Ranitidine bismuth citrate and clarithromycin, alone or in combination, for eradication of Helicobacter mustelae infection in ferrets

Robert P. Marini From the Division of Comparative Medicine, Massachusetts Institute of Technology, Cambridge, MA 02139 (Marini, Fox, Taylor, Yan); and Glaxo Wellcome, Stevenage, UK (McColm, Williamson).

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James G. Fox From the Division of Comparative Medicine, Massachusetts Institute of Technology, Cambridge, MA 02139 (Marini, Fox, Taylor, Yan); and Glaxo Wellcome, Stevenage, UK (McColm, Williamson).

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Nancy S. Taylor From the Division of Comparative Medicine, Massachusetts Institute of Technology, Cambridge, MA 02139 (Marini, Fox, Taylor, Yan); and Glaxo Wellcome, Stevenage, UK (McColm, Williamson).

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Lili Yan From the Division of Comparative Medicine, Massachusetts Institute of Technology, Cambridge, MA 02139 (Marini, Fox, Taylor, Yan); and Glaxo Wellcome, Stevenage, UK (McColm, Williamson).

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Andrew A. McColm From the Division of Comparative Medicine, Massachusetts Institute of Technology, Cambridge, MA 02139 (Marini, Fox, Taylor, Yan); and Glaxo Wellcome, Stevenage, UK (McColm, Williamson).

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Russell Williamson From the Division of Comparative Medicine, Massachusetts Institute of Technology, Cambridge, MA 02139 (Marini, Fox, Taylor, Yan); and Glaxo Wellcome, Stevenage, UK (McColm, Williamson).

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Abstract

Objective

To determine whether ranitidine bismuth citrate, clarithromycin, or a combination of ranitidine bismuth citrate and clarithromycin would be efficacious in eradication of Helicobacter mustelae infection in ferrets.

Animals

60 seven-month-old ferrets.

Procedure

To determine dosages of clarithromycin and ranitidine bismuth citrate that would suppress growth of, but not eradicate infection with, H mustelae, ferrets (n = 6/group) were treated PO with clarithromycin or ranitidine bismuth citrate at various dosages. Efficacy of treatment was then determined by treating ferrets with clarithromycin alone, ranitidine bismuth citrate alone, or clarithromycin and ranitidine bismuth citrate. Gastric biopsy specimens were obtained before, during, and at various times after treatment and submitted for quantitative bacterial culture and histologic evaluation. Minimum concentrations of clarithromycin that inhibited 90% of the growth of isolates obtained before and after treatment were determined.

Results

Dosages of clarithromycin and ranitidine bismuth citrate that suppressed growth of H mustelae were 12.5 and 24 mg/kg of body weight, PO, every 8 hours, respectively. Infection was not eradicated in ferrets treated with ranitidine bismuth citrate alone but was eradicated in all 6 ferrets treated with clarithromycin and ranitidine bismuth citrate and in 4 of 6 treated with clarithromycin alone. A decrease in susceptibility to clarithromycin was detected for H mustelae isolates obtained after treatment. Mild or moderate antral gastritis was observed even in ferrets from which infection was eradicated.

Conclusions and Clinical Relevance

A combination of ranitidine bismuth citrate and clarithromycin was efficacious in eradicating H mustelae infection from ferrets. (Am J Vet Res 1999;60:1280–1286)

Abstract

Objective

To determine whether ranitidine bismuth citrate, clarithromycin, or a combination of ranitidine bismuth citrate and clarithromycin would be efficacious in eradication of Helicobacter mustelae infection in ferrets.

Animals

60 seven-month-old ferrets.

Procedure

To determine dosages of clarithromycin and ranitidine bismuth citrate that would suppress growth of, but not eradicate infection with, H mustelae, ferrets (n = 6/group) were treated PO with clarithromycin or ranitidine bismuth citrate at various dosages. Efficacy of treatment was then determined by treating ferrets with clarithromycin alone, ranitidine bismuth citrate alone, or clarithromycin and ranitidine bismuth citrate. Gastric biopsy specimens were obtained before, during, and at various times after treatment and submitted for quantitative bacterial culture and histologic evaluation. Minimum concentrations of clarithromycin that inhibited 90% of the growth of isolates obtained before and after treatment were determined.

Results

Dosages of clarithromycin and ranitidine bismuth citrate that suppressed growth of H mustelae were 12.5 and 24 mg/kg of body weight, PO, every 8 hours, respectively. Infection was not eradicated in ferrets treated with ranitidine bismuth citrate alone but was eradicated in all 6 ferrets treated with clarithromycin and ranitidine bismuth citrate and in 4 of 6 treated with clarithromycin alone. A decrease in susceptibility to clarithromycin was detected for H mustelae isolates obtained after treatment. Mild or moderate antral gastritis was observed even in ferrets from which infection was eradicated.

Conclusions and Clinical Relevance

A combination of ranitidine bismuth citrate and clarithromycin was efficacious in eradicating H mustelae infection from ferrets. (Am J Vet Res 1999;60:1280–1286)

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