Linkage of a microsatellite marker to the canine copper toxicosis locus in Bedlington Terriers

Vilma Yuzbasiyan-Gurkan From the Departments of Small Animal Clinical Sciences (Yuzbasiyan-Gurkan, Venta, Brewer) and Microbiology (Yuzbasiyan-Gurkan, Venta), Michigan State University, East Lansing, MI 48824, the Department of Pediatrics, University of Virgma, Charlottesville, VA 22908 (Blanton), and the Department of Human Geneties, University of Michigan, Ann Arbor, MI 48109 (Cao, Ferguson, Li, Brewer).

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Susan Halloran Blanton From the Departments of Small Animal Clinical Sciences (Yuzbasiyan-Gurkan, Venta, Brewer) and Microbiology (Yuzbasiyan-Gurkan, Venta), Michigan State University, East Lansing, MI 48824, the Department of Pediatrics, University of Virgma, Charlottesville, VA 22908 (Blanton), and the Department of Human Geneties, University of Michigan, Ann Arbor, MI 48109 (Cao, Ferguson, Li, Brewer).

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Yueying Cao From the Departments of Small Animal Clinical Sciences (Yuzbasiyan-Gurkan, Venta, Brewer) and Microbiology (Yuzbasiyan-Gurkan, Venta), Michigan State University, East Lansing, MI 48824, the Department of Pediatrics, University of Virgma, Charlottesville, VA 22908 (Blanton), and the Department of Human Geneties, University of Michigan, Ann Arbor, MI 48109 (Cao, Ferguson, Li, Brewer).

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Paul Ferguson From the Departments of Small Animal Clinical Sciences (Yuzbasiyan-Gurkan, Venta, Brewer) and Microbiology (Yuzbasiyan-Gurkan, Venta), Michigan State University, East Lansing, MI 48824, the Department of Pediatrics, University of Virgma, Charlottesville, VA 22908 (Blanton), and the Department of Human Geneties, University of Michigan, Ann Arbor, MI 48109 (Cao, Ferguson, Li, Brewer).

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Jianping Li From the Departments of Small Animal Clinical Sciences (Yuzbasiyan-Gurkan, Venta, Brewer) and Microbiology (Yuzbasiyan-Gurkan, Venta), Michigan State University, East Lansing, MI 48824, the Department of Pediatrics, University of Virgma, Charlottesville, VA 22908 (Blanton), and the Department of Human Geneties, University of Michigan, Ann Arbor, MI 48109 (Cao, Ferguson, Li, Brewer).

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Patrick J. Venta From the Departments of Small Animal Clinical Sciences (Yuzbasiyan-Gurkan, Venta, Brewer) and Microbiology (Yuzbasiyan-Gurkan, Venta), Michigan State University, East Lansing, MI 48824, the Department of Pediatrics, University of Virgma, Charlottesville, VA 22908 (Blanton), and the Department of Human Geneties, University of Michigan, Ann Arbor, MI 48109 (Cao, Ferguson, Li, Brewer).

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George J. Brewer From the Departments of Small Animal Clinical Sciences (Yuzbasiyan-Gurkan, Venta, Brewer) and Microbiology (Yuzbasiyan-Gurkan, Venta), Michigan State University, East Lansing, MI 48824, the Department of Pediatrics, University of Virgma, Charlottesville, VA 22908 (Blanton), and the Department of Human Geneties, University of Michigan, Ann Arbor, MI 48109 (Cao, Ferguson, Li, Brewer).

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Abstract

Objective

To identify a DNA marker for the copper toxicosis (CT) locus in Bedlington Terriers (BT).

Animals

77 BT, of which 25 were affected. Diagnosis of affected or unaffected with CT was made in all cases by quantitative copper determinations on liver biopsy samples by use of established criteria.

Procedure

BT pedigrees segregating for CT were identified. Linkage studies were carried out using polymorphic microsatellite markers developed for the canine genome in these pedigrees. DNA was isolated from blood samples of pedigree members. Polymerase chain reaction was used to amplify and type alleles at 213 microsatellite loci in each dog, and findings were subjected to linkage analysis.

Results

One microsatellite marker was identified to be closely linked to CT with logarithm of odds score of 5.96 at a recombination fraction of zero.

Conclusions

Using the linked marker, it has become possible to distinguish affected, homozygous normal, and carrier dogs in some BT pedigrees.

Clinical Relevance

In informative pedigrees where the marker is variable in the parents, it is possible to identify which dogs will require anticopper therapy and provide breeders with sound scientific advice about breeding strategies. (Am J Vet Res 1997;58:23–27)

Abstract

Objective

To identify a DNA marker for the copper toxicosis (CT) locus in Bedlington Terriers (BT).

Animals

77 BT, of which 25 were affected. Diagnosis of affected or unaffected with CT was made in all cases by quantitative copper determinations on liver biopsy samples by use of established criteria.

Procedure

BT pedigrees segregating for CT were identified. Linkage studies were carried out using polymorphic microsatellite markers developed for the canine genome in these pedigrees. DNA was isolated from blood samples of pedigree members. Polymerase chain reaction was used to amplify and type alleles at 213 microsatellite loci in each dog, and findings were subjected to linkage analysis.

Results

One microsatellite marker was identified to be closely linked to CT with logarithm of odds score of 5.96 at a recombination fraction of zero.

Conclusions

Using the linked marker, it has become possible to distinguish affected, homozygous normal, and carrier dogs in some BT pedigrees.

Clinical Relevance

In informative pedigrees where the marker is variable in the parents, it is possible to identify which dogs will require anticopper therapy and provide breeders with sound scientific advice about breeding strategies. (Am J Vet Res 1997;58:23–27)

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