Characterization of polypeptides synthesized and secreted by oviductal epithelial cell explants obtained from young, fertile and aged, subfertile mares

S. P. Brinsko From the Departments of Clinical Science (Brinsko, Ball, Thomas, Ellington) and Animal Sciences (Ignotz, Currie), Cornell University, Ithaca, NY 14853.

Search for other papers by S. P. Brinsko in
Current site
Google Scholar
PubMed
Close
 DVM, PhD
,
G. G. Ignotz From the Departments of Clinical Science (Brinsko, Ball, Thomas, Ellington) and Animal Sciences (Ignotz, Currie), Cornell University, Ithaca, NY 14853.

Search for other papers by G. G. Ignotz in
Current site
Google Scholar
PubMed
Close
 PhD
,
B. A. Ball From the Departments of Clinical Science (Brinsko, Ball, Thomas, Ellington) and Animal Sciences (Ignotz, Currie), Cornell University, Ithaca, NY 14853.

Search for other papers by B. A. Ball in
Current site
Google Scholar
PubMed
Close
 DVM, PhD
,
P. G. A. Thomas From the Departments of Clinical Science (Brinsko, Ball, Thomas, Ellington) and Animal Sciences (Ignotz, Currie), Cornell University, Ithaca, NY 14853.

Search for other papers by P. G. A. Thomas in
Current site
Google Scholar
PubMed
Close
 BVSc, PhD
,
W. B. Currie From the Departments of Clinical Science (Brinsko, Ball, Thomas, Ellington) and Animal Sciences (Ignotz, Currie), Cornell University, Ithaca, NY 14853.

Search for other papers by W. B. Currie in
Current site
Google Scholar
PubMed
Close
 PhD
, and
J. E. Ellington From the Departments of Clinical Science (Brinsko, Ball, Thomas, Ellington) and Animal Sciences (Ignotz, Currie), Cornell University, Ithaca, NY 14853.

Search for other papers by J. E. Ellington in
Current site
Google Scholar
PubMed
Close
 DVM, PhD

Click on author name to view affiliation information

Abstract

Objective

To compare the electrophoretic patterns of proteins synthesized and secreted by oviductal epithelial cell (OEC) explants obtained from young, fertile and aged, subfertile mares.

Animals

Young, fertile (n = 5; 2 to 7 years old) and aged, subfertile (n = 5; 17 to 24 years old) mares.

Procedure

2-dimensional sodium dodecyl sulfate-polyacrylamide gel electrophoresis and computerized densitometry.

Results

Variation in the synthesis and secretion of poly-peptides from young, fertile mare OEC (YOEC) and aged, subfertile mare OEC (AOEC) was evidenced by differences in the intensity of radiolabeled polypeptides on fluorograms. Fluorograms for 9 acidic (isoelectric point, 5.09 to 6.50) proteins of low to medium molecular mass (18.2 to 85.0 kd) from YOEC had greater intensity than did those from AOEC. Fluorograms for 7 proteins (10.5 to 45.0 kd; isoelectric point, 5.80 to 6.92) from AOEC had greater intensity.

Conclusion

The differences detected in the fluorographic intensities of secreted proteins from YOEC and AOEC may be related to the disparity in embryo development observed between young, fertile and aged, subfertile mares.

Clinical Relevance

Failure to maintain pregnancy in aged, subfertile mares may be a result of a suboptimal oviductal environment exerting its effects on the conceptus during early cleavage. (Am J Vet Res 1996;57:1346-1353)

Abstract

Objective

To compare the electrophoretic patterns of proteins synthesized and secreted by oviductal epithelial cell (OEC) explants obtained from young, fertile and aged, subfertile mares.

Animals

Young, fertile (n = 5; 2 to 7 years old) and aged, subfertile (n = 5; 17 to 24 years old) mares.

Procedure

2-dimensional sodium dodecyl sulfate-polyacrylamide gel electrophoresis and computerized densitometry.

Results

Variation in the synthesis and secretion of poly-peptides from young, fertile mare OEC (YOEC) and aged, subfertile mare OEC (AOEC) was evidenced by differences in the intensity of radiolabeled polypeptides on fluorograms. Fluorograms for 9 acidic (isoelectric point, 5.09 to 6.50) proteins of low to medium molecular mass (18.2 to 85.0 kd) from YOEC had greater intensity than did those from AOEC. Fluorograms for 7 proteins (10.5 to 45.0 kd; isoelectric point, 5.80 to 6.92) from AOEC had greater intensity.

Conclusion

The differences detected in the fluorographic intensities of secreted proteins from YOEC and AOEC may be related to the disparity in embryo development observed between young, fertile and aged, subfertile mares.

Clinical Relevance

Failure to maintain pregnancy in aged, subfertile mares may be a result of a suboptimal oviductal environment exerting its effects on the conceptus during early cleavage. (Am J Vet Res 1996;57:1346-1353)

All Time Past Year Past 30 Days
Abstract Views 0 0 0
Full Text Views 21 21 5
PDF Downloads 30 30 4
Advertisement