Pathogenesis of infection induced by an adenovirus isolated from a goat

H. D. Lehmkuhl From the Respiratory and Neurologic Disease Research Unit, National Animal Disease Center, Agricultural Research Service, United States Department of Agriculture, PO Box 70, Ames, IA 50010.

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R. C. Cutlip From the Respiratory and Neurologic Disease Research Unit, National Animal Disease Center, Agricultural Research Service, United States Department of Agriculture, PO Box 70, Ames, IA 50010.

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J. T. Meehan From the Respiratory and Neurologic Disease Research Unit, National Animal Disease Center, Agricultural Research Service, United States Department of Agriculture, PO Box 70, Ames, IA 50010.

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B. M. DeBey From the Respiratory and Neurologic Disease Research Unit, National Animal Disease Center, Agricultural Research Service, United States Department of Agriculture, PO Box 70, Ames, IA 50010.

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Abstract

Objective

To determine the pathogenic potential of an adenovirus isolated from a goat.

Animals

14 colostrum-deprived, isolation-reared goat kids approximately 3 weeks old.

Procedure

Kids were inoculated with either cell culture fluid containing adenovirus (n = 10) or uninfected cell culture fluid (n = 4): 2 ml transtracheally and 1 ml/nostril. Clinical signs of disease and rectal temperature were recorded daily; nasal secretion and fecal specimens were collected daily. Control kids were necropsied, 2/d, on postinoculation days (PID) 5 and 10. Virus-inoculated kids were necropsied on PID 3, 5, 7, 10, and 28. After necropsy, lung, liver, kidney, and brain specimens were aseptically collected for virus isolation attempts. Tracheal fluid was collected on sterile cotton swabs. Turbinate, trachea, lung, mediastinal lymph node, liver, kidney, duodenum, jejunum, ileum, mesenteric lymph node, colon, and brain specimens were collected for histologic evaluation.

Results

Kids developed mild-to-moderate clinical respiratory tract infection. Virus was recovered consistently from nasal secretion and sporadically from fecal specimens. Grossly, there were multiple areas of atelectasis and hyperemia, principally in the cranioventral portion of the lungs. Microscopically, there was detachment and sloughing of foci of epithelial cells of the terminal bronchioles and alveoli. In kids necropsied late in the disease, these changes were accompanied by hyperplasia of type-II epithelial cells. Viral inclusions were not an obvious feature, but a few cells contained probable inclusions.

Conclusions and Clinical Relevance

The caprine adenovirus reported here is capable of inducing respiratory tract disease and lesions in the lungs of young kids. (Am J Vet Res 1997;58:608–611)

Abstract

Objective

To determine the pathogenic potential of an adenovirus isolated from a goat.

Animals

14 colostrum-deprived, isolation-reared goat kids approximately 3 weeks old.

Procedure

Kids were inoculated with either cell culture fluid containing adenovirus (n = 10) or uninfected cell culture fluid (n = 4): 2 ml transtracheally and 1 ml/nostril. Clinical signs of disease and rectal temperature were recorded daily; nasal secretion and fecal specimens were collected daily. Control kids were necropsied, 2/d, on postinoculation days (PID) 5 and 10. Virus-inoculated kids were necropsied on PID 3, 5, 7, 10, and 28. After necropsy, lung, liver, kidney, and brain specimens were aseptically collected for virus isolation attempts. Tracheal fluid was collected on sterile cotton swabs. Turbinate, trachea, lung, mediastinal lymph node, liver, kidney, duodenum, jejunum, ileum, mesenteric lymph node, colon, and brain specimens were collected for histologic evaluation.

Results

Kids developed mild-to-moderate clinical respiratory tract infection. Virus was recovered consistently from nasal secretion and sporadically from fecal specimens. Grossly, there were multiple areas of atelectasis and hyperemia, principally in the cranioventral portion of the lungs. Microscopically, there was detachment and sloughing of foci of epithelial cells of the terminal bronchioles and alveoli. In kids necropsied late in the disease, these changes were accompanied by hyperplasia of type-II epithelial cells. Viral inclusions were not an obvious feature, but a few cells contained probable inclusions.

Conclusions and Clinical Relevance

The caprine adenovirus reported here is capable of inducing respiratory tract disease and lesions in the lungs of young kids. (Am J Vet Res 1997;58:608–611)

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