Cell trafficking, mediator release, and articular metabolism in acute inflammation of innervated or denervated isolated equine joints

Joanne Hardy From the Departments of Veterinary Clinical Sciences (Hardy, Bertone, Muir), Veterinary Biosciences (Weisbrode, Masty), and Internal Medicine (O'Dorisio), The Ohio State University, Columbus, OH 43210.

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Alicia L. Bertone From the Departments of Veterinary Clinical Sciences (Hardy, Bertone, Muir), Veterinary Biosciences (Weisbrode, Masty), and Internal Medicine (O'Dorisio), The Ohio State University, Columbus, OH 43210.

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Steven E. Weisbrode From the Departments of Veterinary Clinical Sciences (Hardy, Bertone, Muir), Veterinary Biosciences (Weisbrode, Masty), and Internal Medicine (O'Dorisio), The Ohio State University, Columbus, OH 43210.

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William W. Muir From the Departments of Veterinary Clinical Sciences (Hardy, Bertone, Muir), Veterinary Biosciences (Weisbrode, Masty), and Internal Medicine (O'Dorisio), The Ohio State University, Columbus, OH 43210.

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Thomas M. O'Dorisio From the Departments of Veterinary Clinical Sciences (Hardy, Bertone, Muir), Veterinary Biosciences (Weisbrode, Masty), and Internal Medicine (O'Dorisio), The Ohio State University, Columbus, OH 43210.

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Jerome Masty From the Departments of Veterinary Clinical Sciences (Hardy, Bertone, Muir), Veterinary Biosciences (Weisbrode, Masty), and Internal Medicine (O'Dorisio), The Ohio State University, Columbus, OH 43210.

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SUMMARY

Objectives

To describe the acute cellular response, inflammatory mediator release, and effect on chondrocyte metabolism of interleukin 1β (IL-1β) in isolated innervated or denervated equine metacarpophalangeal joints.

Animals

One metacarpophalangeal joint of 24 adult horses.

Procedures

The metacarpophalangeal joint was isolated for 6 hours in a pump-perfused, auto-oxygenated, innervated or denervated metacarpophalangeal joint preparation. Isolated joints were assigned to 4 groups: control, control-denervated, inflamed, and inflamed-denervated, and inflammation was induced by intra-articular injection of IL-1β. Synovial fluid was collected for cytologic examination and determination of IL (IL)-1β, (IL-6), prostaglandin E2 (PGE2), and substance P (SP) values. Synovial membrane was immunostained with SP and nerve-specific enolase (NSE) antibodies. Cartilage was collected for determination of proteoglycan (PG) synthesis and degradation.

Results

IL-1β induced significant neutrophilic leukocytosis in synovial fluid and synovial membrane. IL-1β concentration had returned to baseline by 5.5 hours, but IL-6 concentration significantly increased throughout the study. Total SP content was significantly higher in inflamed joints. There was a significant increase in 24- and 48-hour PG degradation in inflamed innervated joints.

Conclusion

Cellular response to IL-1β was rapid and sustained; joint clearance of IL-1β was rapid, and endogenous production of IL-1β did not follow. The IL-6 and PGE2 concentrations significantly increased, and SP content was increased in association with inflammation but not denervation. A degradative response of cartilage to IL-1β was observed, and was enhanced by innervation. This model was useful for investigation of the articular response to acute inflammation and the influence of denervation in modulating this response. (Am J Vet Res 1998;59:88–100)

SUMMARY

Objectives

To describe the acute cellular response, inflammatory mediator release, and effect on chondrocyte metabolism of interleukin 1β (IL-1β) in isolated innervated or denervated equine metacarpophalangeal joints.

Animals

One metacarpophalangeal joint of 24 adult horses.

Procedures

The metacarpophalangeal joint was isolated for 6 hours in a pump-perfused, auto-oxygenated, innervated or denervated metacarpophalangeal joint preparation. Isolated joints were assigned to 4 groups: control, control-denervated, inflamed, and inflamed-denervated, and inflammation was induced by intra-articular injection of IL-1β. Synovial fluid was collected for cytologic examination and determination of IL (IL)-1β, (IL-6), prostaglandin E2 (PGE2), and substance P (SP) values. Synovial membrane was immunostained with SP and nerve-specific enolase (NSE) antibodies. Cartilage was collected for determination of proteoglycan (PG) synthesis and degradation.

Results

IL-1β induced significant neutrophilic leukocytosis in synovial fluid and synovial membrane. IL-1β concentration had returned to baseline by 5.5 hours, but IL-6 concentration significantly increased throughout the study. Total SP content was significantly higher in inflamed joints. There was a significant increase in 24- and 48-hour PG degradation in inflamed innervated joints.

Conclusion

Cellular response to IL-1β was rapid and sustained; joint clearance of IL-1β was rapid, and endogenous production of IL-1β did not follow. The IL-6 and PGE2 concentrations significantly increased, and SP content was increased in association with inflammation but not denervation. A degradative response of cartilage to IL-1β was observed, and was enhanced by innervation. This model was useful for investigation of the articular response to acute inflammation and the influence of denervation in modulating this response. (Am J Vet Res 1998;59:88–100)

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