Effect of combined administration of insulin-like growth factor and platelet-derived growth factor on the regeneration of transected and anastomosed sciatic nerve in rats

Janet A. Welch From the School of Veterinary Medicine, Tufts University, 200 Westboro Rd, North Grafton, MA 01536 (Welch, Kraus), and The Institute of Molecular Biology, street address, Worcester, MA zip code (Wells, Blunt, Weremowitz).

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Karl H. Kraus From the School of Veterinary Medicine, Tufts University, 200 Westboro Rd, North Grafton, MA 01536 (Welch, Kraus), and The Institute of Molecular Biology, street address, Worcester, MA zip code (Wells, Blunt, Weremowitz).

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Michael R. Wells From the School of Veterinary Medicine, Tufts University, 200 Westboro Rd, North Grafton, MA 01536 (Welch, Kraus), and The Institute of Molecular Biology, street address, Worcester, MA zip code (Wells, Blunt, Weremowitz).

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Dana G. Blunt From the School of Veterinary Medicine, Tufts University, 200 Westboro Rd, North Grafton, MA 01536 (Welch, Kraus), and The Institute of Molecular Biology, street address, Worcester, MA zip code (Wells, Blunt, Weremowitz).

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J. Weremowitz From the School of Veterinary Medicine, Tufts University, 200 Westboro Rd, North Grafton, MA 01536 (Welch, Kraus), and The Institute of Molecular Biology, street address, Worcester, MA zip code (Wells, Blunt, Weremowitz).

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Abstract

Objective

To evaluate the efficacy of combined administration of platelet-derived growth factor (PDGF) and insulin-like growth factor (IGF-I) on the regeneration of transected and anastomosed sciatic nerves.

Animals

25 male Lewis rats.

Procedure

Transection and anastomosis was performed of the left sciatic nerve of all rats. At the time of surgery, treatment group rats received a combination of PDGF (0.75 μg) and IGF-I (1.5 μg) along with vehicle (2% methyl cellulose gel) at the anastomosis site. Control rats received vehicle alone. Evaluation consisted of motor evoked potentials and nerve conduction velocity, quantitative histologic examination, sciatic functional index, and visual assessment of repair organization (lesion scores).

Results

Significant differences in motor evoked potential latency, nerve conduction velocity, axon number, sciatic functional index, or lesion scores were not found between groups.

Conclusions

Combined administration of PDGF and IGF-I did not enhance peripheral nerve regeneration in a transection and anastomosis model as determined 6 weeks after surgery.

Clinical Relevance

These findings support the assumption that mechanical, versus trophic, effects predominate when nerve ends are closely apposed. (Am J Vet Res 1997;58:1033–1037)

Abstract

Objective

To evaluate the efficacy of combined administration of platelet-derived growth factor (PDGF) and insulin-like growth factor (IGF-I) on the regeneration of transected and anastomosed sciatic nerves.

Animals

25 male Lewis rats.

Procedure

Transection and anastomosis was performed of the left sciatic nerve of all rats. At the time of surgery, treatment group rats received a combination of PDGF (0.75 μg) and IGF-I (1.5 μg) along with vehicle (2% methyl cellulose gel) at the anastomosis site. Control rats received vehicle alone. Evaluation consisted of motor evoked potentials and nerve conduction velocity, quantitative histologic examination, sciatic functional index, and visual assessment of repair organization (lesion scores).

Results

Significant differences in motor evoked potential latency, nerve conduction velocity, axon number, sciatic functional index, or lesion scores were not found between groups.

Conclusions

Combined administration of PDGF and IGF-I did not enhance peripheral nerve regeneration in a transection and anastomosis model as determined 6 weeks after surgery.

Clinical Relevance

These findings support the assumption that mechanical, versus trophic, effects predominate when nerve ends are closely apposed. (Am J Vet Res 1997;58:1033–1037)

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