Detection of antibodies to Aspergillus fumigatus in serum of horses with mycosis of the auditory tube diverticulum (guttural pouch)

Jacques Guillot From Equipe de Parasitologie-Mycologie, URA-INRA-DGER Immunopathologie Cellulaire et Moléculaire, Ecole Nationale Vétérinaire d'Alfort, 7, avenue du Général de Gaulle, 94704 Maisons-Alfort Cedex, France (Guillot); Laboratoire des Aspergillus, Institut Pasteur, 25, rue du Docteur Roux, 75724 Paris Cedex 15, France (Sarfati, Latgé); Groupe de Secteurs Vétérinaires de Paris, Ecole Militaire, 1, place Joffre, 75700 Paris, France (Ribot); and the Laboratory of Veterinary Pathology, The Royal Veterinary and Agricultural University, DK-1870 Frederiksberg C, Copenhagen, Denmark (Jensen).

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Jacqueline Sarfati From Equipe de Parasitologie-Mycologie, URA-INRA-DGER Immunopathologie Cellulaire et Moléculaire, Ecole Nationale Vétérinaire d'Alfort, 7, avenue du Général de Gaulle, 94704 Maisons-Alfort Cedex, France (Guillot); Laboratoire des Aspergillus, Institut Pasteur, 25, rue du Docteur Roux, 75724 Paris Cedex 15, France (Sarfati, Latgé); Groupe de Secteurs Vétérinaires de Paris, Ecole Militaire, 1, place Joffre, 75700 Paris, France (Ribot); and the Laboratory of Veterinary Pathology, The Royal Veterinary and Agricultural University, DK-1870 Frederiksberg C, Copenhagen, Denmark (Jensen).

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Xavier Ribot From Equipe de Parasitologie-Mycologie, URA-INRA-DGER Immunopathologie Cellulaire et Moléculaire, Ecole Nationale Vétérinaire d'Alfort, 7, avenue du Général de Gaulle, 94704 Maisons-Alfort Cedex, France (Guillot); Laboratoire des Aspergillus, Institut Pasteur, 25, rue du Docteur Roux, 75724 Paris Cedex 15, France (Sarfati, Latgé); Groupe de Secteurs Vétérinaires de Paris, Ecole Militaire, 1, place Joffre, 75700 Paris, France (Ribot); and the Laboratory of Veterinary Pathology, The Royal Veterinary and Agricultural University, DK-1870 Frederiksberg C, Copenhagen, Denmark (Jensen).

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Henrik E. Jensen From Equipe de Parasitologie-Mycologie, URA-INRA-DGER Immunopathologie Cellulaire et Moléculaire, Ecole Nationale Vétérinaire d'Alfort, 7, avenue du Général de Gaulle, 94704 Maisons-Alfort Cedex, France (Guillot); Laboratoire des Aspergillus, Institut Pasteur, 25, rue du Docteur Roux, 75724 Paris Cedex 15, France (Sarfati, Latgé); Groupe de Secteurs Vétérinaires de Paris, Ecole Militaire, 1, place Joffre, 75700 Paris, France (Ribot); and the Laboratory of Veterinary Pathology, The Royal Veterinary and Agricultural University, DK-1870 Frederiksberg C, Copenhagen, Denmark (Jensen).

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Jean-Paul Latgé From Equipe de Parasitologie-Mycologie, URA-INRA-DGER Immunopathologie Cellulaire et Moléculaire, Ecole Nationale Vétérinaire d'Alfort, 7, avenue du Général de Gaulle, 94704 Maisons-Alfort Cedex, France (Guillot); Laboratoire des Aspergillus, Institut Pasteur, 25, rue du Docteur Roux, 75724 Paris Cedex 15, France (Sarfati, Latgé); Groupe de Secteurs Vétérinaires de Paris, Ecole Militaire, 1, place Joffre, 75700 Paris, France (Ribot); and the Laboratory of Veterinary Pathology, The Royal Veterinary and Agricultural University, DK-1870 Frederiksberg C, Copenhagen, Denmark (Jensen).

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DOI:
https://doi.org/10.2460/ajvr.1997.58.12.1364
Volume/Issue:
Volume 58: Issue 12
Received:
18 Feb 1997
|
Accepted:
19 May 1997
|
Online Publication Date:
01 Dec 1997
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SUMMARY

Objective

To detect antibodies against Aspergillus fumigatus antigens in serum samples from horses and to evaluate the relevance of this method as an alternative approach to the diagnosis of mycosis of the auditory tube diverticulum (guttural pouch mycosis [GPM]).

Animals

Twelve clinically normal horses (controls) and 12 horses with GPM diagnosed by endoscopic observation of characteristic mycotic plaques.

Procedure

Antibodies to A fumigatus antigens were detected in serum by use of an ELISA and immunoblot analysis with extracellular antigens.

Results

Antibodies against A fumigatus antigens were found in healthy and diseased horses. Titer of total Aspergillus antibodies was not diagnostic for GPM. In contrast, immunoblot analysis results indicated that 2 antigens of 22 and 26 kd were constantly recognized by sera from diseased horses.

Conclusions

Reactivity to 22- and 26-kd A fumigatus antigens, as measured by immunoblot analysis, seemed to be diagnostic for GPM in horses. (Am J Vet Res 1997;58:1364–1366)

SUMMARY

Objective

To detect antibodies against Aspergillus fumigatus antigens in serum samples from horses and to evaluate the relevance of this method as an alternative approach to the diagnosis of mycosis of the auditory tube diverticulum (guttural pouch mycosis [GPM]).

Animals

Twelve clinically normal horses (controls) and 12 horses with GPM diagnosed by endoscopic observation of characteristic mycotic plaques.

Procedure

Antibodies to A fumigatus antigens were detected in serum by use of an ELISA and immunoblot analysis with extracellular antigens.

Results

Antibodies against A fumigatus antigens were found in healthy and diseased horses. Titer of total Aspergillus antibodies was not diagnostic for GPM. In contrast, immunoblot analysis results indicated that 2 antigens of 22 and 26 kd were constantly recognized by sera from diseased horses.

Conclusions

Reactivity to 22- and 26-kd A fumigatus antigens, as measured by immunoblot analysis, seemed to be diagnostic for GPM in horses. (Am J Vet Res 1997;58:1364–1366)

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Cover American Journal of Veterinary Research
American Journal of Veterinary Research
Publication Date:
01 Dec 1997
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