Hematologic and serum biochemical variables and plasma corticotropin concentration in healthy aged horses

Dianne McFarlane From the Departments of Food Animal and Equine Medicine and Anatomy (McFarlane, Sellon, Gibbs), Physiological Sciences, and Radiology (Gaffney, Hedgpeth, Papich), College of Veterinary Medicine, North Carolina State University, Raleigh, NC 27606.

Search for other papers by Dianne McFarlane in
Current site
Google Scholar
PubMed
Close
 DVM, MS
,
Debra C. Sellon From the Departments of Food Animal and Equine Medicine and Anatomy (McFarlane, Sellon, Gibbs), Physiological Sciences, and Radiology (Gaffney, Hedgpeth, Papich), College of Veterinary Medicine, North Carolina State University, Raleigh, NC 27606.

Search for other papers by Debra C. Sellon in
Current site
Google Scholar
PubMed
Close
 DVM, PhD
,
Debbie Gaffney From the Departments of Food Animal and Equine Medicine and Anatomy (McFarlane, Sellon, Gibbs), Physiological Sciences, and Radiology (Gaffney, Hedgpeth, Papich), College of Veterinary Medicine, North Carolina State University, Raleigh, NC 27606.

Search for other papers by Debbie Gaffney in
Current site
Google Scholar
PubMed
Close
,
Vickie Hedgpeth From the Departments of Food Animal and Equine Medicine and Anatomy (McFarlane, Sellon, Gibbs), Physiological Sciences, and Radiology (Gaffney, Hedgpeth, Papich), College of Veterinary Medicine, North Carolina State University, Raleigh, NC 27606.

Search for other papers by Vickie Hedgpeth in
Current site
Google Scholar
PubMed
Close
 BS
,
Mark Papich From the Departments of Food Animal and Equine Medicine and Anatomy (McFarlane, Sellon, Gibbs), Physiological Sciences, and Radiology (Gaffney, Hedgpeth, Papich), College of Veterinary Medicine, North Carolina State University, Raleigh, NC 27606.

Search for other papers by Mark Papich in
Current site
Google Scholar
PubMed
Close
 DVM, MS
, and
Scott Gibbs From the Departments of Food Animal and Equine Medicine and Anatomy (McFarlane, Sellon, Gibbs), Physiological Sciences, and Radiology (Gaffney, Hedgpeth, Papich), College of Veterinary Medicine, North Carolina State University, Raleigh, NC 27606.

Search for other papers by Scott Gibbs in
Current site
Google Scholar
PubMed
Close
 BS

Abstract

Objectives

To compare hematologic and serum biochemical variables and plasma ACTH concentration between healthy horses 5 to 12 years old and those more than 20 years old.

Animals

30 healthy horses 5 to 12 years old and 30 healthy horses more than 20 years old.

Procedures

Venous blood was collected from all horses, and CBC and serum biochemical analysis were performed for each horse. Plasma ACTH concentration was determined by radioimmunoassay. Student's paired t-test or the Mann-Whitney rank sum test was used to compare values between control and aged horse groups.

Results

Compared with values for control horses, aged horses had significantly higher erythrocyte mean cell volume and mean cell hemoglobin. Aged horses also had significantly decreased total lymphocyte count. Five aged horses had lymphocyte count that was lower than the low reference limit as established for horses in our laboratory. Differences between control and aged horses for serum biochemical or plasma ACTH values were not significant.

Conclusion

Compared with younger adult horses, those more than 20 years old have some hematologic differences, but there is no apparent effect of aging on baseline plasma ACTH concentration.

Clinical Relevance

It is important to establish age-matched control values for optimal interpretation of clinicopathologic variables. (Am J Vet Res 1998;59: 1247–1251)

Abstract

Objectives

To compare hematologic and serum biochemical variables and plasma ACTH concentration between healthy horses 5 to 12 years old and those more than 20 years old.

Animals

30 healthy horses 5 to 12 years old and 30 healthy horses more than 20 years old.

Procedures

Venous blood was collected from all horses, and CBC and serum biochemical analysis were performed for each horse. Plasma ACTH concentration was determined by radioimmunoassay. Student's paired t-test or the Mann-Whitney rank sum test was used to compare values between control and aged horse groups.

Results

Compared with values for control horses, aged horses had significantly higher erythrocyte mean cell volume and mean cell hemoglobin. Aged horses also had significantly decreased total lymphocyte count. Five aged horses had lymphocyte count that was lower than the low reference limit as established for horses in our laboratory. Differences between control and aged horses for serum biochemical or plasma ACTH values were not significant.

Conclusion

Compared with younger adult horses, those more than 20 years old have some hematologic differences, but there is no apparent effect of aging on baseline plasma ACTH concentration.

Clinical Relevance

It is important to establish age-matched control values for optimal interpretation of clinicopathologic variables. (Am J Vet Res 1998;59: 1247–1251)

Advertisement