Lipid peroxides and antioxidants in serum of neonatal calves

Osamu Inanami From the Department of Environmental Veterinary Sciences, Graduate School of Veterinary Medicine, Hokkaido University, Sapporo 060, Japan (Inanami, Kuwabara), and the Department of Animal Nutrition and Feeding (Shiga), Veterinary Hospital (Okada), Department of Veterinary Internal Medicine (Sato), and the Laboratory of Theriogenology (Miyake), Faculty of Agriculture, Iwate University, Iwate 020, Japan.

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Akio Shiga From the Department of Environmental Veterinary Sciences, Graduate School of Veterinary Medicine, Hokkaido University, Sapporo 060, Japan (Inanami, Kuwabara), and the Department of Animal Nutrition and Feeding (Shiga), Veterinary Hospital (Okada), Department of Veterinary Internal Medicine (Sato), and the Laboratory of Theriogenology (Miyake), Faculty of Agriculture, Iwate University, Iwate 020, Japan.

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Keiji Okada From the Department of Environmental Veterinary Sciences, Graduate School of Veterinary Medicine, Hokkaido University, Sapporo 060, Japan (Inanami, Kuwabara), and the Department of Animal Nutrition and Feeding (Shiga), Veterinary Hospital (Okada), Department of Veterinary Internal Medicine (Sato), and the Laboratory of Theriogenology (Miyake), Faculty of Agriculture, Iwate University, Iwate 020, Japan.

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Reeko Sato From the Department of Environmental Veterinary Sciences, Graduate School of Veterinary Medicine, Hokkaido University, Sapporo 060, Japan (Inanami, Kuwabara), and the Department of Animal Nutrition and Feeding (Shiga), Veterinary Hospital (Okada), Department of Veterinary Internal Medicine (Sato), and the Laboratory of Theriogenology (Miyake), Faculty of Agriculture, Iwate University, Iwate 020, Japan.

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Yoh-ich Miyake From the Department of Environmental Veterinary Sciences, Graduate School of Veterinary Medicine, Hokkaido University, Sapporo 060, Japan (Inanami, Kuwabara), and the Department of Animal Nutrition and Feeding (Shiga), Veterinary Hospital (Okada), Department of Veterinary Internal Medicine (Sato), and the Laboratory of Theriogenology (Miyake), Faculty of Agriculture, Iwate University, Iwate 020, Japan.

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Mikinori Kuwabara From the Department of Environmental Veterinary Sciences, Graduate School of Veterinary Medicine, Hokkaido University, Sapporo 060, Japan (Inanami, Kuwabara), and the Department of Animal Nutrition and Feeding (Shiga), Veterinary Hospital (Okada), Department of Veterinary Internal Medicine (Sato), and the Laboratory of Theriogenology (Miyake), Faculty of Agriculture, Iwate University, Iwate 020, Japan.

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Abstract

Objective

To examine the association between oxidative stress and antioxidants in neonatal calves after birth.

Sample Population

Sera from 6 healthy Holstein-Friesian cows and 7 of their newborn calves were obtained at various intervals after birth.

Procedure

Lipid peroxides in serum of cows and their newborn calves were estimated by measuring concentrations of thiobarbituric acid-reactive substances (TBARS). The antioxidative activities of neonatal sera were evaluated by measuring their superoxide-scavenging activities, ferroxidase activities, and the concentration of bilirubin-associated albumin.

Results

Concentration of TBARS in neonatal sera within 1 day after birth was significantly higher than concentrations ≥ 2 days after birth and concentrations in serum of the dams. In contrast, antioxidative activities of neonatal sera, evaluated on the basis of superoxide-scavenging activities, ferroxidase activities, and concentration of bilirubin-associated albumin 3 hours after birth, were significantly lower than antioxidative activities in sera of the dams.

Conclusions

Susceptibility of calves to oxidative stress during the neonatal period may be explained by the immature defense system against superoxide radicals. (Am J Vet Res 1999;60:452-457)

Abstract

Objective

To examine the association between oxidative stress and antioxidants in neonatal calves after birth.

Sample Population

Sera from 6 healthy Holstein-Friesian cows and 7 of their newborn calves were obtained at various intervals after birth.

Procedure

Lipid peroxides in serum of cows and their newborn calves were estimated by measuring concentrations of thiobarbituric acid-reactive substances (TBARS). The antioxidative activities of neonatal sera were evaluated by measuring their superoxide-scavenging activities, ferroxidase activities, and the concentration of bilirubin-associated albumin.

Results

Concentration of TBARS in neonatal sera within 1 day after birth was significantly higher than concentrations ≥ 2 days after birth and concentrations in serum of the dams. In contrast, antioxidative activities of neonatal sera, evaluated on the basis of superoxide-scavenging activities, ferroxidase activities, and concentration of bilirubin-associated albumin 3 hours after birth, were significantly lower than antioxidative activities in sera of the dams.

Conclusions

Susceptibility of calves to oxidative stress during the neonatal period may be explained by the immature defense system against superoxide radicals. (Am J Vet Res 1999;60:452-457)

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