gram-positive and gram-negative organisms

C. B. Gutiérrez From the Department of Animal Health, Microbiology and Immunology Section, Faculty of Veterinary Medicine, University of León, Spain.

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D. Álvarez From the Department of Animal Health, Microbiology and Immunology Section, Faculty of Veterinary Medicine, University of León, Spain.

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J. I. Rodríguez-Barbosa From the Department of Animal Health, Microbiology and Immunology Section, Faculty of Veterinary Medicine, University of León, Spain.

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R. I. Tascón From the Department of Animal Health, Microbiology and Immunology Section, Faculty of Veterinary Medicine, University of León, Spain.

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V. A. de la Puente From the Department of Animal Health, Microbiology and Immunology Section, Faculty of Veterinary Medicine, University of León, Spain.

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E. F. Rodríguez-Ferri From the Department of Animal Health, Microbiology and Immunology Section, Faculty of Veterinary Medicine, University of León, Spain.

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Abstract

Objective

To study the efficacy of N-duopropenide against various gram-positive and gram-negative organisms.

Sample Population

One field strain each of Pasteurella multocida subsp multocida, Staphylococcus aureus, Listeria monocytogenes, and L ivanovii, and 2 field strains of Escherichia coli.

Procedure

Strains were tested with and without serum as organic matter, using quantitative suspension and carrier tests. Six concentrations of active ingredient (0.005, 0.01, 0.05, 0.11, 0.27, and 0.55%) and 6 contact times (15 and 30 seconds and 1, 3, 5, and 10 minutes) were studied for each.

Results

Globally, N-duopropenide was more effective in suspension tests than in carrier tests, and when organisms were suspended in saline solution rather than serum. Under the most disadvantageous conditions (carrier test with serum), a concentration of 0.55% N-duopropenide acting for only 15 seconds was effective in inactivating P multocida subsp multocida and was even more effective against the 2 Listeria species tested. For E coli strains, the same concentration also was effective, but after 10 minutes of contact. On the other hand, N-duopropenide was unable to inactivate the S aureus strain in the carrier test with serum, a concentration of 0.55% for 10 minutes was necessary to inactivate it without organic matter; however, N-duopropenide was highly effective against this organism in the suspension test, even with serum.

Conclusion

N-duopropenide was highly effective in vitro against 5 of the most commonly encountered organisms in clinical veterinary medicine and, consequently, might be a good choice in control measures against common pathogenic organisms in modern production systems. (Am J Vet Res 999:60:481-484)

Abstract

Objective

To study the efficacy of N-duopropenide against various gram-positive and gram-negative organisms.

Sample Population

One field strain each of Pasteurella multocida subsp multocida, Staphylococcus aureus, Listeria monocytogenes, and L ivanovii, and 2 field strains of Escherichia coli.

Procedure

Strains were tested with and without serum as organic matter, using quantitative suspension and carrier tests. Six concentrations of active ingredient (0.005, 0.01, 0.05, 0.11, 0.27, and 0.55%) and 6 contact times (15 and 30 seconds and 1, 3, 5, and 10 minutes) were studied for each.

Results

Globally, N-duopropenide was more effective in suspension tests than in carrier tests, and when organisms were suspended in saline solution rather than serum. Under the most disadvantageous conditions (carrier test with serum), a concentration of 0.55% N-duopropenide acting for only 15 seconds was effective in inactivating P multocida subsp multocida and was even more effective against the 2 Listeria species tested. For E coli strains, the same concentration also was effective, but after 10 minutes of contact. On the other hand, N-duopropenide was unable to inactivate the S aureus strain in the carrier test with serum, a concentration of 0.55% for 10 minutes was necessary to inactivate it without organic matter; however, N-duopropenide was highly effective against this organism in the suspension test, even with serum.

Conclusion

N-duopropenide was highly effective in vitro against 5 of the most commonly encountered organisms in clinical veterinary medicine and, consequently, might be a good choice in control measures against common pathogenic organisms in modern production systems. (Am J Vet Res 999:60:481-484)

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