Morphometric analysis of endometrial periglandular fibrosis in mares

T. J. Evans From the Departments of Veterinary Medicine and Surgery (Evans, Youngquist), Veterinary Pathobiology (Miller), Veterinary Biomedical Sciences (Ganjam, Niswender), and Pathology and Anatomical Sciences (Krause) and the Agricultural Experiment Station (Ellersieck), University of Missouri, Columbia, MO 65211.

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M. A. Miller From the Departments of Veterinary Medicine and Surgery (Evans, Youngquist), Veterinary Pathobiology (Miller), Veterinary Biomedical Sciences (Ganjam, Niswender), and Pathology and Anatomical Sciences (Krause) and the Agricultural Experiment Station (Ellersieck), University of Missouri, Columbia, MO 65211.

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V. K. Ganjam From the Departments of Veterinary Medicine and Surgery (Evans, Youngquist), Veterinary Pathobiology (Miller), Veterinary Biomedical Sciences (Ganjam, Niswender), and Pathology and Anatomical Sciences (Krause) and the Agricultural Experiment Station (Ellersieck), University of Missouri, Columbia, MO 65211.

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K. D. Niswender From the Departments of Veterinary Medicine and Surgery (Evans, Youngquist), Veterinary Pathobiology (Miller), Veterinary Biomedical Sciences (Ganjam, Niswender), and Pathology and Anatomical Sciences (Krause) and the Agricultural Experiment Station (Ellersieck), University of Missouri, Columbia, MO 65211.

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M. R. Ellersieck From the Departments of Veterinary Medicine and Surgery (Evans, Youngquist), Veterinary Pathobiology (Miller), Veterinary Biomedical Sciences (Ganjam, Niswender), and Pathology and Anatomical Sciences (Krause) and the Agricultural Experiment Station (Ellersieck), University of Missouri, Columbia, MO 65211.

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W. J. Krause From the Departments of Veterinary Medicine and Surgery (Evans, Youngquist), Veterinary Pathobiology (Miller), Veterinary Biomedical Sciences (Ganjam, Niswender), and Pathology and Anatomical Sciences (Krause) and the Agricultural Experiment Station (Ellersieck), University of Missouri, Columbia, MO 65211.

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R. S. Youngquist From the Departments of Veterinary Medicine and Surgery (Evans, Youngquist), Veterinary Pathobiology (Miller), Veterinary Biomedical Sciences (Ganjam, Niswender), and Pathology and Anatomical Sciences (Krause) and the Agricultural Experiment Station (Ellersieck), University of Missouri, Columbia, MO 65211.

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Abstract

Objectives

To develop an objective, quantifiable assay for endometrial periglandular fibrosis (EPF) and correlate assay results with histologic and ultrastructural changes in equine endometrial biopsy specimens.

Sample Population

Endometrial biopsy specimens from 70 mares from 3 to 27 years old in estrus.

Procedure

In a double-blinded study design, endometrial biopsy specimens were graded histologically (modified Kenney classification) for EPF and inflammation. Endometrial periglandular collagen volume fraction (%EPCVF) was determined by light microscopic image analysis of picrosirius red-stained sections. Specimens from selected mares were examined by transmission electron microscopy.

Results

%EPCVF values varied significantly among the 4 modified Kenney EPF categories (I, IIA, IIB, and III) and increased with increasing age of mares. Morphologically, EPF consisted of concentric layers of transformed fibroblasts with myofibroblastic features and deposition of fibrillar collagen around unaltered glandular basal laminae.

Conclusions and Clinical Relevance

%EPCVF correlates well with morphologic changes in endometrial biopsy specimens. Determination of %EPCVF could be useful in evaluation and clinical management of subfertile mares and in investigations of the pathogenesis of EPF. (Am J Vet Res 1998;59:1209-1214)

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Cover American Journal of Veterinary Research
American Journal of Veterinary Research
Issue:
01 Oct 1998
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