Correlation between longitudinal bone growth, growth hormone, and insulin-like growth factor-I in prepubertal dogs

Michael G. Conzemius From the Departments of Clinical Studies (Conzemius) and Statistics (Brabec), College of Veterinary Medicine, Iowa State University, Ames, IA 50014; the Department of Clinical Studies, Veterinary Hospital at the University of Pennsylvania, Philadelphia, PA 19104-6010 (Brown, Smith, Washabau, LaFond); and the Research Division, Department of Obstetrics and Gynecology, Uniformed Services University of the Health Sciences, Bethesda, MD 20814-4799 (Chakraborty).

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Dorothy Cimino Brown From the Departments of Clinical Studies (Conzemius) and Statistics (Brabec), College of Veterinary Medicine, Iowa State University, Ames, IA 50014; the Department of Clinical Studies, Veterinary Hospital at the University of Pennsylvania, Philadelphia, PA 19104-6010 (Brown, Smith, Washabau, LaFond); and the Research Division, Department of Obstetrics and Gynecology, Uniformed Services University of the Health Sciences, Bethesda, MD 20814-4799 (Chakraborty).

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Marek Brabec From the Departments of Clinical Studies (Conzemius) and Statistics (Brabec), College of Veterinary Medicine, Iowa State University, Ames, IA 50014; the Department of Clinical Studies, Veterinary Hospital at the University of Pennsylvania, Philadelphia, PA 19104-6010 (Brown, Smith, Washabau, LaFond); and the Research Division, Department of Obstetrics and Gynecology, Uniformed Services University of the Health Sciences, Bethesda, MD 20814-4799 (Chakraborty).

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Gail K. Smith From the Departments of Clinical Studies (Conzemius) and Statistics (Brabec), College of Veterinary Medicine, Iowa State University, Ames, IA 50014; the Department of Clinical Studies, Veterinary Hospital at the University of Pennsylvania, Philadelphia, PA 19104-6010 (Brown, Smith, Washabau, LaFond); and the Research Division, Department of Obstetrics and Gynecology, Uniformed Services University of the Health Sciences, Bethesda, MD 20814-4799 (Chakraborty).

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Robert Washabau From the Departments of Clinical Studies (Conzemius) and Statistics (Brabec), College of Veterinary Medicine, Iowa State University, Ames, IA 50014; the Department of Clinical Studies, Veterinary Hospital at the University of Pennsylvania, Philadelphia, PA 19104-6010 (Brown, Smith, Washabau, LaFond); and the Research Division, Department of Obstetrics and Gynecology, Uniformed Services University of the Health Sciences, Bethesda, MD 20814-4799 (Chakraborty).

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Elizabeth LaFond From the Departments of Clinical Studies (Conzemius) and Statistics (Brabec), College of Veterinary Medicine, Iowa State University, Ames, IA 50014; the Department of Clinical Studies, Veterinary Hospital at the University of Pennsylvania, Philadelphia, PA 19104-6010 (Brown, Smith, Washabau, LaFond); and the Research Division, Department of Obstetrics and Gynecology, Uniformed Services University of the Health Sciences, Bethesda, MD 20814-4799 (Chakraborty).

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Prabir K. Chakraborty From the Departments of Clinical Studies (Conzemius) and Statistics (Brabec), College of Veterinary Medicine, Iowa State University, Ames, IA 50014; the Department of Clinical Studies, Veterinary Hospital at the University of Pennsylvania, Philadelphia, PA 19104-6010 (Brown, Smith, Washabau, LaFond); and the Research Division, Department of Obstetrics and Gynecology, Uniformed Services University of the Health Sciences, Bethesda, MD 20814-4799 (Chakraborty).

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Abstract

Objective

To determine the association between longitudinal bone growth and concentrations of growth hormone (GH) and insulin-like growth factor-I (IGF-I) in serum from prepubertal dogs.

Animals

6 male 14-week-old German Shepherd Dogs.

Procedure

Blood was obtained every 30 minutes for 14 consecutive days. Concentrations of GH and IGF-I in serum were determined, using a canine-specific radioimmunoassay and conventional radioimmunoassay after acid-ethanol extraction, respectively. Simultaneous biplanar radiography was performed daily to measure bone growth. Spectral analysis was used to estimate specific features of GH secretion during an extended period. Multiple linear regression with different lag times between independent and dependent variables was used to determine the strongest predictors of bone growth.

Results

The power spectra of GH concentrations in serum had a primary peak at a frequency of 0.02 cycles/h or a periodicity of 50 h/cycle. A significant determinant of longitudinal bone growth was a lag time of 1 day in concentration of GH in serum. The relationship between IGF-I concentration in serum and bone growth was not significant.

Conclusions

The primary frequency of GH secretion is outside the time frame of a single day and the concentration of GH in serum is a primary determinant of bone growth.

Clinical Relevance

A better understanding of the components of bone growth provide discernment to improved diagnosis and treatment of abnormal bone growth. (Am J Vet Res 1998;59:1608-1612)

Abstract

Objective

To determine the association between longitudinal bone growth and concentrations of growth hormone (GH) and insulin-like growth factor-I (IGF-I) in serum from prepubertal dogs.

Animals

6 male 14-week-old German Shepherd Dogs.

Procedure

Blood was obtained every 30 minutes for 14 consecutive days. Concentrations of GH and IGF-I in serum were determined, using a canine-specific radioimmunoassay and conventional radioimmunoassay after acid-ethanol extraction, respectively. Simultaneous biplanar radiography was performed daily to measure bone growth. Spectral analysis was used to estimate specific features of GH secretion during an extended period. Multiple linear regression with different lag times between independent and dependent variables was used to determine the strongest predictors of bone growth.

Results

The power spectra of GH concentrations in serum had a primary peak at a frequency of 0.02 cycles/h or a periodicity of 50 h/cycle. A significant determinant of longitudinal bone growth was a lag time of 1 day in concentration of GH in serum. The relationship between IGF-I concentration in serum and bone growth was not significant.

Conclusions

The primary frequency of GH secretion is outside the time frame of a single day and the concentration of GH in serum is a primary determinant of bone growth.

Clinical Relevance

A better understanding of the components of bone growth provide discernment to improved diagnosis and treatment of abnormal bone growth. (Am J Vet Res 1998;59:1608-1612)

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