Measurement of synovial fluid and serum concentrations of the 846 epitope of chondroitin sulfate and of carboxy propeptides of type II procollagen for diagnosis of osteochondral fragmentation in horses

David D. Frisbie From the Equine Orthopaedic Research Laboratory, College of Veterinary Medicine, Colorado State University, Fort Collins, CO, 80524 (Frisbie, Ray, Chapman, McIlwraith); and the Joint Diseases Laboratory, Shriners Hospitals for Children; and Division of Surgical Research, Department of Surgery, McGill University, Montreal, Quebec, Canada H361A6 (Ionescu, Poole).

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 DVM, MS
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Christopher S. Ray From the Equine Orthopaedic Research Laboratory, College of Veterinary Medicine, Colorado State University, Fort Collins, CO, 80524 (Frisbie, Ray, Chapman, McIlwraith); and the Joint Diseases Laboratory, Shriners Hospitals for Children; and Division of Surgical Research, Department of Surgery, McGill University, Montreal, Quebec, Canada H361A6 (Ionescu, Poole).

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Mirela Ionescu From the Equine Orthopaedic Research Laboratory, College of Veterinary Medicine, Colorado State University, Fort Collins, CO, 80524 (Frisbie, Ray, Chapman, McIlwraith); and the Joint Diseases Laboratory, Shriners Hospitals for Children; and Division of Surgical Research, Department of Surgery, McGill University, Montreal, Quebec, Canada H361A6 (Ionescu, Poole).

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A. Robin Poole From the Equine Orthopaedic Research Laboratory, College of Veterinary Medicine, Colorado State University, Fort Collins, CO, 80524 (Frisbie, Ray, Chapman, McIlwraith); and the Joint Diseases Laboratory, Shriners Hospitals for Children; and Division of Surgical Research, Department of Surgery, McGill University, Montreal, Quebec, Canada H361A6 (Ionescu, Poole).

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Phillip L. Chapman From the Equine Orthopaedic Research Laboratory, College of Veterinary Medicine, Colorado State University, Fort Collins, CO, 80524 (Frisbie, Ray, Chapman, McIlwraith); and the Joint Diseases Laboratory, Shriners Hospitals for Children; and Division of Surgical Research, Department of Surgery, McGill University, Montreal, Quebec, Canada H361A6 (Ionescu, Poole).

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C. Wayne McIlwraith From the Equine Orthopaedic Research Laboratory, College of Veterinary Medicine, Colorado State University, Fort Collins, CO, 80524 (Frisbie, Ray, Chapman, McIlwraith); and the Joint Diseases Laboratory, Shriners Hospitals for Children; and Division of Surgical Research, Department of Surgery, McGill University, Montreal, Quebec, Canada H361A6 (Ionescu, Poole).

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 BVSc, PhD

Abstract

Objective

To determine whether serum or synovial fluid concentrations of chondroitin sulfate epitope 846 and carboxy propeptides of type II collagen (CPII) can be used to diagnose osteochondral fragmentation (OC) in horses.

Animals

38 horses with unilateral OC of the radiocarpal (n = 31) or intercarpal (33) joints and 8 clinically and radiographically normal horses.

Procedures

For horses with OC, serum and synovial fluid concentrations of epitope 846, CPII, and keratan sulfate (KS) were determined, along with synovial fluid WBC counts and total protein concentrations. Serum epitope 846, CPII, and KS concentrations were measured in control horses.

Results

Synovial fluid epitope 846 and total protein concentrations were significantly higher in the joints with OC than in unaffected joints, but CPII and KS concentrations and WBC counts were not. Synovial fluid total protein and 846 epitope concentrations were linearly related to grade of OC. Serum epitope 846 and CPII concentrations were significantly higher in horses with OC than in control horses. Discriminant analysis allowed 27 of 34 (79%) horses to be correctly classified as having or not having OC on the basis of serum epitope 846 and CPII concentrations.

Conclusions

Results suggest that serum and synovial fluid concentrations of epitope 846 and CPII are associated with OC. Increases in concentrations of epitope 846 and CPII suggest that increased synthesis of cartilage aggrecan and type II procollagen may be associated with OC.

Clinical Relevance

Measurement of serum epitope 846 and CPII concentrations may be useful in the diagnosis of OC in horses. (Am J Vet Res 1999;60:306–309)

Abstract

Objective

To determine whether serum or synovial fluid concentrations of chondroitin sulfate epitope 846 and carboxy propeptides of type II collagen (CPII) can be used to diagnose osteochondral fragmentation (OC) in horses.

Animals

38 horses with unilateral OC of the radiocarpal (n = 31) or intercarpal (33) joints and 8 clinically and radiographically normal horses.

Procedures

For horses with OC, serum and synovial fluid concentrations of epitope 846, CPII, and keratan sulfate (KS) were determined, along with synovial fluid WBC counts and total protein concentrations. Serum epitope 846, CPII, and KS concentrations were measured in control horses.

Results

Synovial fluid epitope 846 and total protein concentrations were significantly higher in the joints with OC than in unaffected joints, but CPII and KS concentrations and WBC counts were not. Synovial fluid total protein and 846 epitope concentrations were linearly related to grade of OC. Serum epitope 846 and CPII concentrations were significantly higher in horses with OC than in control horses. Discriminant analysis allowed 27 of 34 (79%) horses to be correctly classified as having or not having OC on the basis of serum epitope 846 and CPII concentrations.

Conclusions

Results suggest that serum and synovial fluid concentrations of epitope 846 and CPII are associated with OC. Increases in concentrations of epitope 846 and CPII suggest that increased synthesis of cartilage aggrecan and type II procollagen may be associated with OC.

Clinical Relevance

Measurement of serum epitope 846 and CPII concentrations may be useful in the diagnosis of OC in horses. (Am J Vet Res 1999;60:306–309)

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