Humoral and cell-mediated immune responses of beef and dairy cattle experimentally infested with Psoroptes ovis

Jean-François Lonneux From the Departments of Parasitology and Pathology of Parasitic Diseases (Lonneux, Nguyen, Losson), Immunology and Vaccinology (Denis, Pastoret), and Virology and Pathology of Viral Diseases (Thiry), Faculty of Veterinary Medicine, B-43 University of Liège, Liège, Belgium; and Schering Plough Animal Health, Rue de Stalle, 67, B-1180, Brussels, Belgium (Hollanders).

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Thu Queen Nguyen From the Departments of Parasitology and Pathology of Parasitic Diseases (Lonneux, Nguyen, Losson), Immunology and Vaccinology (Denis, Pastoret), and Virology and Pathology of Viral Diseases (Thiry), Faculty of Veterinary Medicine, B-43 University of Liège, Liège, Belgium; and Schering Plough Animal Health, Rue de Stalle, 67, B-1180, Brussels, Belgium (Hollanders).

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Wim Hollanders From the Departments of Parasitology and Pathology of Parasitic Diseases (Lonneux, Nguyen, Losson), Immunology and Vaccinology (Denis, Pastoret), and Virology and Pathology of Viral Diseases (Thiry), Faculty of Veterinary Medicine, B-43 University of Liège, Liège, Belgium; and Schering Plough Animal Health, Rue de Stalle, 67, B-1180, Brussels, Belgium (Hollanders).

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Martine Denis From the Departments of Parasitology and Pathology of Parasitic Diseases (Lonneux, Nguyen, Losson), Immunology and Vaccinology (Denis, Pastoret), and Virology and Pathology of Viral Diseases (Thiry), Faculty of Veterinary Medicine, B-43 University of Liège, Liège, Belgium; and Schering Plough Animal Health, Rue de Stalle, 67, B-1180, Brussels, Belgium (Hollanders).

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Etienne Thiry From the Departments of Parasitology and Pathology of Parasitic Diseases (Lonneux, Nguyen, Losson), Immunology and Vaccinology (Denis, Pastoret), and Virology and Pathology of Viral Diseases (Thiry), Faculty of Veterinary Medicine, B-43 University of Liège, Liège, Belgium; and Schering Plough Animal Health, Rue de Stalle, 67, B-1180, Brussels, Belgium (Hollanders).

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Pierre Paul Pastoret From the Departments of Parasitology and Pathology of Parasitic Diseases (Lonneux, Nguyen, Losson), Immunology and Vaccinology (Denis, Pastoret), and Virology and Pathology of Viral Diseases (Thiry), Faculty of Veterinary Medicine, B-43 University of Liège, Liège, Belgium; and Schering Plough Animal Health, Rue de Stalle, 67, B-1180, Brussels, Belgium (Hollanders).

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Bertrand Losson From the Departments of Parasitology and Pathology of Parasitic Diseases (Lonneux, Nguyen, Losson), Immunology and Vaccinology (Denis, Pastoret), and Virology and Pathology of Viral Diseases (Thiry), Faculty of Veterinary Medicine, B-43 University of Liège, Liège, Belgium; and Schering Plough Animal Health, Rue de Stalle, 67, B-1180, Brussels, Belgium (Hollanders).

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Abstract

Objective

To compare cellular and humoral immune responses of beef (Belgian White and Blue [BWB]) and dairy (Friesian-Holstein [FH]) cattle to Psoroptes ovis infestation and to determine whether P ovis infestation impaired immune responses to infectious bovine rhinotracheitis virus (IBR) vaccine or an immunogenic protein (keyhole-limpet hemocyanin [KLH]).

Animals

19 BWB and 6 FH 1-year-old calves.

Procedure

2 trials were performed. In each trial, 7 (trial 1) or 6 (trial 2) BWB calves and 3 FH calves were experimentally infested with P ovis and 3 BWB calves were maintained as uninfested controls. Animals were inoculated with KLH and IBR virus vaccine twice; 3 BWB calves in each trial were treated with ivermectin. Serum antibody responses to KLH, IBR virus, and P ovis were measured by use of ELISA. A lymphocyte transformation assay was used to determine nonspecific responses to 3 mitogens and specific lymphocyte reactivity to P ovis antigen.

Results

In each trial, 3 BWB and 3 FH calves developed clinical signs of psoroptic mange and mites could be recovered. Infested and control animals developed similar antibody titers to KLH and IBR virus. Antibodies to P ovis were detected early in some infested calves, and this was correlated with a marked cell-mediated immune response. Lymphocyte responsiveness to the 3 mitogens was not significantly different among groups.

Conclusions

In these calves, infestation with P ovis induced a marked humoral and cell-mediated immune response. Immunosuppression was not evident. (Am J Vet Res 1998;59:583–587)

Abstract

Objective

To compare cellular and humoral immune responses of beef (Belgian White and Blue [BWB]) and dairy (Friesian-Holstein [FH]) cattle to Psoroptes ovis infestation and to determine whether P ovis infestation impaired immune responses to infectious bovine rhinotracheitis virus (IBR) vaccine or an immunogenic protein (keyhole-limpet hemocyanin [KLH]).

Animals

19 BWB and 6 FH 1-year-old calves.

Procedure

2 trials were performed. In each trial, 7 (trial 1) or 6 (trial 2) BWB calves and 3 FH calves were experimentally infested with P ovis and 3 BWB calves were maintained as uninfested controls. Animals were inoculated with KLH and IBR virus vaccine twice; 3 BWB calves in each trial were treated with ivermectin. Serum antibody responses to KLH, IBR virus, and P ovis were measured by use of ELISA. A lymphocyte transformation assay was used to determine nonspecific responses to 3 mitogens and specific lymphocyte reactivity to P ovis antigen.

Results

In each trial, 3 BWB and 3 FH calves developed clinical signs of psoroptic mange and mites could be recovered. Infested and control animals developed similar antibody titers to KLH and IBR virus. Antibodies to P ovis were detected early in some infested calves, and this was correlated with a marked cell-mediated immune response. Lymphocyte responsiveness to the 3 mitogens was not significantly different among groups.

Conclusions

In these calves, infestation with P ovis induced a marked humoral and cell-mediated immune response. Immunosuppression was not evident. (Am J Vet Res 1998;59:583–587)

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