Reproducibility of the blood lactate-running speed curve in horses under field conditions

A. Guhl From Institut für Anatomic Physiologic und Hygiene der Haustiere, Katzenburgweg 7-9 (Guhl), and Arbeitsgruppe Pferd, c/o Im Eichholz 10 (Lindner, von Wittke), Bonn, Germany.

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A. Lindner From Institut für Anatomic Physiologic und Hygiene der Haustiere, Katzenburgweg 7-9 (Guhl), and Arbeitsgruppe Pferd, c/o Im Eichholz 10 (Lindner, von Wittke), Bonn, Germany.

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P. von Wittke From Institut für Anatomic Physiologic und Hygiene der Haustiere, Katzenburgweg 7-9 (Guhl), and Arbeitsgruppe Pferd, c/o Im Eichholz 10 (Lindner, von Wittke), Bonn, Germany.

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Abstract

Objective

To examine the reproducibility of blood lactate-running speed curve parameters derived by a curve-fit equation and by linear interpolation from the results of 4-speed tests of sport horses under field conditions.

Design

Thoroughbreds completed 10 test pairs with 3 to 11 days between test and retest.

Animals

7 Thoroughbreds.

Procedure

The 4-speed test consisted of 4 runs over a distance of 2,110 m. Exercise intensity was increased by 1 m/s for each run. Blood lactate concentration measured after each run was plotted against running speed to determine the blood lactate-running speed relation. By means of the blood lactate-running speed relation, velocity inducing 2, 2.5, 3, 3.5, or 4 mM blood lactate concentration was calculated by a curve-fit equation and by linear interpolation.

Results

The test-retest correlation coefficient of velocity inducing 2, 2.5, 3, 3.5, and 4 mM blood lactate concentration determined by curve-fit equation was 0.67, 0.78, 0.84, 0.86, and 0.86, respectively, and by linear interpolation was 0.42, 0.58, 0.77, 0.87, and 0.78, respectively. But the test-retest regression was equal to the line of identity only for velocity inducing 3.5 and 4 mM blood lactate concentration.

Conclusions

The reproducibility of velocity inducing 3.5 and 4 mM blood lactate concentration was sufficient to be used to compare horses and evaluate conditioning effects. This holds for both methods of calculation of the parameters derived from the blood lactate-running speed relation. (Am J Vet Res 1996;57:1059–1062)

Abstract

Objective

To examine the reproducibility of blood lactate-running speed curve parameters derived by a curve-fit equation and by linear interpolation from the results of 4-speed tests of sport horses under field conditions.

Design

Thoroughbreds completed 10 test pairs with 3 to 11 days between test and retest.

Animals

7 Thoroughbreds.

Procedure

The 4-speed test consisted of 4 runs over a distance of 2,110 m. Exercise intensity was increased by 1 m/s for each run. Blood lactate concentration measured after each run was plotted against running speed to determine the blood lactate-running speed relation. By means of the blood lactate-running speed relation, velocity inducing 2, 2.5, 3, 3.5, or 4 mM blood lactate concentration was calculated by a curve-fit equation and by linear interpolation.

Results

The test-retest correlation coefficient of velocity inducing 2, 2.5, 3, 3.5, and 4 mM blood lactate concentration determined by curve-fit equation was 0.67, 0.78, 0.84, 0.86, and 0.86, respectively, and by linear interpolation was 0.42, 0.58, 0.77, 0.87, and 0.78, respectively. But the test-retest regression was equal to the line of identity only for velocity inducing 3.5 and 4 mM blood lactate concentration.

Conclusions

The reproducibility of velocity inducing 3.5 and 4 mM blood lactate concentration was sufficient to be used to compare horses and evaluate conditioning effects. This holds for both methods of calculation of the parameters derived from the blood lactate-running speed relation. (Am J Vet Res 1996;57:1059–1062)

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