Identification of β-adrenergic receptor subtypes mediating relaxation in isolated equine ileum

Giovanni Re From the Department of Animal Pathology, Division of Pharmacology and Toxicology, University of Turin, Via Nizza 52-10126 Turin, Italy (Re, Badino, Novelli, Girardi), and the Institute of Veterinary Pharmacology and Toxicology, University of Milan, Via Celoria 10-20133 Milan, Italy (Belloli, Arioli, Beretta).

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Chiara Belloli From the Department of Animal Pathology, Division of Pharmacology and Toxicology, University of Turin, Via Nizza 52-10126 Turin, Italy (Re, Badino, Novelli, Girardi), and the Institute of Veterinary Pharmacology and Toxicology, University of Milan, Via Celoria 10-20133 Milan, Italy (Belloli, Arioli, Beretta).

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Paola Badino From the Department of Animal Pathology, Division of Pharmacology and Toxicology, University of Turin, Via Nizza 52-10126 Turin, Italy (Re, Badino, Novelli, Girardi), and the Institute of Veterinary Pharmacology and Toxicology, University of Milan, Via Celoria 10-20133 Milan, Italy (Belloli, Arioli, Beretta).

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Francesco Arioli From the Department of Animal Pathology, Division of Pharmacology and Toxicology, University of Turin, Via Nizza 52-10126 Turin, Italy (Re, Badino, Novelli, Girardi), and the Institute of Veterinary Pharmacology and Toxicology, University of Milan, Via Celoria 10-20133 Milan, Italy (Belloli, Arioli, Beretta).

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Antonella Novelli From the Department of Animal Pathology, Division of Pharmacology and Toxicology, University of Turin, Via Nizza 52-10126 Turin, Italy (Re, Badino, Novelli, Girardi), and the Institute of Veterinary Pharmacology and Toxicology, University of Milan, Via Celoria 10-20133 Milan, Italy (Belloli, Arioli, Beretta).

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Carlo Girardi From the Department of Animal Pathology, Division of Pharmacology and Toxicology, University of Turin, Via Nizza 52-10126 Turin, Italy (Re, Badino, Novelli, Girardi), and the Institute of Veterinary Pharmacology and Toxicology, University of Milan, Via Celoria 10-20133 Milan, Italy (Belloli, Arioli, Beretta).

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Carlo Beretta From the Department of Animal Pathology, Division of Pharmacology and Toxicology, University of Turin, Via Nizza 52-10126 Turin, Italy (Re, Badino, Novelli, Girardi), and the Institute of Veterinary Pharmacology and Toxicology, University of Milan, Via Celoria 10-20133 Milan, Italy (Belloli, Arioli, Beretta).

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Abstract

Objective

To identify β-adrenergic receptor subtypes in ileum smooth muscle of the horse.

Sample Population

Isolated strips of equine longitudinal ileum smooth muscle and membrane preparations from smooth muscle of the intestinal wall.

Procedure

Functional assays and radioligand binding assays.

Results

Relaxation of ileum longitudinal smooth muscle proved to be mainly caused by stimulation of β-atypical and β2-adrenergic receptors. Binding studies on cell membranes indicated that the total β-adrenergic receptors population consists of 54% β-atypical, 34% β2-, and 12% β1-subtypes.

Conclusions

The data suggest that sympathetic relaxation of equine ileum smooth muscle depends mainly on β-atypical receptor subtypes activation, with a minor contribution by β2-subtypes.

Clinical Relevance

The important role of β-atypical adrenergic receptor subtypes in the relaxation of equine ileum suggests possible clinical use of selective β-atypical receptor agonists to control intestinal disturbances. (Am J Vet Res 1997;58:621–625)

Abstract

Objective

To identify β-adrenergic receptor subtypes in ileum smooth muscle of the horse.

Sample Population

Isolated strips of equine longitudinal ileum smooth muscle and membrane preparations from smooth muscle of the intestinal wall.

Procedure

Functional assays and radioligand binding assays.

Results

Relaxation of ileum longitudinal smooth muscle proved to be mainly caused by stimulation of β-atypical and β2-adrenergic receptors. Binding studies on cell membranes indicated that the total β-adrenergic receptors population consists of 54% β-atypical, 34% β2-, and 12% β1-subtypes.

Conclusions

The data suggest that sympathetic relaxation of equine ileum smooth muscle depends mainly on β-atypical receptor subtypes activation, with a minor contribution by β2-subtypes.

Clinical Relevance

The important role of β-atypical adrenergic receptor subtypes in the relaxation of equine ileum suggests possible clinical use of selective β-atypical receptor agonists to control intestinal disturbances. (Am J Vet Res 1997;58:621–625)

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