Detection of annexin I and IV and haptoglobin in bronchoalveolar lavage fluid from calves experimentally inoculated with Pasteurella haemolytica

Norio Katoh From the Department of Systematic Diagnosis, National Institute of Animal Health, 3-1-1 Kannondai, Tsukuba, lbaraki 305-0856 (Katoh, Miyamoto, Watanabe); and the Ishikawa Nanbu Livestock Hygiene Service Center, Kanazawa, Ishikawa 920-3101, Japan (Nakagawa).

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Toru Miyamoto From the Department of Systematic Diagnosis, National Institute of Animal Health, 3-1-1 Kannondai, Tsukuba, lbaraki 305-0856 (Katoh, Miyamoto, Watanabe); and the Ishikawa Nanbu Livestock Hygiene Service Center, Kanazawa, Ishikawa 920-3101, Japan (Nakagawa).

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Hisami Nakagawa From the Department of Systematic Diagnosis, National Institute of Animal Health, 3-1-1 Kannondai, Tsukuba, lbaraki 305-0856 (Katoh, Miyamoto, Watanabe); and the Ishikawa Nanbu Livestock Hygiene Service Center, Kanazawa, Ishikawa 920-3101, Japan (Nakagawa).

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Atsushi Watanabe From the Department of Systematic Diagnosis, National Institute of Animal Health, 3-1-1 Kannondai, Tsukuba, lbaraki 305-0856 (Katoh, Miyamoto, Watanabe); and the Ishikawa Nanbu Livestock Hygiene Service Center, Kanazawa, Ishikawa 920-3101, Japan (Nakagawa).

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Abstract

Objective

To determine whether annexins or haptoglobin could be detected in bronchoalveolar lavage (BAL) fluid specimens obtained from calves experimentally inoculated with Pasteurella haemolytica.

Animals

Twelve 2- to 3-month-old male Holstein calves.

Procedure

Pasteurella haemolytica was inoculated into the right lung lobes of each of 6 calves. Six other calves received vehicle alone and were used as control calves. Specimens of BAL fluid were obtained from 3 control and 3 inoculated calves 1 day after inoculation and from the other calves 2 days after inoculation. The amount of annexins I, II, IV, and VI, and haptoglobin in BAL fluid specimens was examined by use of immunoblot analysis.

Results

Annexins I and IV were detected in BAL fluid specimens obtained from the right lung lobes of each of the inoculated calves, but annexins II and VI were not. Annexin I also was found in BAL fluid specimens obtained from the left lung lobes of each inoculated calf and from left and right lung lobes of the control calves. By comparison, detection of annexin IV was essentially limited to the right lung lobes of inoculated calves. Haptoglobin was detected in some, but not all, BAL fluid specimens from the right lung lobes of inoculated calves, and its detection in BAL fluid was associated with serum proteins such as albumin.

Conclusions and Clinical Relevance

Annexin IV was detected most specifically in response to inoculation of P haemolytica. This protein could be used as a marker for inflammatory pulmonary disease caused by P haemolytica. (Am J Vet Res 1999;60:1390–1395)

Abstract

Objective

To determine whether annexins or haptoglobin could be detected in bronchoalveolar lavage (BAL) fluid specimens obtained from calves experimentally inoculated with Pasteurella haemolytica.

Animals

Twelve 2- to 3-month-old male Holstein calves.

Procedure

Pasteurella haemolytica was inoculated into the right lung lobes of each of 6 calves. Six other calves received vehicle alone and were used as control calves. Specimens of BAL fluid were obtained from 3 control and 3 inoculated calves 1 day after inoculation and from the other calves 2 days after inoculation. The amount of annexins I, II, IV, and VI, and haptoglobin in BAL fluid specimens was examined by use of immunoblot analysis.

Results

Annexins I and IV were detected in BAL fluid specimens obtained from the right lung lobes of each of the inoculated calves, but annexins II and VI were not. Annexin I also was found in BAL fluid specimens obtained from the left lung lobes of each inoculated calf and from left and right lung lobes of the control calves. By comparison, detection of annexin IV was essentially limited to the right lung lobes of inoculated calves. Haptoglobin was detected in some, but not all, BAL fluid specimens from the right lung lobes of inoculated calves, and its detection in BAL fluid was associated with serum proteins such as albumin.

Conclusions and Clinical Relevance

Annexin IV was detected most specifically in response to inoculation of P haemolytica. This protein could be used as a marker for inflammatory pulmonary disease caused by P haemolytica. (Am J Vet Res 1999;60:1390–1395)

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