Effects of tromethamine buffer on coagulation variables and ionized calcium concentration in dogs

P. F. Moon From the Department of Clinical Sciences, College of Veterinary Medicine, Cornell University, Ithaca, NY 14850.

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S. C. Barr From the Department of Clinical Sciences, College of Veterinary Medicine, Cornell University, Ithaca, NY 14850.

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H. N. Erb From the Department of Clinical Sciences, College of Veterinary Medicine, Cornell University, Ithaca, NY 14850.

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Abstract

Objective

To evaluate coagulation variables in 2 groups of dogs after tromethamine administration.

Animals

13 Beagles.

Procedures

Both groups of dogs received a 30-minute IV infusion of 10 ml of 0.3M tromethamine/kg of body weight. In unsedated dogs (group 1, n = 8), prothrombin time, activated partial thromboplastin time, normalized ionized calcium concentration, platelet numbers, and platelet function were measured prior to treatment, at the end of the infusion, and 1 hour after the infusion. In xylazine-sedated dogs (group 2, n = 5), buccal mucosal bleeding time and plasma percentage of von Willebrand factor antigen were measured before and 1 hour after infusion, and fibrin degradation products concentration was measured 1 hour after infusion. Platelet function was assessed by determining platelet aggregation and by measuring ATP release from the aggregating platelets over 6 minutes, using a whole blood aggregometer, with 20, 10, and 5 μM ADP and 5 and 10 μg of collagen/ml as platelet activation agonists.

Results

There was no significant change in any of the variables measured in either group of dogs, compared with baseline values.

Conclusions and Clinical Relevance

When administered to healthy dogs, tromethamine does not change the coagulation indices measured. (Am J Vet Res 1997;58:777–780)

Abstract

Objective

To evaluate coagulation variables in 2 groups of dogs after tromethamine administration.

Animals

13 Beagles.

Procedures

Both groups of dogs received a 30-minute IV infusion of 10 ml of 0.3M tromethamine/kg of body weight. In unsedated dogs (group 1, n = 8), prothrombin time, activated partial thromboplastin time, normalized ionized calcium concentration, platelet numbers, and platelet function were measured prior to treatment, at the end of the infusion, and 1 hour after the infusion. In xylazine-sedated dogs (group 2, n = 5), buccal mucosal bleeding time and plasma percentage of von Willebrand factor antigen were measured before and 1 hour after infusion, and fibrin degradation products concentration was measured 1 hour after infusion. Platelet function was assessed by determining platelet aggregation and by measuring ATP release from the aggregating platelets over 6 minutes, using a whole blood aggregometer, with 20, 10, and 5 μM ADP and 5 and 10 μg of collagen/ml as platelet activation agonists.

Results

There was no significant change in any of the variables measured in either group of dogs, compared with baseline values.

Conclusions and Clinical Relevance

When administered to healthy dogs, tromethamine does not change the coagulation indices measured. (Am J Vet Res 1997;58:777–780)

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