Editorial Type:
Immunology

Effects of Pasteurella haemolytica leukotoxin and lipopolysaccharide on histamine, prostanoid, and leukotriene release by bovine lung parenchyma in vitro

Ricardo Saban From the Department of Surgical Sciences, School of Veterinary Medicine, University of Wisconsin, Madison, WI 53706 (Saban, Skoyen, Fialkowski, Bjorling); the Department of Small Animal Clinical Sciences, Virginia-Maryland Regional College of Veterinary Medicine, Blacksburg, VA 24061-0442 (Broadstone); Promega Corp, Madison, WI 53711 (Haak-Frendscho); the Department of Veterinary Pathobiology, College of Veterinary Medicine, St Paul, MN 55108 (Maheswaran); and the Department of Pathobiological Sciences, School of Veterinary Medicine, University of Wisconsin, Madison, WI 53706 (Czuprynski).

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Richard V. Broadstone From the Department of Surgical Sciences, School of Veterinary Medicine, University of Wisconsin, Madison, WI 53706 (Saban, Skoyen, Fialkowski, Bjorling); the Department of Small Animal Clinical Sciences, Virginia-Maryland Regional College of Veterinary Medicine, Blacksburg, VA 24061-0442 (Broadstone); Promega Corp, Madison, WI 53711 (Haak-Frendscho); the Department of Veterinary Pathobiology, College of Veterinary Medicine, St Paul, MN 55108 (Maheswaran); and the Department of Pathobiological Sciences, School of Veterinary Medicine, University of Wisconsin, Madison, WI 53706 (Czuprynski).

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Mary Haak-Frendscho From the Department of Surgical Sciences, School of Veterinary Medicine, University of Wisconsin, Madison, WI 53706 (Saban, Skoyen, Fialkowski, Bjorling); the Department of Small Animal Clinical Sciences, Virginia-Maryland Regional College of Veterinary Medicine, Blacksburg, VA 24061-0442 (Broadstone); Promega Corp, Madison, WI 53711 (Haak-Frendscho); the Department of Veterinary Pathobiology, College of Veterinary Medicine, St Paul, MN 55108 (Maheswaran); and the Department of Pathobiological Sciences, School of Veterinary Medicine, University of Wisconsin, Madison, WI 53706 (Czuprynski).

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Sarah Skoyen From the Department of Surgical Sciences, School of Veterinary Medicine, University of Wisconsin, Madison, WI 53706 (Saban, Skoyen, Fialkowski, Bjorling); the Department of Small Animal Clinical Sciences, Virginia-Maryland Regional College of Veterinary Medicine, Blacksburg, VA 24061-0442 (Broadstone); Promega Corp, Madison, WI 53711 (Haak-Frendscho); the Department of Veterinary Pathobiology, College of Veterinary Medicine, St Paul, MN 55108 (Maheswaran); and the Department of Pathobiological Sciences, School of Veterinary Medicine, University of Wisconsin, Madison, WI 53706 (Czuprynski).

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James Fialkowski From the Department of Surgical Sciences, School of Veterinary Medicine, University of Wisconsin, Madison, WI 53706 (Saban, Skoyen, Fialkowski, Bjorling); the Department of Small Animal Clinical Sciences, Virginia-Maryland Regional College of Veterinary Medicine, Blacksburg, VA 24061-0442 (Broadstone); Promega Corp, Madison, WI 53711 (Haak-Frendscho); the Department of Veterinary Pathobiology, College of Veterinary Medicine, St Paul, MN 55108 (Maheswaran); and the Department of Pathobiological Sciences, School of Veterinary Medicine, University of Wisconsin, Madison, WI 53706 (Czuprynski).

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Sam K. Maheswaran From the Department of Surgical Sciences, School of Veterinary Medicine, University of Wisconsin, Madison, WI 53706 (Saban, Skoyen, Fialkowski, Bjorling); the Department of Small Animal Clinical Sciences, Virginia-Maryland Regional College of Veterinary Medicine, Blacksburg, VA 24061-0442 (Broadstone); Promega Corp, Madison, WI 53711 (Haak-Frendscho); the Department of Veterinary Pathobiology, College of Veterinary Medicine, St Paul, MN 55108 (Maheswaran); and the Department of Pathobiological Sciences, School of Veterinary Medicine, University of Wisconsin, Madison, WI 53706 (Czuprynski).

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Dale E. Bjorling From the Department of Surgical Sciences, School of Veterinary Medicine, University of Wisconsin, Madison, WI 53706 (Saban, Skoyen, Fialkowski, Bjorling); the Department of Small Animal Clinical Sciences, Virginia-Maryland Regional College of Veterinary Medicine, Blacksburg, VA 24061-0442 (Broadstone); Promega Corp, Madison, WI 53711 (Haak-Frendscho); the Department of Veterinary Pathobiology, College of Veterinary Medicine, St Paul, MN 55108 (Maheswaran); and the Department of Pathobiological Sciences, School of Veterinary Medicine, University of Wisconsin, Madison, WI 53706 (Czuprynski).

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Charles Czuprynski From the Department of Surgical Sciences, School of Veterinary Medicine, University of Wisconsin, Madison, WI 53706 (Saban, Skoyen, Fialkowski, Bjorling); the Department of Small Animal Clinical Sciences, Virginia-Maryland Regional College of Veterinary Medicine, Blacksburg, VA 24061-0442 (Broadstone); Promega Corp, Madison, WI 53711 (Haak-Frendscho); the Department of Veterinary Pathobiology, College of Veterinary Medicine, St Paul, MN 55108 (Maheswaran); and the Department of Pathobiological Sciences, School of Veterinary Medicine, University of Wisconsin, Madison, WI 53706 (Czuprynski).

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DOI:
https://doi.org/10.2460/ajvr.1997.58.11.1227
Volume/Issue:
Volume 58: Issue 11
Received:
30 Dec 1996
|
Accepted:
25 Feb 1997
|
Online Publication Date:
01 Nov 1997
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SUMMARY

Objective

To identify the effect of Pasteurella haemolytica lipopolysaccharide (LPS) and leukotoxin (LKT) on spontaneous and calcium ionophore-induced histamine and inflammatory mediator release from isolated bovine lung parenchyma.

Sample Population

Lungs from 8 healthy cattle.

Procedure

Isolated bovine lung parenchyma was incubated in vitro for 2 hours with LKT or LPS, and spontaneous and induced release of inflammatory mediators was determined.

Results

LKT and LPS increased spontaneous release of histamine and leukotriene B4. In addition, incubation with LPS increased spontaneous release of prostaglandin E2. Moreover, a differential effect of the 2 toxins on calcium ionophore-induced inflammatory mediator release was observed. LKT specifically primed isolated lung parenchyma to release leukotriene B4 and thromboxane B2 in response to calcium ionophore, whereas LPS did not alter the profile of prostanoids released by bovine lung tissue exposed to calcium ionophore.

Conclusions

Pasteurella haemolytica toxins have a direct effect on bovine lung parenchyma, causing release of inflammatory mediators, which contribute to response to infection. Furthermore, bacterial toxins (LKT in this study) may sensitize tissues to the effects of other irritant stimuli, amplifying the inflammatory response. (Am J Vet Res 1997;58:1227–1231)

SUMMARY

Objective

To identify the effect of Pasteurella haemolytica lipopolysaccharide (LPS) and leukotoxin (LKT) on spontaneous and calcium ionophore-induced histamine and inflammatory mediator release from isolated bovine lung parenchyma.

Sample Population

Lungs from 8 healthy cattle.

Procedure

Isolated bovine lung parenchyma was incubated in vitro for 2 hours with LKT or LPS, and spontaneous and induced release of inflammatory mediators was determined.

Results

LKT and LPS increased spontaneous release of histamine and leukotriene B4. In addition, incubation with LPS increased spontaneous release of prostaglandin E2. Moreover, a differential effect of the 2 toxins on calcium ionophore-induced inflammatory mediator release was observed. LKT specifically primed isolated lung parenchyma to release leukotriene B4 and thromboxane B2 in response to calcium ionophore, whereas LPS did not alter the profile of prostanoids released by bovine lung tissue exposed to calcium ionophore.

Conclusions

Pasteurella haemolytica toxins have a direct effect on bovine lung parenchyma, causing release of inflammatory mediators, which contribute to response to infection. Furthermore, bacterial toxins (LKT in this study) may sensitize tissues to the effects of other irritant stimuli, amplifying the inflammatory response. (Am J Vet Res 1997;58:1227–1231)

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Cover American Journal of Veterinary Research
American Journal of Veterinary Research
Publication Date:
01 Nov 1997
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