Identification of serotype by use of serologic assay and detection of the enterotoxin gene of Escherichia coli by means of a polymerase chain reaction assay for isolates from pigs, chickens, and cows

Hee Kon Jung From the Department of Food and Nutrition, Songwon College, 199-1, Kwangchcong-dong, Seo-gu, Kwang-ju, Republic of Korea (South Korea), Korea 502-742.

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 DVM, MPH

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Abstract

Objective

To serotype an enterotoxin gene from Escherichia coli isolated from cows, pigs, and chickens in Korea.

Sample population

Isolates from 37 cows with mastitis, 51 diarrheic pigs, and 5 diarrheic chickens.

Procedure

Serogroups and serotypes were identified by slide agglutination testing, using pathogenic E coli sera. Detection of E coli enterotoxins by use of reversed passive latex agglutination and ELISA was compared by proving existence of the gene by polymerase chain reaction (PCR) analysis.

Results and Conclusions

Detection of E coli enterotoxin by either method was positive for 1 strain (O20:H10; heat-labile enterotoxin [LP+], heat-stable enterotoxin [STa+]; isolation rate, 2%) and 3 other strains (0111:H10, 0119:H9, and 0125:H6, STa+; isolation rate, 5.9%) isolated from fecal specimens obtained from diarrheic pigs. The E coli enterotoxin genes were identified by use of PCR analysis in 1 strain containing the 417- and 163-base pair (bp) genes (LT+, Sta+; O20:H10) and in 3 strains containing only the 163-bp gene (STa+; 0111:H10, 0119:H9, and 0125:H6).

Clinical Relevance

Serotyping of E coli enterotoxin may be used to analyze patterns of transmission among species of domestic animals. (Am J Vet Res 1999;60:468-472)

Abstract

Objective

To serotype an enterotoxin gene from Escherichia coli isolated from cows, pigs, and chickens in Korea.

Sample population

Isolates from 37 cows with mastitis, 51 diarrheic pigs, and 5 diarrheic chickens.

Procedure

Serogroups and serotypes were identified by slide agglutination testing, using pathogenic E coli sera. Detection of E coli enterotoxins by use of reversed passive latex agglutination and ELISA was compared by proving existence of the gene by polymerase chain reaction (PCR) analysis.

Results and Conclusions

Detection of E coli enterotoxin by either method was positive for 1 strain (O20:H10; heat-labile enterotoxin [LP+], heat-stable enterotoxin [STa+]; isolation rate, 2%) and 3 other strains (0111:H10, 0119:H9, and 0125:H6, STa+; isolation rate, 5.9%) isolated from fecal specimens obtained from diarrheic pigs. The E coli enterotoxin genes were identified by use of PCR analysis in 1 strain containing the 417- and 163-base pair (bp) genes (LT+, Sta+; O20:H10) and in 3 strains containing only the 163-bp gene (STa+; 0111:H10, 0119:H9, and 0125:H6).

Clinical Relevance

Serotyping of E coli enterotoxin may be used to analyze patterns of transmission among species of domestic animals. (Am J Vet Res 1999;60:468-472)

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