Water content and glycosaminoglycan disaccharide concentration of the canine meniscus

John S. Stephan From the Department of Clinical Sciences, College of Veterinary Medicine, Kansas State University, Manhattan, KS 66506.

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 DVM, MS
,
Ron M. McLaughlin Jr. From the Department of Clinical Sciences, College of Veterinary Medicine, Kansas State University, Manhattan, KS 66506.

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 DVM, DVSc
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Gary Griffith From the Department of Clinical Sciences, College of Veterinary Medicine, Kansas State University, Manhattan, KS 66506.

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 PhD

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SUMMARY

Objective

To determine the regional composition of water and glycosaminoglycan (GAG) disaccharides of the canine meniscus.

Sample Population

52 menisci from the stifle of dogs.

Procedure

Regional sections of each meniscus were weighed, dried, and reweighed to determine water content. Dried tissue specimens were subjected to enzymatic digestion. Analysis and quantification of disaccharide degradation products were performed, using high-performance liquid chromatography.

Results

Water content was approximately 65% in polar and central regions of the canine meniscus. Water content of the central region of the lateral meniscus was significantly higher than that of the medial meniscus (P = 0.0090). Chondroitinase digestion of canine meniscal tissue yielded detectable ΔDi-HA, ΔDi-4S, and ΔDi-6S GAG disaccharides. Disaccharides specific to dermatan sulfate and chondroitin D or E sulfate were not detected. Concentrations of ΔDi-4S and ΔDi-6S were significantly greater in the lateral central region, compared with the medial central region (P = 0.0005 and 0.0002, respectively).

Conclusion

Water content and ΔDi-4S and ΔDi-6S concentrations were significantly lower in the central region of the medial meniscus, compared with the central region of the lateral meniscus. Reduced tissue hydration of the medial central region may have been a direct result of its overall decrease in total GAG content.

Clinical Relevance

The ability to evaluate subtle differences in tissue GAG composition by analytical measurement of their constituent disaccharides may aid in the understanding of the complex material properties of the normal and diseased meniscus, which may be applied to the study of meniscal healing and biomechanics. (Am J Vet Res 1998;59:213–216)

SUMMARY

Objective

To determine the regional composition of water and glycosaminoglycan (GAG) disaccharides of the canine meniscus.

Sample Population

52 menisci from the stifle of dogs.

Procedure

Regional sections of each meniscus were weighed, dried, and reweighed to determine water content. Dried tissue specimens were subjected to enzymatic digestion. Analysis and quantification of disaccharide degradation products were performed, using high-performance liquid chromatography.

Results

Water content was approximately 65% in polar and central regions of the canine meniscus. Water content of the central region of the lateral meniscus was significantly higher than that of the medial meniscus (P = 0.0090). Chondroitinase digestion of canine meniscal tissue yielded detectable ΔDi-HA, ΔDi-4S, and ΔDi-6S GAG disaccharides. Disaccharides specific to dermatan sulfate and chondroitin D or E sulfate were not detected. Concentrations of ΔDi-4S and ΔDi-6S were significantly greater in the lateral central region, compared with the medial central region (P = 0.0005 and 0.0002, respectively).

Conclusion

Water content and ΔDi-4S and ΔDi-6S concentrations were significantly lower in the central region of the medial meniscus, compared with the central region of the lateral meniscus. Reduced tissue hydration of the medial central region may have been a direct result of its overall decrease in total GAG content.

Clinical Relevance

The ability to evaluate subtle differences in tissue GAG composition by analytical measurement of their constituent disaccharides may aid in the understanding of the complex material properties of the normal and diseased meniscus, which may be applied to the study of meniscal healing and biomechanics. (Am J Vet Res 1998;59:213–216)

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